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Kupangidwa kwa injini ya nthunzi kunabweretsa kusintha kwa mafakitale, kupita patsogolo kwa makompyuta kunapanga dziko lamakono lamakono, ndipo kusintha kwaumisiri kudzabweretsadi kupita patsogolo kwa nthawi!Kubadwa kwaCell Direct RT-qPCR Kitya Foregene yatsala pang'ono kubweretsa nyengo yatsopano yozindikira mamolekyulu!

7min Pezani RNA template

Inde, mudamva bwino, osakayikira, kuchokera ku cell kupita ku RT-qPCR, tsopano masitepe awiri okha

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Lekani Kuchitapo kanthu

Q:Kodi kuchotsa RNA kukukwanira pa liwiro lothamanga chonchi?Kodi padzakhala kuipitsidwa kwa DNA?

A: Osadandaula, zogulitsa zathu zafananizidwa ndi kutsimikizika kosawerengeka, ndipo palibe kusiyana poyerekeza ndi zoyeserera zachikhalidwe za RT-qPCR.Kuphatikiza apo, kukula kwachitsanzo ndi kocheperako, ndipo kuyesako kumatha kuchitidwa ndi ma cell osachepera 10 otukuka.Kaya ndi mbale ya 6, 12, 24 kapena 96-chitsime, maselo amatha kuponyedwa mwachindunji m'zitsime za mbale, zomwe zimakhala zosavuta komanso zachangu.Ndi 5 × Direct RT Mix ndi 2 × Direct qPCR Mix muzogulitsa, template ya RNA ingagwiritsidwe ntchito kusinthika kwapamwamba komanso kukweza kwina, ndi kukhudzidwa kwakukulu, kukhazikika kwamphamvu komanso kukhazikika kwabwino.

Cell Direct RT-qPCR Kitsikuti ali ndi dongosolo lapamwamba la lysis, komanso ali ndi mphamvu yolimbana ndi RT-qPCR reaction inhibitors, zomwe zimapangitsa kuti zida zonse zikhale ndi malire otsika kwambiri, ndipo zimatha kupeza zotsatira zabwino pa maselo a 10.

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Nthawi yotumiza: Nov-26-2021