Kupanga SOP System

Khazikitsani kuyesa kwa PCR SOP kuti mukhazikitse machitidwe a anthu oyesera.

Oyeserawo amatsatira mosamalitsa njira zogwirira ntchito, ndikuchepetsa kuipitsidwa kwa PCR komwe kungayambike chifukwa cha anthu kapena kuletsa kuchitika kwa kuipitsa pakugwira ntchito.Kuphatikiza apo, woyeserayo ayenera kukhala ndi chidziwitso ndi luso lofananirako, kuphatikiza luso lazida zogwirira ntchito, kumveketsa bwino ntchito yonse, kudziwa njira zochizira zoyipitsidwa ndi njira zowongolera zama labotale, ndikutha kutanthauzira molondola zotsatira za mayeso.

Kumanga Standard PCR Laboratory

Laborator ya PCR imagawidwa m'magawo anayi, omwe ndi malo okonzekera reagent, malo opangira zitsanzo, malo okulitsa, ndi kusanthula kwazinthu zokulitsa.Madera awiri oyambirira ndi malo okulitsa, ndipo madera awiri omalizira ndi malo opititsa patsogolo.Malo a pre-amplification ndi post-amplification zone ayenera kulekanitsidwa mosamalitsa.zipangizo Experimental, reagents, kujambula pepala, zolembera, kuyeretsa zipangizo, etc. akhoza kungoyenda kuchokera m'dera chisanadze matalikidwe ku malo pambuyo matalikidwe, ndiko kuti, kuchokera reagent kukonzekera m'dera, dera processing chitsanzo, m'dera matalikidwe, ndi matalikidwe mankhwala kusanthula dera, ndipo sayenera kuyenda mu njira n'zosiyana.Mpweya wa mpweya mu labotale uyeneranso kuyenda kuchokera kumalo okulitsa chisanadze kupita ku malo okulitsa, osati mobwerera kumbuyo.

Chepetsani njira zoyesera

Ngati labotale imangozindikira PCR ndikuzindikiritsa, tikulimbikitsidwa kugwiritsa ntchito fulorosenti ya PCR m'malo mwa PCR wamba.

Fluorescence kuchuluka kwa PCR kudziwika zotsatira akhoza kusonkhanitsidwa ndi kusanthula fulorosenti siginecha, kotero palibe chifukwa kutsegula chivindikiro electrophoresis pambuyo anachita, amene amapewa kuipitsidwa PCR mankhwala chifukwa kutayikira mankhwala anachita kupanga aerosols.Ngati muwonjezera kuchuluka kwa mafungulo a kapu panthawi yokweza gel electrophoresis, kuipitsidwa kwa aerosol kumatha kuchitika.Ndi bwino kulimbikitsa ntchito kachulukidwe PCR ndi pang'onopang'ono m'malo khalidwe PCR.

Adopt UNG anti-pollution system

Dongosolo la UNG anti-PCR loyipitsira zinthu limagwiritsidwa ntchito potengera PCR.

Dongosololi limagwiritsa ntchito dUTP m'malo mwa dTTP.Pambuyo pakuchita kwa PCR, zinthu zonse za PCR (zidutswa za DNA) zimaphatikizidwa ndi dUTP;mumzere wotsatira wa PCR reaction, puloteni ya UNG yowonjezeredwa ku dongosoloyi imayikidwa pa 37 ° C kwa mphindi 5 pamaso pa PCR, yomwe ingakhale yeniyeni Kuwononga zidutswa zonse za DNA zomwe zili ndi dUTP, ndiyeno kuchita PCR.Izi zitha kuchotseratu kuipitsidwa kwa aerosol komwe kumayambitsidwa ndi zinthu za PCR.Zotsatira zake zikuwonetsedwa pachithunzichi:

Zindikirani: Pagulu lachindunji la PCR, mutha kusankha zinthu zingapo za anti-PCR zoipitsa dongosolo la FJ Biotech.

Lingalirani

Kwa ma laboratories omwe amayesa kwambiri ma genotyping, tikulimbikitsidwa kugwiritsa ntchito UNG anti-PCR product system poyesa ma reagents kuwonjezera pakupanga ma laboratories oyenera.

Chikumbutso: Kugwiritsa ntchito makinawa sikungachotse kuipitsidwa kwazinthu za PCR zomwe zayamba kale.Chifukwa chake, dongosolo la UNG liyenera kugwiritsidwa ntchito koyambirira kwa mayeso oyenera, ndipo dongosolo la UNG liyenera kugwiritsidwa ntchito nthawi zonse pakukulitsa PCR, kuti apewe kuipitsidwa kwa zinthu za PCR.Zonama zabodza.

Ndibwino kugwiritsa ntchito Direct PCR-UNG dongosolo la Forge Biotech poyesa zazikulu, monga:Plant Leaf Direct PCR Kit-UNG;

Plant Seed Direct PCR Kit-UNG;

Animal Tissue Direct PCR Kit-UNG;

Mouse Tail Direct PCR Kit-UNG;

Zebra Fish Direct PCR Kit-UNG.

Zida zotsatizanazi zochokera ku Forenene sizingangopanga kuzindikira kwa PCR mwachangu komanso pamlingo waukulu, komanso kupewa ndikuwongolera kuipitsidwa kwazinthu za PCR.