Mpaka pa June 25, 2021, National Health Commission of China idatulutsa zidziwitso zomwe zikuwonetsa kuti anthu opitilira 630 miliyoni alandila katemera mdziko langa, zomwe zikutanthauza kuti katemera wa anthu onse ku China apitilira 40%, yomwe ndi gawo lofunikira pakukhazikitsa chitetezo chamagulu .

Anthu ambiri adzakhala ndi nkhawa kuti angadziwe bwanji ngati apanga ma antibodies atalandira katemera watsopano wa korona?

Pakadali pano, zida zodziwika bwino kwambiri zodziwira ma antibody pamsika ndi zida zodziwira za antibody za IgM/IgG (njira ya golide ya colloidal).

Coronavirus (COV) ndi banja lalikulu la ma virus omwe amayambitsa matenda kuyambira chimfine mpaka matenda oopsa kwambiri monga acute kupuma kwapang'onopang'ono (SARS-CoV).SARS-CoV-2 ndi mtundu watsopano womwe sunapezekepo mwa anthu kale."Coronavirus matenda 2019" (COVID-19) amayamba ndi kachilombo ka "SARS-COV-2" matenda."Odwala a SARS-CoV-2 adanenanso zofatsa (kuphatikiza odwala ena omwe sananene zazizindikiro) mpaka zowopsa.Zizindikiro za COVID -19 zimawonekera monga kutentha thupi, kutopa, chifuwa chowuma, kupuma movutikira ndi zizindikiro zina, zomwe zimatha kukhala chibayo chachikulu, kulephera kupuma, kugwedezeka kwamadzi, kulephera kwa ziwalo zingapo, vuto la metabolism ya acid-base, ndi zina zambiri.

Zida zatsopano zodziwira ma anti-coronavirus IgM/IgG zidapangidwa kuti zizizindikira bwino ma antibodies a SARS-CoV-2 ndikuzigwiritsa ntchito ngati chida chothandizira kuzindikira matenda a SARS-CoV-2.

Mfundo yodziwira

Chidacho chili ndi (1) kuphatikiza zolembera za neocoronavirus antigen ndi zolembera zama protein zowongolera komanso (2) mizere iwiri yodziwikiratu (T1 ndi T2, motsatana yophimbidwa ndi anti-anthu IgM ndi IgG antibodies) ndi mzere wowongolera khalidwe (kuphatikiza Ndi anti-quality control protein antibody).Chitsanzocho chikawonjezedwa pamzere woyeserera, puloteni yolembedwanso ndi golide ya SARS-CoV-2 imamanga ku ma virus a IgM ndi/kapena a IgG omwe amapezeka pachitsanzocho kuti apange antigen-antibody complex.Maofesiwa amayenda pamzere woyeserera, kenako amatengedwa ndi anti-anthu antibody IgM pamzere wa T1, ndi/kapena ndi anti-anthu IgG antibody pamzere wa T2, gulu lofiirira-lofiira limawonekera pamalo oyesera, kuwonetsa zotsatira zabwino.Ngati palibe anti-SRAS-CoV-2 anti-anti-SRAS-CoV-2 mu chitsanzo kapena mulingo wa anti-anti-muchitsanzo uli wochepa kwambiri, sipadzakhala mizere yofiirira yofiira pa “T1 ndi T2″."Mzere wowongolera khalidwe" umagwiritsidwa ntchito poyang'anira ndondomeko.Ngati kuyesaku kukuyenda bwino ndipo ma reagents akugwira ntchito bwino, mzere wowongolera uyenera kuwoneka nthawi zonse.

Amapatsidwa ma reagents

Kiti iliyonse ili ndi:

Njira yodziwira

Werengani bukuli mosamala musanagwiritse ntchito kuti mupewe zotsatira zolakwika.

1. Musanayesedwe, ma reagents onse ayenera kukhala olingana ndi kutentha kwa chipinda (18 mpaka 25 ° C).

2. Chotsani khadi loyesera mu thumba la aluminiyamu zojambulazo ndikuziyika pamalo ophwanyika, owuma.

3. Gawo loyamba: Gwiritsani ntchito pipette kapena kusamutsa pipette kuti muwonjezere 10μL ya seramu/plasma, kapena 20μL ya chala chathunthu kapena magazi athunthu a venous pachitsimecho.

4. Khwerero 2: Nthawi yomweyo onjezerani madontho awiri (60µL) a bafa pachitsanzo.

5. Khwerero 3: Pamene mayesero ayamba kugwira ntchito, mukhoza kuona mtundu wofiira ukuyenda pawindo lomwe liri pakati pa khadi loyesa, ndipo zotsatira zoyesa zidzapezedwa mkati mwa mphindi 10-15..

Kutanthauzira zotsatira

Zabwino (+)

1. Pali mizere yofiira 3 (T1, T2, ndi C) muzenera la zomwe zimachitika.Ziribe kanthu kuti ndi mzere uti ukuwonekera koyamba, zikuwonetsa kukhalapo kwa ma antibodies atsopano a coronavirus IgM ndi IgG. 2. Pali mizere iwiri yofiira (T1 ndi C) pawindo la zomwe zimachitika, ziribe kanthu kuti mzere ukuwonekera poyamba, zimasonyeza kukhalapo kwa ma antibodies atsopano a coronavirus IgM. 3. Pali mizere iwiri yofiira (T2 ndi C) muzenera la zomwe zimachitika, ziribe kanthu kuti mzere ukuwonekera poyamba, zimasonyeza kukhalapo kwa ma antibodies atsopano a coronavirus IgG.

Zoipa(-)

1. Mzere wa "C" wokha (mzere wowongolera khalidwe) pazenera lazomwe umasonyeza kuti palibe ma antibodies ku coronavirus yatsopano yomwe yapezeka, ndipo zotsatira zake ndi zoipa.

Zosalondola

1. Ngati mzere wowongolera (C) sunawonetsedwe mkati mwa mphindi 10-15, zotsatira zoyesa ndizosavomerezeka mosasamala kanthu kuti pali T1 ndi / kapena T2 mzere.Ndikofunikira kuti muyesenso. 2. Zotsatira za mayeso ndizosavomerezeka pakatha mphindi khumi ndi zisanu.

Chifukwa chake mutha kuyesa kunyumba, imelo kapena kuyimba kuti mumve zambiri za Sars-CoV-2 IgM/IgG antibody kit (njira yagolide ya colloidal).