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Lnc-RT Heroᵀᴹ I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)
Super Premix for first-strand cDNA synthesis from lncRNA
Cat.No.RTL-09098/09099
Makina osindikizira ofulumira komanso omvera kwambiri kuti apange cDNA yoyamba kuchokera ku lncRNA.
Chotsani gDNA mu mphindi ziwiri.
Mphindi 15 kuti amalize kaphatikizidwe ka chingwe choyamba cDNA
High-sensitivity reverse transcription system
Kukhazikika kwamafuta ambiri, kutentha kwabwino kwambiri ndi 42 ℃, ndipo kumakhalabe ndi kalembedwe kabwino ka 50 ℃.
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RT Easyᵀᴹ I Master Premix yopanga chingwe choyamba cha cDNA
- Makina olembera osinthika, zimangotenga mphindi 25 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
- High-sensitivity reverse transcription system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
- Makina osindikizira osinthika amakhala ndi kukhazikika kwamafuta ambiri, kutentha kwamachitidwe kumatha kufika 50 ℃,ndipo ili ndi magwiridwe antchito abwino osinthira.
- Ngakhale zitsanzo zonyansa za RNA (mowa mpaka 45%, mchere wa guanidine mpaka 750mM) ukhoza kusinthidwanso.
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RT Easy II (yokhala ndi gDNase) Master Premix ya chingwe choyamba cha cDNA kaphatikizidwe ka Real Time PCR yokhala ndi gDNase
-Kutha kuchotsa gDNA, yomwe imatha kuchotsa gDNA mu template mkati mwa mphindi ziwiri.
-Njira yabwino yolembera, zimangotenga mphindi 15 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
-Ma tempulo ovuta: ma tempulo okhala ndi GC yayikulu komanso mawonekedwe achiwiri ovuta amathanso kusinthidwa ndikuchita bwino kwambiri.
-High-sensitivity reverse transcript system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
-Makina olembera kumbuyo amakhala ndi kukhazikika kwamafuta, kutentha koyenera ndi 42℃, ndipo ikadali ndi ntchito yabwino yosinthira ku 50℃.
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RT Easyᵀᴹ II Master Premix ya chingwe choyamba cha cDNA kaphatikizidwe ka Real Time PCR
- Makina olembera osinthika, zimangotenga mphindi 20 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
- High-sensitivity reverse transcription system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
- Makina osindikizira osinthika amakhala ndi kukhazikika kwamafuta ambiri, kutentha kwadongosolo kumatha kufika mpaka 50 ℃, ndipo imakhala ndi ntchito yabwino yolembera.
- Ngakhale zitsanzo zonyansa za RNA (mowa mpaka 60%, mchere wa guanidine mpaka 750mM) ukhoza kusinthidwanso.
- 2 × RT OR-YosavutaTM Mix yawonjezera zoyambira zosinthira (Random Primer, Oligo(dT)18 Choyamba).
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