Viral RNA Isolation Kit ya Viral RNA Purification kuchokera ku Plasma, Seramu, ndi Zitsanzo Zina
Kufotokozera Kwa Zida:
Cat.No.RE-02011/02014
Kuyeretsedwa kwa ma virus a RNA kuchokera ku plasma, seramu, madzi amthupi opanda ma cell, zamphamvu zama cell.
Patulani mwachangu ndikuyeretsa ma virus a RNA kuchokera ku zitsanzo monga plasma, seramu, madzi am'thupi opanda ma cell ndi zida zapamwamba zama cell.
-Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.Zida zonse ndi RNase-Free
-Zosavuta-zochita zonse zimatsirizidwa ndi kutentha kwa chipinda
-Kuthamanga-ntchito imatha kutha pakatha mphindi 20
-Zokolola zambiri za RNA: Mzere wa RNA wokha komanso mawonekedwe apadera amatha kuyeretsa RNA bwino
- Otetezeka-palibe organic reagent yomwe imagwiritsidwa ntchito
-Large chitsanzo processing mphamvu-mpaka 200μl zitsanzo akhoza kukonzedwa nthawi iliyonse.
-Zapamwamba kwambiri - RNA yoyeretsedwa ndi yoyera kwambiri, yopanda mapuloteni ndi zonyansa zina, ndipo imatha kukumana ndi ntchito zosiyanasiyana zoyesera.
Kufotokozera
Chidacho chimagwiritsa ntchito spin column ndi formula yopangidwa ndi Foregene, yomwe imatha kutulutsa ma virus a RNA apamwamba kwambiri komanso apamwamba kwambiri kuchokera ku zitsanzo monga plasma, seramu, madzi amthupi opanda ma cell, komanso ma cell cell supernatant.Chidacho chimawonjezera Linear Acrylamide, yomwe imatha kujambula mosavuta RNA pang'ono kuchokera pazitsanzo.RNA-Only Column imatha kumangirira RNA moyenera.Chidachi chimatha kukonza zitsanzo zambiri nthawi imodzi.
Zida zonse zilibe RNase, kotero RNA yoyeretsedwa sidzanyozedwa.Buffer viRW1 ndi Buffer viRW2 zitha kuwonetsetsa kuti ma viral nucleic acid omwe apezeka opanda mapuloteni, ma nuclease kapena zonyansa zina, zomwe zitha kugwiritsidwa ntchito mwachindunji pakuyesa kwazachilengedwe kwa maselo.
Zofotokozera
50 Preps, 200 Preps
Zida zamagulu
| Linear Acrylamide |
| Buffer viRL |
| Buffer viRW1, Buffer viRW2 |
| RNase-Free ddH2O |
| RNA-Only Column |
| Malangizo |
Mbali & ubwino
-Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.Zida zonse ndi RNase-Free
-Zosavuta-zochita zonse zimatsirizidwa ndi kutentha kwa chipinda
-Kuthamanga-ntchito imatha kutha pakatha mphindi 20
-Zokolola zambiri za RNA: Mzere wa RNA wokha komanso mawonekedwe apadera amatha kuyeretsa RNA bwino
- Otetezeka-palibe organic reagent yomwe imagwiritsidwa ntchito
-Large chitsanzo processing mphamvu-mpaka 200μl zitsanzo akhoza kukonzedwa nthawi iliyonse.
-Zapamwamba kwambiri - RNA yoyeretsedwa ndi yoyera kwambiri, yopanda mapuloteni ndi zonyansa zina, ndipo imatha kukumana ndi ntchito zosiyanasiyana zoyesera.
Zosintha za Kit
Pulogalamu ya Kit:
Ndi oyenera m'zigawo ndi kuyeretsedwa kwa tizilombo RNA mu zitsanzo monga plasma, seramu, maselo opanda madzimadzi a m'thupi ndi selo chikhalidwe supernatant.
Kuyenda kwa ntchito
Zosungirako
- Zida zitha kusungidwa kwa miyezi 24 kutentha (15-25 ℃), kapena 2-8 ℃ kwa nthawi yayitali.
-Linear Acrylamide yankho akhoza kusungidwa firiji kwa masiku 7.Mukalandira zida, chonde tengani njira ya Linear Acrylamide ndikuyisunga pa -20 ℃.
-Mukawonjezera Linear Acrylamide ku Buffer viRL, imatha kusungidwa ku 2-8 ℃ mpaka 48h.Chonde gwiritsani ntchito njira yokonzekera mwatsopano.
Malangizo owunikira mavuto
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Palibe RNA yomwe ingatulutsidwe kapena zokolola za nucleic acid ndizochepa
Nthawi zambiri pamakhala zinthu zambiri zomwe zimakhudza kuchira bwino, monga: zitsanzo za RNA, njira yogwirira ntchito, voliyumu, etc..
Kusanthula kwazomwe zimayambitsa:
1.Ice kusamba kapena otsika kutentha (4 ° C) centrifugation pa ntchito.
Malangizo: Kutentha kwachipinda (15-25 ° C), osasamba madzi oundana komanso kutentha kwa centrifuge.
2. Kusungidwa kwachitsanzo kosayenera kapena kusungirako zitsanzo kwa nthawi yayitali.
Malangizo: Sungani zitsanzo pa -80 ° C kapena kuzizira mu nayitrogeni wamadzimadzi, ndipo pewani kugwiritsa ntchito kuzizira kobwerezabwereza;yesani kugwiritsa ntchito zitsanzo zomwe zasonkhanitsidwa mwatsopano kuti muchotse RNA.
3.Kusakwanira kwachitsanzo lysis
Malangizo: Chonde onetsetsani kuti zitsanzo ndi njira yogwirira ntchito (Linear Acrylamide) yasakanizidwa bwino ndikuyimitsidwa kwa mphindi 10 kutentha (15-25 ° C)
4.Chidziwitso chinawonjezedwa molakwika
Malangizo: Onetsetsani kuti RNase-Free ddH2O yawonjezedwa pakati pa nembanemba ya gawo loyeretsa.
5.Voliyumu yolakwika ya ethanol ya anhydrous mu Buffer viRW2
Yesani: Chonde tsatirani malangizo, onjezani voliyumu yolondola ya ethanol ya anhydrous ku Buffer viRW2 ndikusakaniza bwino musanagwiritse ntchito zida.
6.Kugwiritsira ntchito chitsanzo molakwika.
Malingaliro: 200µl ya zitsanzo pa 500μl ya Buffer viRL.Kuchuluka kwachitsanzo kumabweretsa kuchepa kwa RNA m'zigawo.
7. Voliyumu yolakwika kapena kusakwanira.
Lingaliro: Voliyumu yosawoneka bwino ya gawo loyeretsera ndi 30-50μl;ngati mphamvu ya elution siyikukhutiritsa, tikulimbikitsidwa kuwonjezera RNase-Free ddH yotenthetserapo.2O ndikuwonjezera nthawi yoyika kutentha, monga 5-10min
8.Kuyeretsa kumakhala ndi zotsalira za ethanol mutatsuka mu Buffer viRW2.
Lingaliro: Ngati Mowa utsalirabe mutatha kutsuka mu Buffer viRW2 ndi centrifugation yopanda kanthu-chubu kwa 2min, ndime yoyeretsera imatha kusiyidwa kutentha kwa mphindi 5 pambuyo pa chubu chopanda kanthu kuti muchotse ethanol yotsalira.
Kuwonongeka kwa mamolekyu oyeretsedwa a RNA
Ubwino wa RNA yoyeretsedwa umagwirizana ndi zinthu monga kusungirako zitsanzo, kuipitsidwa kwa RNase, ndi ntchito.
Kusanthula kwazomwe zimayambitsa:
1.Zitsanzo zosonkhanitsidwa sizinasungidwe mu nthawi.
Yesani: Ngati chitsanzocho sichigwiritsidwa ntchito pakapita nthawi, chonde sungani pa -80 ℃ kapena nayitrogeni wamadzimadzi nthawi yomweyo.Pochotsa mamolekyu a RNA, yesani kugwiritsa ntchito zitsanzo zomwe zasonkhanitsidwa mwatsopano ngati kuli kotheka.
2.Zitsanzo zosonkhanitsa zinali kuzizira ndi kusungunuka mobwerezabwereza.
Yesani: Pewani kuzizira kobwerezabwereza ndi kusungunuka (osapitirira kamodzi) panthawi yosonkhanitsa ndi kusunga, apo ayi zokolola za nucleic acid zidzachepa.
3.RNase idayambitsidwa m'chipinda chopangira opaleshoni kapena palibe magolovesi otaya, masks, ndi zina zambiri.
Lingaliro: Kutulutsa kwa kuyesa kwa mamolekyu a RNA kumachitidwa bwino m'chipinda chapadera cha RNA, ndipo tebulo loyesera limatsukidwa kuyesako kusanachitike.Valani magolovesi ndi masks otayika panthawi yoyesera kuti mupewe kuwonongeka kwa RNA komwe kumachitika chifukwa cha kuyambika kwa RNase.
4.The reagent imakhudzidwa ndi RNase panthawi yogwiritsira ntchito.
Yesani: Bwezerani ndi Viral RNA Isolation Kit yatsopano pazoyeserera zofananira.
5.Kuwonongeka kwa RNase kwa machubu a centrifuge, nsonga za pipette, ndi zina zotero.
Mamolekyu oyeretsedwa a RNA adakhudza zoyeserera zakutsika
Mamolekyu a RNA oyeretsedwa ndi gawo loyeretsera adzakhudza kuyesa kumtunda ngati pali ma ion amchere ambiri kapena mapuloteni, monga: reverse transcript, Northern Blot, etc..
1.Pali ma ion amchere otsala mu ma molekyulu a RNA opangidwa.
Malangizo: Onetsetsani kuti voliyumu yolondola ya ethanol ya anhydrous yawonjezedwa ku Buffer viRW2, ndikutsuka ndime yoyeretsa kawiri molingana ndi liwiro lolondola la centrifugation pamalangizo opangira; Ngati pali ma ion amchere otsala, mutha kuwonjezera Buffer viRW2 pagawo loyeretsera, ndikuyisiya kutentha kwa 5min.Ndiye kuchita centrifugation kuchotsa mchere ayoni kuipitsidwa kwambiri
2.Pali ethanol yotsala mu mamolekyu a RNA opangidwa
Lingaliro: mutatsimikizira kuti mizati yoyeretsera yatsukidwa ndi Buffer viRW2, chitani chopanda kanthu-chubu centrifugation malinga ndi liwiro la centrifugal pa malangizo ogwiritsira ntchito.Ngati pali Mowa otsala, akhoza kusiyidwa kwa mphindi 5 kutentha firiji pambuyo chopanda kanthu chubu centrifugation kuchotsa Mowa otsala kumlingo waukulu.
Mabuku a Malangizo:
Viral RNA Isolation Kit Instruction Manual
