Chiyambi:

Mamolekyu a RNA amatha kuyanjana kwambiri ndi mamolekyu ena achilengedwe, monga mapuloteni, DNA ndi RNA, motero amakhala m'maselo ndi zamoyo monga ma macromolecular complexes.Pakati pawo, RNA-mapuloteni zovuta ndi kukhalapo ndi ntchito ya RNA Chimodzi mwazinthu zofunika kwambiri.Kutsitsa kwa RNA ndi njira yachikale yowunikira ndikuzindikira mamolekyu a RNA, makamaka mapuloteni omwe amalumikizana ndi mamolekyu a lncRNA, ndipo amadziwika ndi ofufuza ambiri komanso magazini ovomerezeka padziko lonse lapansi.Lero FORREGENE akugawana nanu mfundo yoyesera ndi njira yaukadaulo ya RNA kukokera pansi, mwina mutha kuyigwiritsa ntchito.

1. Mfundo yoyesera ya RNA ikugwetsa ukadaulo:

In vitro transcription and synthesis of biotin-labeled target lncRNA kapena lncRNA fragments (kuphatikizapo zidutswa za RNA zomwe zimagwiritsidwa ntchito ngati zowongolera zabwino ndi zoipa), ndi kuyika ndi zopangira maselo;gwiritsani ntchito mikanda ya maginito ya avidin kuti mutenge ma RNA-protein kapena RNA-RNA complexes, Mukatsuka bwino, zovutazo zimachotsedwa.Ngati zindikirani mapuloteni chandamale molekyulu kuti interacts ndi lncRNA, kupanga mapuloteni electrophoresis pa RNA-kukoka pansi mankhwala, kuzindikira chandamale mapuloteni ndi siliva madontho, ndi kudula gel osakaniza kwa misa spectrometry;komanso mwachindunji chitsimikiziro chakumadzulo kwa blot pa chinthu chokokera pansi;ngati zazindikirika Kwa mamolekyu omwe akutsata a RNA omwe amalumikizana ndi lncRNA, zinthu zokoka za RNA zimayikidwa m'zigawo za RNA ndipo kuzindikira kwa qRT-PCR kumachitika.

2. RNA ikugwetsa njira yoyesera luso:

3. Ntchito zaukadaulo za RNA kutsitsa FOREGENE:

Maselo chikhalidwe

Kukonzekera kwa maselo okwana / nyukiliya / mapuloteni a plasma

RNA ikugwetsa kuyesa

SDS-PAGE electrophoresis, banga lasiliva

Mass spectrometry ndi kuyesa kwa WB

Lipoti loyesera

4. Mlandu woyesera

Chithunzi chotsatira chikuwonetsa zotsatira za FJ Bio-Bio's RNA kutsika ndi kuzindikira kwa electrophoresis ya SDS-PAGE, momwe muvi wofiyira umawonetsa gulu losiyana:

Gulu lofufuza la Pulofesa Song Xu waku Sichuan University lapeza zaka 15 zakuchuluka kwa nthawi yayitali pantchito ya kafukufuku wa lncRNA.Gulu lofufuza la Foregene Bio ndi Pulofesa Song Xu adagwirizana kuti akhazikitse ukadaulo wa RNA wotsitsa wa lncRNA, womwe ungakupatseni zotsatira zokhutiritsa pakuyesa kwanu.