Khazikitsani kuyesa kwa PCR SOP kuti mukhazikitse machitidwe a anthu oyesera.
Woyesera amatsatira mosamalitsa njira zogwirira ntchito, ndipo amachepetsa kuipitsidwa kwa PCR komwe kumatha kuyambitsidwa ndi zinthu zaumunthu kapena kuletsa kuchitika kwa kuipitsa.Kuphatikiza apo, woyeserayo ayenera kukhala ndi chidziwitso ndi luso lofananirako, kuphatikiza luso lazida zogwirira ntchito, kumveketsa bwino ntchito yonse, kudziwa njira zochizira zoyipitsidwa ndi njira zowongolera zama labotale, ndikutha kutanthauzira molondola zotsatira za mayeso.
Khazikitsani labotale yokhazikika ya PCR.
PCR labotale lagawidwa m'madera anayi mfundo, kutanthauza reagent kukonzekera dera, chitsanzo processing dera, m'dera matalikidwe, ndi kukulitsa mankhwala kusanthula dera.Madera awiri oyambirira ndi malo okulitsa, ndipo madera awiri omalizira ndi malo opititsa patsogolo.Malo a pre-amplification ndi post-amplification zone ayenera kulekanitsidwa mosamalitsa.Zida zoyesera, ma reagents, mapepala ojambulira, zolembera, zipangizo zoyeretsera, ndi zina zotero, zimatha kuyenda kuchokera kumalo okulitsa chisanadze kupita ku malo opititsa patsogolo, ndiko kuti, kuchokera kumalo okonzekera reagent → dera lokonzekera chitsanzo → malo okulitsa → kusanthula kwazinthu zokulitsa, ndipo sayenera kuyenda chammbuyo .Mpweya wa mpweya mu labotale uyeneranso kuyenda kuchokera kumalo okulitsa chisanadze kupita kumalo opititsa patsogolo, osati kubwerera kumbuyo.Mapangidwe abwino a labotale ya PCR akuwonetsedwa pansipa:
Chithunzi A: Njira yabwino yokhazikitsira labotale ya PCR yokhala ndi kukakamiza koyipa m'chipinda cha buffer
Chithunzi B: Njira yabwino yokhazikitsira labotale ya PCR yokhala ndi kukakamiza kwabwino muchipinda chosungiramo
Zithunzi zokhazikitsira ma labotale a PCR zoperekedwa mu Chithunzi A ndi Chithunzi B ziyenera kukhala njira yabwino yokhazikitsira, ndipo labotale yokhala ndi mikhalidwe ingatanthauze mawonekedwe awa kuti apangidwe.Kwa ma laboratories wamba, tikulimbikitsidwa kuti malo okulitsa a PCR ndi malo owunikira zinthu zitha kupatulidwa, ndipo kutsegulidwa kwa chivundikirocho kuyenera kuchepetsedwa momwe kungathekere m'malo okonzekera zitsanzo ndi malo okulitsa a PCR.Kumbukirani: Zogulitsa ndi zoyesera zomwe zili m'malo owunikiridwa ndizoletsedwa kuti zitengedwere kumalo okonzekera zitsanzo ndi malo okulitsa a PCR.
Ngati labotale imangozindikira PCR ndikuzindikiritsa, tikulimbikitsidwa kugwiritsa ntchito fulorosenti ya PCR m'malo mwa PCR wamba.
Fluorescence kuchuluka kwa PCR kudziwika zotsatira akhoza kusonkhanitsidwa ndi kusanthula fluorescence siginecha, kotero palibe chifukwa kutsegula chivindikiro electrophoresis pambuyo anachita, amene amapewa PCR mankhwala kuipitsidwa chifukwa cha kutayikira mankhwala anachita kupanga aerosols.Ngati muwonjezera kuchuluka kwa mafungulo a kapu panthawi yokweza gel electrophoresis, kuipitsidwa kwa aerosol kumatha kuchitika.Ndi bwino kulimbikitsa ntchito kachulukidwe PCR ndi pang'onopang'ono m'malo khalidwe PCR.
Dongosolo la UNG anti-PCR loyipitsira zinthu limagwiritsidwa ntchito potengera PCR.
Dongosololi limagwiritsa ntchito dUTP m'malo mwa dTTP.Pambuyo pakuchita kwa PCR, zinthu zonse za PCR (zidutswa za DNA) zimaphatikizidwa ndi dUTP;mumzere wotsatira wa PCR reaction, puloteni ya UNG yowonjezeredwa ku dongosoloyi imayikidwa pa 37 ° C kwa mphindi 5 pamaso pa PCR, yomwe imatha kusokoneza mwapadera zidutswa zonse za DNA zomwe zili ndi dUTP, kenako kuchita PCR reaction.Izi zitha kuchotseratu kuipitsidwa kwa aerosol komwe kumayambitsidwa ndi zinthu za PCR.Zotsatira zake zikuwonetsedwa pachithunzichi:
Zindikirani: Kwa mndandanda wa PCR wachindunji, mutha kusankha zinthu zingapo za anti-PCR kuipitsidwa kwazinthu za Foregene.Lingalirani
Kwa ma laboratories omwe amayesa kwambiri ma genotyping, tikulimbikitsidwa kugwiritsa ntchito UNG anti-PCR product system poyesa ma reagents kuwonjezera pakupanga ma laboratories oyenera.
Chikumbutso: Kugwiritsa ntchito makinawa sikungachotse kuipitsidwa kwazinthu za PCR zomwe zayamba kale.Chifukwa chake, dongosolo la UNG liyenera kugwiritsidwa ntchito koyambirira kwa mayeso oyenera, ndipo dongosolo la UNG liyenera kugwiritsidwa ntchito pakukulitsa kwa PCR, kuti apewe kuipitsidwa kwa zinthu za PCR Zabodza.
Ndikofunikira kugwiritsa ntchito Direct PCR-UNG dongosolo la Foregene poyesa zazikulu, monga:
Plant Leaf Direct PCR Kit-UNG;
Plant Seed Direct PCR Kit-UNG;
Animal Tissue Direct PCR Kit-UNG;
Mouse Tail Direct PCR Kit-UNG;
Zebra Fish Direct PCR Kit-UNG.
Mndandanda wa zida za ForegeneSizingatheke kuzindikira PCR mwachangu komanso pamlingo waukulu, komanso kupewa ndikuwongolera kuipitsidwa kwazinthu za PCR.
Nthawi yotumiza: Mar-19-2021
