Cell Direct RT qPCR Kit

Kufotokozera Kwa Zida:

-Yosavuta komanso yothandiza: ndiukadaulo wa Cell Direct RT, zitsanzo za RNA zitha kupezeka m'mphindi 7 zokha.

-Zofuna zachitsanzo ndizochepa, zotsika ngati maselo a 10 akhoza kuyesedwa.

-Kutulutsa kwakukulu: imatha kuzindikira mwachangu RNA m'maselo opangidwa ndi mbale 384, 96, 24, 12, 6-chitsime.

-DNA Eraser imatha kuchotsa mwachangu ma genome omasulidwa, kuchepetsa kwambiri zotsatira zoyeserera.

-Optimized RT ndi qPCR system imapangitsa kuti RT-PCR reverse transcript ikhale yogwira mtima kwambiri komanso PCR yodziwika bwino, komanso yosagwirizana ndi RT-qPCR reaction inhibitors.

foregene strength


Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Kufotokozera

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer yomwe imatha kumasula RNA kuchokera ku zitsanzo zama cell otukuka pamachitidwe a RT-qPCR, potero amachotsa njira yoyeretsera ya RNA yomwe imatenga nthawi komanso yovuta.The RNA template ikhoza kupezeka mu mphindi 7 zokha.The 5×Direct RT Mix ndi 2×Direct qPCR Mix-SYBR reagents zoperekedwa ndi zida zitha kupeza mwachangu komanso moyenera zotsatira za PCR zenizeni zenizeni.

5×Direct RT Mix ndi 2×Direct qPCR Mix-SYBR imakhala ndi kulekerera kwamphamvu kwa inhibitor, ndipo ma lysate a zitsanzo amatha kugwiritsidwa ntchito ngati template ya RT-qPCR mwachindunji.Chidachi chili ndi RNA yapamwamba kwambiri ya Foregene reverse transcriptase, ndi Hot D-Taq DNA polymerase, dNTPs, MgCl.2, zotchingira zochita, PCR optimizer ndi stabilizer.

Zofotokozera

200×20μl Rxn, 1000×20μl rxns

Zida zamagulu

Gawo I

Mtengo CL

Foregene Protease Plus II

Mtengo wapatali wa magawo ST

Gawo II

DNA Eraser

5 × Direct RT Mix

2 × Mwachindunji qPCR Mix-SYBR

50 × ROX Reference Dye

RNase-Free ddH2O

Malangizo

Mbali&zabwino

Zosavuta komanso zothandiza : ndiukadaulo wa Cell Direct RT, zitsanzo za RNA zitha kupezeka m'mphindi 7 zokha.

■ Zofuna zachitsanzo ndizochepa, mpaka ma cell 10 akhoza kuyesedwa.

■ Kutulutsa kwakukulu: kumatha kuzindikira mwachangu RNA m'maselo opangidwa mu 384, 96, 24, 12, 6-chitsime mbale.

■ DNA Chofufutira akhoza kuchotsa mwamsanga matupi athu omasulidwa, kwambiri kuchepetsa zotsatira zoyeserera.

■ Makina okhathamiritsa a RT ndi qPCR amapangitsa kuti masitepe awiri a RT-PCR alembedwe bwino kwambiri komanso PCR yachindunji, komanso kugonjetsedwa ndi RT-qPCR reaction inhibitors.

Kit application

Kuchuluka kwa ntchito: maselo otukuka.

- RNA yotulutsidwa ndi zitsanzo za lysis: imagwira ntchito pa template ya RT-qPCR ya zida izi.

- Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kusanthula kwa jini, kutsimikizira kwa siRNA-mediated gene silence effect, kuyang'anira mankhwala, etc.

Chithunzi

Cell Direct RT qPCR diagram

Kusungirako ndi moyo wa alumali

Gawo I la zida izi ziyenera kusungidwa pa 4 ℃;Gawo II liyenera kusungidwa pa -20 ℃.

 Foregene Protease Plus II iyenera kusungidwa pa 4℃, osazizira pa -20 ℃.

 Reagent 2×Direct qPCR Mix-SYBR iyenera kusungidwa pa -20°Cmumdima;ngati imagwiritsidwa ntchito pafupipafupi, imathanso kusungidwa pa 4℃ posungira kwakanthawi kochepa (gwiritsani ntchito mkati mwa masiku 10).


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