China Mbidzi Nsomba Direct PCR Kit Direct PCR Lysis Reagent(mbidzi) Wopanga ndi Wopereka |Foregene

Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent (mbidzi)

Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent(zebrafish) Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Gwiritsani ntchito mwachindunji zebrafish lysate ngati template yakukulitsa kwa PCR, popanda kutulutsa kosiyana kwa DNA, kufulumira komanso kukhudzika kwambiri.

◮Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

◮Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

◮Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Titumizireni imelo Tsitsani ngati PDF

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Izi zimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwamsanga DNA ya genomic kuchokera ku zebrafish ndi nsomba zina zamadzi amchere, zipsepse za mchira kapena mazira a nsomba zomwe zimachitika pa PCR, choncho ndizoyenera kwambiri kuyesa chibadwa chachikulu.Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65 ° C.Palibe njira zina monga kuchotsedwa kwa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2 × PCR Yosavuta^TMMix ili ndi kulolerana kwamphamvu kwa PCR reaction inhibitors, ndipo imatha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yokulitsa bwino komanso yeniyeni.Reagent iyi ili ndi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl₂, zotchingira zochita, PCR optimizer ndi stabilizer.Kugwiritsidwa ntchito molumikizana ndi chotchinga cha lysis kumatha kuzindikira mwachangu komanso mosavuta zitsanzo, ndipo kumakhala ndi mawonekedwe amphamvu kwambiri, kukhazikika kwamphamvu, komanso kukhazikika bwino.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	FP yoyipa
	Foregene Protease
	6 × DNA Loading Buffer
Gawo II	2 × PCR Yosavuta^TMSakanizani
Malangizo

Mbali & ubwino

■Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

■ Zofuna zachitsanzo ndizochepa, monga 1mg nsomba ya caudal fin kapena mazira 10 a nsomba angayesedwe.

■ Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo opaleshoniyo ndi yosavuta.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Zosintha za Kit

Kugwiritsa ntchito: Nsomba za Zebra ndi nsomba zina zam'madzi.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer FP ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

v Reagent Foregene Protease ali ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

vReagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

vReagent 2 × PCR EasyTM Mix, ngati imagwiritsidwa ntchito kawirikawiri, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (ntchito mkati mwa masiku 10).

Zam'mbuyo: ForeSNP Genotyping Kit

Ena: Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(mbidzi)

Lembani uthenga wanu apa ndikutumiza kwa ife