◮Kukhulupirika kwakukulu: nthawi 6 kuposa enzyme wamba ya Taq;
◮Liwiro lokulitsa mwachangu
◮Zambiri zosinthika za template
◮Kuchita bwino kwambiri kwa amplification
◮Kulekerera kwachilengedwe kumakhala kolimba: kuyikidwa pa 37 ° C kwa sabata, kusunga ntchito zoposa 90%;
◮Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.