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01

Ngati: 17.694

Lofalitsidwa ndi: The First Affiliated Hospital, Sun Yat-sen University

Zomwe zili zofunika kwambiri: Esophageal squamous cell carcinoma (ESCC) ndiye mtundu waukulu wa khansa yam'mero.Kuzindikira zolinga zochizira ndizofunikira kwambiri pakuchiza bwino kwa ESCC.Kafukufuku wina wasonyeza kuti kusokonezeka kwa RNA kumatha kulimbikitsa kufotokozera ndi kumasulira kwa ma mRNA okhudzana ndi khansa, koma makina a maselo sakudziwika bwino.Kafukufukuyu adawonetsa kuti mapuloteni okhudzana ndi tRNA m7G methyltransferase okhudzana ndi METTL1 ndi WDR4 adawonjezeka kwambiri m'magulu a ESCC ndipo adathandizira kuti ESCC iwonongeke.Kuyambira nthawi imeneyo, maphunziro a gene knockout a METTL1 ndi WDR4 mu mbewa zamtundu wa ESCC atsimikizira kuti ntchito yowonjezera ya tRNA m7G methyltransferase imatsogolera ku ndondomeko ya mawu a mapuloteni awiriwa, omwe amalimbikitsa kuchulukitsa kwa maselo a ESCC.Kafukufukuyu akuwonetsa ntchito yofunikira ya oncogenic yosintha molakwika tRNA m7G mu ESCC ndipo ikuwonetsa kuti kulunjika kwa METTL1 ndi njira zake zowonetsera kumunsi kungakhale kulonjeza zochizira za ESCC therapy.

Zothandizira:

Mouse Tail Direct PCR Kit Foregene#TP-01333

Ulalo woyambirira:

https://www.nature.com/articles/s41467-022-29125-7

02

Ngati: 14.903

Lofalitsidwa ndi: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau

Zambiri:

Ndi chitukuko chaukadaulo wopangira, mRNA yakhala gulu latsopano lamankhwala ochizira.Komabe, kupeza bwino kwa intracellular mankhwala a mRNA kumakhalabe kovuta chifukwa cha kukhudzika kwa mRNA ku RNases.Kafukufukuyu akupereka njira yabwino yoperekera ma polyphenol-conjugated nanostructures omwe amatha kutulutsa bwino IL-10 mRNA mu vivo.Pambuyo pogwiritsira ntchito nanostructure iyi kuti apereke mankhwala a mRNA, ma IL-10 anali oyendetsedwa bwino, akuletsa bwino kufotokoza kwa zinthu zotupa, kulimbikitsa kukonzanso kwa mucosal, kuteteza maselo a colonic epithelial kuchokera ku apoptosis, ndi kuletsa dextran sodium sulfate-induced acute and chronical disease for the urine colitis geneis .

Zothandizira:

Animal Total RNA Isolation Kit Foregene#RE-03011

Ulalo woyambirira:

Chigawo 20colitis%20model.%202.

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Ngati: 12.499

Lofalitsidwa ndi: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University

Zambiri:

Kupsyinjika kwa okosijeni kwa umuna ndi chinthu chachikulu chomwe chimatsogolera ku kusabereka kwa amuna, kotero ndizotheka komanso njira yabwino yokhazikitsira zitsanzo za nyama zosabereka za amuna posintha dongosolo la antioxidant m'njira yolunjika.Komabe, njira za antioxidant zamtundu wa majeremusi za amuna sizinafotokozedwe.Kafukufukuyu adatsimikizira kuti jini ya LanCL1 ndi jini yapadera ya antioxidant m'maselo a majeremusi aamuna, ndipo mawonekedwe ake amachepetsedwa kapena amagwirizana ndi kusabereka kwa amuna.Makoswe aamuna omwe alibe jini ya LanCL1 amawonetsa kuwonongeka kwa umuna wa okosijeni komanso kulephera kubereka.M'kati mwa masiyanidwe a umuna wa maselo a majeremusi, chizolowezi cha okosijeni chikachitika, mapuloteni 1 (SP1) amtundu wa 1 (SP1) amayang'anira mafotokozedwe a jini ya LanCL1, potero amalepheretsa njira yotulutsa okosijeni ya umuna.Kafukufukuyu akuwonetsa kuti njira ya SP1-LanCL1 imagwira ntchito yofunika kwambiri pakuwongolera ma testicular homeostasis ndi kubereka kwa amuna molumikizana ndi redox balance, ndipo akuwonetsa kuti mbewa zosinthidwa ndi LanCL1 zimakhala ndi mawonekedwe owoneka bwino ngati chinyama cha kusabereka kwa amuna.

Zothandizira:

Mouse Tail DNA Mini Kit Foregene#DE-05211

Ulalo Woyambirira:

http://europepmc.org/article/MED/35469022

Mouse Tail Direct PCR Kit

◮Palibe nthawi komanso mtengo woyeretsa DNA womwe umafunika.

◮Zitsanzo zofunidwa ndizochepa, ingotengani mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo opaleshoniyo ndi yosavuta.

◮Dongosolo la PCR lokhathamiritsa limathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolera mwamphamvu kwa PCR reaction inhibitors.

Animal Total RNA Isolation Kit

◮Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.Dongosolo lonse ndi RNase-Free.

◮Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column.

◮Chotsani DNA popanda kuwonjezera DNase.

◮Zosavuta—zochita zonse zimamalizidwa ndi kutentha kwa chipinda.

◮Opaleshoni yofulumira—itha kutha m’mphindi 30.

◮Safe—palibe organic reagent yomwe imagwiritsidwa ntchito.

◮Kuyera kwambiri—OD260/280≈1.8-2.1.

Mouse Tail DNA Mini Kit

◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.

◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.

◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.

◮Chitetezo:Palibe organic reagent m'zigawo zofunika.

◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.

◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.