DirectPCR ndi chiyani?
Ukadaulo wa DirectPCR umatanthawuza kuti popanda kulekanitsa ndi kuyeretsa ma nucleic acid mamolekyulu (kuphatikiza DNA ndi RNA) yamitundu yosiyanasiyana ya minofu, minofu ndi mawonekedwe a cell okha amawonongedwa ndi lysis ya protease, nucleic acid imatulutsidwa mu njira ya lysis, ndipo yankho la lysis limawonjezedwa mwachindunji.PCR reaction system ndiukadaulo wokulitsa jini yomwe mukufuna.
Kuyang'ana zosintha
Kupatukana ndi kutulutsa kwa nucleic acids nkhani
Chiyambireni kupangidwa kwaukadaulo wa PCR zaka 30 zapitazo, ofufuza akhala akuvutika ndi kulekanitsidwa ndi kutulutsa ma nucleic acid.Kugwiritsa ntchito zitsanzo za minofu kuti achite mwachindunji machitidwe a PCR ndiloto la ofufuza ambiri.Koma maloto amenewa sanakwaniritsidwe kwa zaka 30.Chifukwa chake ndikuti minofu yowonongeka idzatulutsa zinthu zambiri zolepheretsa.
Izi zoletsa zigawo zikuluzikulu adzakhala ndi amphamvu chopinga zotsatira pa anachita PCR, ena adzachititsa zoyamba ndi Chinsinsi kuti sangathe kumanga, ena ndi amphamvu mapuloteni denaturation ntchito, chifukwa inactivation wa nucleic asidi polymerase, ndi ena mwachindunji kuteteza zoyamba ndi template kuti kumanga .Izi ndizinthu zonse zomwe zimapangitsa kuti PCR isayende bwino.
Mwachizoloŵezi, anthu nthawi zina amagwiritsa ntchito zowonjezera za PCR kuti apititse patsogolo chidwi, kukhazikika komanso kukulitsa mphamvu za PCR.Komabe, chifukwa cha mitundu yosiyanasiyana ya PCR inhibitors yomwe ili mu ma templates a nucleic acid kuchokera ku magwero osiyanasiyana, kugwiritsa ntchito nthawi yaitali kwa zowonjezera zowonjezera mwachiwonekere sikuli koyenera kutayika, mtengo wake ndi wokwera, ndipo ntchitoyo ndi yovuta.
Foregene DirectPCR
Kupambana kwakukulu padziko lonse lapansi --Two Techniques
Foregene apanga zotsogola m'munda waukadaulo uwu padziko lonse lapansi.Ukadaulo wa Foregene DirectPCR uli ndi mfundo ziwiri zaukadaulo, zomwe zimapangitsa Foregene kukhala mpainiya komanso mtsogoleri waukadaulo wa DirectPCR.
Choyamba, patented nucleic acid polymerase kusinthidwa njira.Forgene ali ndi patented nuclease modification njira, yomwe imayang'ana pamalo omwe amamangiriza kuti nyukiliya ndi template ikhale yamphamvu, yokhala ndi zochitika zapamwamba, kuchita bwino kwambiri komanso kukhazikika kwamafuta.
Chachiwiri, formula ya PCR Mix yokometsedwa yamitundu yosiyanasiyana, zowonjezera zomwe zimapangidwira, zowonjezera ndi zokhazikika, zimakulitsa kukana kwa ma polymerase ku PCR inhibitors ndikuwonetsetsa kuti kukulitsa bwino.
Ndi chifukwa cha zochitika ziwiri zomwe zatchulidwa pamwambapa zomwe Foregene adazindikira DirectPCR yeniyeni padziko lonse lapansi.Kaya ndi nyama wamba, madzi a m'thupi, minyewa ya mbewu, masamba kapena nsonga za mizu, kapena mbewu za mbewu, ogwiritsa ntchito amatha kukwaniritsa kukulitsa kwa PCR mwachindunji popanda kusweka kwa makina kapena kuyeretsa kwa nucleic acid.
Kwa zida za DirectPCR za mndandanda wa zomera ndi zinyama zomwe zakhazikitsidwa, Foregene akhoza kunena monyadira kuti tili ndi zizindikiro zotsogola padziko lonse lapansi.M'tsogolomu, Foregene adzapitiriza kukhazikitsa mndandanda wa zinthu zomwe zidakali ndi zizindikiro zotsogola padziko lonse lapansi (kapena ngakhale zapadera).
Kuti mudziwe zambiri, lowetsani:
http://www.foregene.com/http://www.foreivd.com/
Nthawi yotumiza: Feb-27-2017
