Mukudziwa bwanji

Za Nkuchotsa ucleic acid

Chiyambi ndi mapeto a oyeretsedwa nucleic acid

Chilichonse chimakhala chovuta poyambira, kuyeretsedwa kwa nucleic acid ndikwambiri

Kuyambitsa kuyesa kwa ma molekyulu, pazoyeserera zotsatila

Kuchita bwino kapena kulephera kumakhudza kwambiri.

Trace/ultra-trace UV spectrophotometer imakondedwa ndi akatswiri ambiri asayansi chifukwa imatha kuzindikira kuchuluka kwa ma nucleic acid ndi mapuloteni ndi zotsalira za ayoni zamchere mosavuta, mwachangu komanso mwachuma.

Monga tonse tikudziwa, A260 amatanthauza nucleic acid absorbance OD value, A280 amatanthauza kuti protein concentration OD value, A230 imatanthawuza kuti salt ion concentration ya OD value, kotero A260 imatha kusintha nucleic acid concentration, A260/280 imatanthawuza zotsalira za protein, A260/230 imatanthawuza kuti mchere wa ion umakhala wotsalira, koma zotsatira zake ndizomwe zimangopezeka muzofufuza.wamba” kukhulupirira kuti lingathe kufotokoza chiyero cha DNA ndi RNApamlingo wakutiwakuti.Sichiyembekezo chokha chodziwikiratu zokolola za nucleic acid ndi chiyero, chimangogwiritsidwa ntchito ngati chofotokozera, ndipo si "golide".

Buku la Thermo Fisher ND-2000 limatsindika kudalirika kwa zotsatira za mayeso

Chifukwa chake ndi chakuti zotsatira zomwe zapezedwa pogwiritsa ntchito micro/ultramicro UV spectrophotometer zimangowonetsa zokolola ndi chiyero cha nucleic acid kuchokera kumbali, ndipo pali zinthu zambiri zomwe zimakhudza (njira ya kuwala, voliyumu ya chitsanzo, kuyika kwa zitsanzo, pH mtengo, ma ion ena omwe amakhudza muyeso wa absorbance, etc.) , mtengo wa OD woyezedwa siwolondola.Panthawi imodzimodziyo, chifukwa cha kusowa kwapadera kwa zinthu zodziwikiratu za kuyamwa kwamtengo wapatali, DNA yamtundu umodzi, DNA yamitundu iwiri, RNA, dNTPs, ndi mapuloteni ena onse ali ndi nsonga za kuyamwa pa 260nm wavelength, ndi ndende yomwe imatsimikiziridwa ndi A260 yokha sikutanthauza DNA yeniyeni kapena RNA.Kukhazikika, ndi kukhulupirika kwake ndi kunyozeka kwake sikungaweruzidwe.

Ndiye momwe tingaweruzire mtundu wa asidi athu oyeretsedwa a nucleic?Kuwonjezera pa kugwiritsa ntchito micro/ultramicro UV spectrophotometer kuti azindikire, njira monga agarose gel electrophoresis zimafunikanso kuti ziwonetsere chiyero ndi kukhulupirika kwa nucleic acid, ndikuwonetsa ndende pamlingo wina.Mwanjira imeneyi, zokolola za nucleic acid ndi chiyero zomwe zimapezedwa kuchokera pazotsatira za njira zosiyanasiyana ndizodalirika.

Kuyerekeza tchati choyesera

Genomic DNA ya maselo a H1299 adatengedwa pogwiritsa ntchito zida za kampani ya A's DNA Extraction, ndipo kuipitsidwa kwakukulu kunawoneka, zomwe zinachititsa kuti OD ikhale yotsika kwambiri.

(Muyeso wa OD ndi electropherogram yofananira)

Evaluation index

Kodi pali "muyezo wagolide" wa zokolola za nucleic acid ndi kuyesa kwabwino?

Monga tikudziwira, palibe njira yosavuta, yachangu komanso yotsika mtengo.Kaya ndi spectrophotometer, gel electrophoresis, kapena mass spectrometry, zonsezi zimasonyeza ndende ndi chiyero cha nucleic acid mu yankho kuchokera kuzinthu zosiyanasiyana, ndipo ayenera kuweruzidwa potengerana wina ndi mzake.

Mlozera wabwino kwambiri nthawi zonsentchito yoyeserera yotsatila.Izi sizimakhudza magwiridwe antchito a reverse transcription and PCR amplification.Kupeza zinthu zodalirika zokulirakulira ndi ma Ct, ndikupeza kutsata kwathunthu ndikutsata ndikuchotsa bwino kwa nucleic acid.

Mwachidule, lingaliro la chiphunzitso cha chiŵerengero-chokha liyenera kutsutsidwa ndi lingaliro la "kugwiritsa ntchito zotsatira zabwino zoyesera zotsika ngati magawo abwino a m'zigawo"!

Zida zopangira za Foregene DNA RNA kuti mukhale oyera kwambiri a DNA/RNA:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/