Pali mitundu iwiri ikuluikulu ya zoopsa mu labotale ya PCR: zoopsa za biosafety ndi ziwopsezo zoyipitsidwa ndi nucleic acid.Zakale zimavulaza anthu ndi chilengedwe, ndipo zotsirizirazi zimakhudza zotsatira za mayeso a PCR.Nkhaniyi ikunena za PCR labotale yowunikira zoopsa komanso milingo yofananira yomwe yabweretsedwa kwa inu.

01 Gawo la labotale ya PCR

1. Laboratory yoyezetsa mamolekyulu a biology

Malinga ndi zomwe zili mu Article 1.1 ya Basic Setting Standards for Clinical Gene Amplification Testing Laboratories, ma labotale a PCR nthawi zambiri amakhala ndi magawo anayi: malo osungiramo zinthu ndi malo okonzera, malo okonzera zitsanzo, malo okulitsa, ndi malo owunikira zinthu.Ngati njira yeniyeni ya fulorosenti ya PCR ikugwiritsidwa ntchito, malo okulitsa ndi malo owunikira akhoza kuphatikizidwa kudera limodzi;ngati PCR analyzer yokhazikika ikugwiritsidwa ntchito, malo okonzekera zitsanzo, malo okulitsa ndi malo owunikira akhoza kuphatikizidwa kudera limodzi.

"Workbook for New Coronavirus Nucleic Acid Testing in Medical Institutions (Trial Version 2)" imati makamaka, ma labotale omwe akuyesa ma coronavirus a nucleic acid akuyenera kukhazikitsa madera otsatirawa: malo osungiramo reagent ndi malo okonzekera, malo okonzera zitsanzo, kukulitsa ndi kusanthula zinthu.Madera atatuwa ayenera kukhala odziyimira pawokha popanda wina ndi mnzake mumlengalenga, ndipo sipangakhale kulumikizana kwachindunji ndi mpweya.

2. Chipinda chokonzekera chitsanzo

Ngakhale kuti zitsanzo zikhoza kukonzedwa m'malo okonzekera chitsanzo, chipinda chapadera chokonzekera chitsanzo chikufunikabe pochita ndi zitsanzo zovuta komanso zitsanzo zambiri.Chipinda chokonzekera chachitsanzo chili ndi chiopsezo chachikulu cha chitetezo chachilengedwe komanso kuipitsidwa kwa nucleic acid.

3. Chipinda chochizira zinyalala

Kusamalira zinyalala molakwika kudzabweretsanso chiopsezo chachikulu cha biosafety ndi kuipitsidwa kwa nucleic acid ku labotale.Choncho, chipinda chosungiramo zinyalala chiyenera kuyang'aniridwa nthawi zonse.

02 Malo owunikira zoopsa m'ma labotale a PCR

Ma laboratories osiyana amagawidwa m'chipinda chokonzekera zitsanzo, malo osungiramo reagent ndi malo okonzerako, malo okonzekera zitsanzo, malo okulitsa ndi kufufuza zinthu, ndi chipinda chosungiramo zinyalala.

Malinga ndi mtundu wa sampuli malo, amagawidwa pamwamba, chida, chitsanzo, kuwunika chilengedwe ndi pipette.

Mulingo wowopsa umayambira kutsika mpaka kumtunda kuchokera pa nyenyezi imodzi★ kufika pa nyenyezi zitatu★★★.

1. Zitsanzo zokonzekera chipinda:

Amagwiritsidwa ntchito polembetsa, kukonza ndi kuyambitsa zitsanzo, ndipo chiwopsezo chachitetezo chachilengedwe ndichokwera kwambiri.Chifukwa zitsanzo sizimachotsedwa ndikukulitsidwa, kupatula ma pipette omwe nthawi zambiri amakumana ndi zitsanzo, chiopsezo cha kuipitsidwa kwa nucleic acid m'madera ena ndi otsika.

1-4 Zitsanzo pa malo ounikira

5-8 Zitsanzo pamalo ounikira

9-12 mfundo zowunikira zitsanzo

1. Malo osungira ndi kukonzekera reagent:

Amagwiritsidwa ntchito pokonzekera zosungirako zosungirako, kugawa kwa ma reagents ndikukonzekera kusakaniza kwa amplification reaction, komanso kusungirako ndikukonzekera zinthu zomwe zimagwiritsidwa ntchito monga machubu a centrifuge ndi nsonga za pipette.Palibe kukhudzana mwachindunji ndi zitsanzo ndipo palibe nucleic acid yabwino m'derali, kotero chiopsezo cha biosafety ndi chiopsezo cha kuipitsidwa kwa nucleic acid ndizochepa.

13-16 Zitsanzo pa malo ounikira

17-22 Zitsanzo pakuwunika
3. Fotokozani mfundo zokonzera malo

Amagwiritsidwa ntchito potsegula mbiya yotengeramo, inactivate chitsanzo (pamene ikuyenera), kuchotsa nucleic acid ndikuwonjezera ku chubu cha amplification reaction, etc. Malowa angaphatikizepo kukonza ndi kutsegula zitsanzo, chiopsezo cha chitetezo chachilengedwe ndi chachikulu, ndipo nucleic acid m'zigawo ikuchitika, ndipo chiopsezo cha nucleic acid kuipitsidwa ndi sing'anga mpaka pamwamba.

29 Kutengera zitsanzo pamalo ounikira

4. Malo okulitsa ndi kusanthula malonda:

Amagwiritsidwa ntchito pakukulitsa kwa nucleic acid.Derali silimaphatikizapo kukonza zitsanzo, ndipo chiwopsezo chachitetezo chachilengedwe ndi chochepa.Ma nucleic acid amplification ali makamaka m'derali, ndipo chiopsezo cha kuipitsidwa kwa nucleic acid ndichokwera kwambiri.

38 Kutengera zitsanzo pamalo ounikira
5. Chipinda chosungiramo zinyalala:

Ntchito mkulu kuthamanga processing wa zitsanzo.Zowopsa zachitetezo chachilengedwe zomwe zimachitika pokonza zitsanzo m'derali ndizokwera kwambiri.Ma nucleic acid amplification amalangizidwa kuti azigwiritsidwa ntchito ngati zinyalala zachipatala.Kuthamanga kwakukulu sikuvomerezeka, ndipo chiopsezo cha kuipitsidwa kwa nucleic acid ndi chochepa.

43-44 Zitsanzo pa malo ounikira

03 Kukhazikitsa

Nthawi ino talemba 44 zowunikira.Akuti anthu ambiri amafunsa kuti, kodi amafunika kuchita zambiri?Inde, chitani zonse!Ndikukulangizani kuti muyambe kuyesa kuyesa kwa labotale yanu, yomwe ingachitike molingana ndi chiwopsezo kuchokera kumtunda kupita kumunsi, mutha kuyang'aniranso zitsanzo zamtundu womwewo palimodzi, kapena mutha kupanga dongosolo lachitsanzo lowunika pafupipafupi.Mwachidule, labotale iliyonse imatha kupanga ndondomeko yake yoyendetsera ntchito malinga ndi momwe zilili.Choopsa chachikulu pakuyesa ma laboratories ndikunyalanyaza zoopsa.