Chodziwika bwino cha machitidwe a PCR ndikukulitsa kwake kwakukulu komanso kukhudzidwa kwambiri.Kuti tiwongolere magwiridwe antchito a PCR ndikuzindikira bwino, tadzipereka kukulitsa mphamvu ya PCR yokulitsa komanso kuzindikira kuzindikira, koma mutu uli mkati moyesera.Zonama zabodza nthawi zambiri zimachitika, ndipo kuchepetsedwa kochepa kwambiri kwachitsanzo choyipitsidwa kapena kuipitsidwa kwazinthu za PCR kungayambitse zolakwika pakuyesa.
Mitundu isanu ya kuipitsidwa kwazinthu za PCR
Pali zifukwa zambiri zoyipitsira PCR, zomwe zitha kugawidwa m'magulu otsatirawa:
Kuipitsidwa kwa sampuli kumayambitsidwa makamaka ndi kuipitsidwa kwa chidebe chotengera chitsanzocho, kapena pamene chitsanzocho chayikidwa, chimatuluka mumtsuko chifukwa cha kusindikizidwa kosasunthika, kapena chitsanzocho chimamamatira kunja kwa chidebecho, zomwe zimayambitsa kuipitsidwa;Kuipitsidwa kumabweretsa kuipitsidwa pakati pa zitsanzo;Tizilombo tating'onoting'ono tating'ono, makamaka ma virus, amatha kufalikira ndi ma aerosols kapena kupanga ma aerosols, zomwe zimapangitsa kuipitsidwa.
Chifukwa chachikulu ndi chakuti panthawi yokonzekera PCR reagents, chitsanzo cha mfuti, chidebe, madzi osungunuka kawiri ndi njira zina zowonongeka ndi template ya PCR nucleic acid.
M'ma laboratories a mamolekyulu a biology ndi ma laboratories ena omwe amagwiritsa ntchito ma plasmid opangidwa ngati njira zabwino zowongolera, vuto la kuipitsidwa kwa plasmid lopangidwanso ndilofala.Chifukwa zomwe zili mu cloning plasmid mu voliyumu ya unit ndizokwera kwambiri, ndipo zida zambiri ndi ma reagents amafunikira pakuyeretsa, ndipo plasmid m'maselo amoyo ndizotheka kuipitsidwa chifukwa cha kukula kwakukulu ndi kubereka kwa maselo amoyo.
Kuyipitsidwa kwa zinthu zokulitsa ndiye vuto lodziwika bwino pakuyipitsidwa kwa PCR.Chifukwa kuchuluka kwa zinthu za PCR ndi zazikulu (zambiri 1013 makope/ml), zomwe ndi zapamwamba kwambiri kuposa malire a nambala yodziwikiratu ya PCR, kuipitsidwa kochepa kwambiri kwazinthu za PCR kumatha kuyambitsa zabodza.
Kuwonongeka kwa aerosol ndi njira yomwe ingawononge kwambiri zinthu za PCR, komanso ndiyosavuta kuinyalanyaza.Zimapangidwa ndi kukangana pakati pa madzi pamwamba ndi mpweya.Nthawi zambiri, kuipitsidwa kwa aerosol kumatha kupangidwa chivundikirocho chikatsegulidwa, chitsanzocho chikafunidwa, kapena ngakhale chubu chomwe chimagwedezeka chikugwedezeka mwamphamvu.Malinga ndi kuwerengera, tinthu tating'onoting'ono ta aerosol titha kukhala ndi makope 48,000, chifukwa chake kuipitsidwa komwe kumachitika chifukwa chake ndi vuto lomwe limayenera kusamala kwambiri.
Makamaka, ma laboratories oyesa nthawi zambiri amagwiritsa ntchito zoyambira ziwiri kuti ayese jini inayake.Pakapita nthawi, kuipitsidwa kwakukulu kwazinthu za PCR kudzachitika mu labotale.Kuipitsidwa kotereku kukachitika, kumakhala kovuta kuchotsa mu nthawi yochepa.
Mitundu itatu yoyambirira ya kuipitsidwa, titha kugwiritsa ntchito njira zabwino zopewera, koma kuipitsa komwe kumayambitsidwa ndi zinthu za PCR kumakhala kovuta kupewa, makamaka pomanga ma labotale a PCR omwe sali muyezo.Mu ndondomeko ya PCR, pamene nsonga ya pipette imayamwa ndikuwomba madzi, ndipo chivundikiro cha chubu cha PCR chimatsegulidwa, aerosol idzapangidwa.Mamolekyu a DNA otengedwa ndi aerosol (aerosol imodzi imatha kunyamula masauzande a DNA) ndi ovuta kuchotsa chifukwa amayandama mumlengalenga.Kamodzi kotsatira koyeserera kwa PCR kuyambika, zonena zabodza zidzachitika mosalephera.
Monga momwe zikuwonekera pachithunzichi, kuwongolera kolakwika kudakulitsanso gulu lofananira la chidwi:
Gawo loyamba la nkhani iyi ya PCR kuipitsa ndi kupewa kuyambitsidwa pano.Nkhani yotsatira idzakubweretserani gawo lachiwiri "Kupewa kuipitsidwa kwazinthu za PCR", choncho khalani maso!
Nthawi yotumiza: Jul-25-2017
