Kubadwa kwa PCR
PCR (Polymerase Chain Reaction)
Patha zaka zoposa 30 chiyambireni kupangidwa kwa polymerase chain reaction.Kwa zaka zoposa 30, akatswiri ambiri padziko lonse akupitiriza kuwonjezera ndi kusintha, teknoloji ya PCR yakhala njira yogwiritsidwa ntchito kwambiri komanso yofunikira kwambiri pa kafukufuku wa Life Sciences.
The TouchDown PCR, Real-Time PCR, Multi PCR, ndi zina zotero zopangidwa pamaziko a kugwiritsa ntchito ukadaulo wamba wa PCR, komanso Digital PCR (digital PCR) yomwe yangotuluka kumene, yalemeretsa kwambiri njira zofufuzira za ofufuza asayansi ambiri ndikufulumizitsa kwambiri chitukuko cha Sayansi ya Moyo wamakono, makamaka biology ya mamolekyulu, yathandizira kwambiri pa moyo wa anthu komanso chilengedwe.
Zolakwika zaukadaulo wakale wa PCR
Kupatukana kovutirapo kwa nucleic acid ndikuchotsa:
★ Zamakono PCR luso: chofunika
★ PCR yochokera luso: chofunika
★ Zitsanzo za DNA ndi RNA: kusiyana kwakukulu, zofunikira zogwirira ntchito zovuta
★ Zowopsa m'thupi: Zotulutsa poizoni zimawononga thupi
Ukadaulo wachikhalidwe wa PCR ndi ukadaulo wotumphukira uli ndi chofunikira-chofunikira-nucleic acid kulekana ndi kuyeretsedwa.
Zitsanzo zilizonse zachilengedwe zimayenera kudutsa muzosakaniza zovuta komanso zotopetsa kuti mupeze zitsanzo za nucleic acid zomwe zimakwaniritsa zofunikira zaukadaulo wa PCR.
Kulekanitsa ndi kuchotsa DNA ndi RNA nthawi zonse yakhala ntchito yofunikira yomwe akatswiri ofufuza asayansi ayenera kubwereza tsiku lililonse.
Chifukwa cha kusiyana kwakukulu pakati pa zitsanzo, kulekanitsa ndi kuchotsa njira za DNA ndi RNA ndizosiyana kwambiri.Ntchitoyi imafuna luso lapamwamba la luso la ogwira ntchito.Traditional kulekana ndi m'zigawo njira amafuna yaitali kukhudzana ndi ena kwambiri poizoni reagents mankhwala.Zimayambitsa kuwonongeka kosasinthika kwa thupi la wogwiritsa ntchitoyo, komanso kuwononga mwachindunji panthawi yoyesera.
Panthawi imodzimodziyo, kwa iwo omwe ali ndi zitsanzo zambiri zoti aphunzire, kulekanitsa ndi kuchotsa ma nucleic acid ndi ntchito yovuta kwambiri.
Nucleic acid kudzipatula ndi zida zotulutsa pamsika tsopano zakhwima ndipo pali mitundu yambiri, koma ndizofanana.Kaya ndi zida za silika gel membrane column centrifugal kit kapena njira ya maginito ya bead, zimatenga nthawi yambiri ndipo ndizokwera mtengo.Kuphatikiza pa mtengo wa zida, palinso zofunikira zapadera za zida za labotale.Malo ogwirira ntchito omwe amagwiritsidwa ntchito mu njira ya maginito ndi zida zamtengo wapatali kwambiri, zomwe zimakhala zodula kwambiri ku labotale.
Powombetsa mkota
Musanayambe kuyesa kwa PCR, kutengeka kwa zitsanzo ndizosapeweka ndipo nthawi zonse mutu wa mutu kwa ofufuza.Momwe mungathetsere vutoli komanso ngati kuyesa kwa PCR kungathe kuchitidwa popanda kupatukana ndi kutulutsa ma nucleic acid nthawi zonse kwakhala malingaliro a ofufuza ambiri asayansi ndi ogwira ntchito zachipatala.
Yankho la Foregene
Pambuyo pazaka zambiri za kafukufuku wovuta kwambiri paukadaulo wa Direct PCR ndi zida zofananira, Forgene adadutsa bwino m'mabotolo ambiri ndipo adakwanitsa bwino PCR yamitundu yambiri yamitundu yosiyanasiyana yokhala ndi kukana mwamphamvu komanso kusinthika, kulola ofufuza kuti achotse kulekanitsa kowopsa komanso kowopsa ndikuchotsa ma nucleic acid.Izi zidzachepetsa kwambiri kuchulukira kwa ntchito kwa aliyense, kufulumizitsa ntchito yoyesera, ndikupulumutsa ndalama zoyesa kafukufuku wasayansi.
Kumvetsetsa kwa Forgene ndi chidziwitso cha DirectPCR
Choyamba, ukadaulo wa DirectPCR ndiukadaulo wachindunji wa PCR wamitundu yosiyanasiyana yazachilengedwe.Pansi paukadaulo uwu, palibe chifukwa cholekanitsira ndikuchotsa ma nucleic acid, ndipo chitsanzo cha minofu chimagwiritsidwa ntchito mwachindunji ngati chinthucho, ndipo zoyambira za jini zimawonjezedwa pakuchita kwa PCR.
Chachiwiri, ukadaulo wa DirectPCR sikuti ndiukadaulo wokulirapo wa template wa DNA, komanso umaphatikizansopo RNA template reverse transcription PCR.
Chachitatu, ukadaulo wa DirectPCR sikuti umangochita molunjika pamachitidwe a PCR pazitsanzo za minofu, komanso umaphatikizanso zochitika za Real-Time qPCR, zomwe zimafuna kuti kachitidwe kachitidwe kake kakhale ndi mphamvu yolimba yolimbana ndi kusokoneza kwa fluorescence ndikutsutsa zozimitsa zoziziritsa kukhosi.
Chachinayi, zitsanzo za minofu zomwe zimagwiritsidwa ntchito ndi teknoloji ya DirectPCR zimangofuna kutulutsa ma templates a nucleic acid ndipo osachotsa mapuloteni, polysaccharides, ayoni amchere, ndi zina zomwe zimasokoneza PCR.Izi zimafuna nucleic acid polymerase ndi PCR Mix mumayendedwe kachitidwe kuti akhale ndi anti-reversibility ndi kusinthika, ndipo amatha kuwonetsetsa ntchito ya enzyme ndi kubwereza kulondola pamikhalidwe yovuta.
Chachisanu, zitsanzo za minofu zomwe zimayang'aniridwa ndi ukadaulo wa DirectPCR sizinapatsidwe chithandizo chilichonse chowonjezera cha nucleic acid, ndipo kuchuluka kwa ma template ndi kochepa kwambiri, komwe kumafunikira chidwi chachikulu komanso kukulitsa mphamvu zamachitidwe.
Mapeto
Tekinoloje ya DirectPCR ndi imodzi mwazinthu zofunikira kwambiri zaukadaulo komanso zatsopano m'zaka zapitazi za 30 kuyambira kubadwa kwaukadaulo wa PCR.Forgene ali ndipo apitiliza kukhala mpainiya komanso woyambitsa ukadaulo uwu.
Chiyembekezo chogwiritsa ntchito ukadaulo wa DirectPCR ndiwambiri.Kupititsa patsogolo ndi kupititsa patsogolo lusoli kudzabweretsa kusintha kosokoneza pa kafukufuku wa sayansi ndi ntchito yoyendera.Uku ndikusintha kwaukadaulo wa PCR.
Nthawi yotumiza: Feb-21-2017
