Za RNA m'zigawo

Zaka makumi atatu zapitazo, chifukwa cha RNA m'zigawo, zomwe tingaganize zinali kokha kupeza RNA yathunthu, komabe, panalibe kufunikira kwa kuthamanga kwa m'zigawo (M'badwo woyamba wa Techology njira ya Trizol yobadwa).Ndi kusinthika kobwerezabwereza kwaukadaulo wochotsa RNA, zomwe tingachite sizongopeza zotsatira.Pamene anayang'anizana ndi m'zigawo za m'badwo woyamba Trizol ofotokoza reagents, experimenters zambiri ankavutika ndi masitepe ake zovuta ntchito;ngakhale zitatha kukhathamiritsa ndi kukonzanso zambiri, zidatenga nthawi yayitali.Nthawi yomweyo, ma reagents organic monga phenol ndi chloroform ayenera kugwiritsidwa ntchito pochotsa, zomwe zimawopsezanso chitetezo cha ogwira ntchito kumlingo wina.

Zothandiza, zosavuta komanso zotetezeka

Kupeza chothandizira, chosavuta komanso chotetezeka cha RNA chakhala cholinga chachikulu cha oyesera.Chifukwa chake, njira yotsatsira ya Silica Spin Column yochotsa RNA idayamba kukhalapo.Foregene wa m'badwo wachitatu RNA m'zigawo mankhwala amatengera ubwino wa m'badwo wam'mbuyo ndime m'zigawo mankhwala, pogwiritsa ntchito (DNA-Kuyeretsa + RNA yekha) awiri Spin Column luso kuchotsa gDNA ndi zinyalala minofu pa nthawi yomweyo, popanda DNase mankhwala otsala gDNA, ndipo osagwiritsanso ntchito phenol, etc. Poizoni ndi zovulaza reagents, purity yaing'ono ndi kuwononga reagents, nthawi yaifupi, purity ndi zokolola zochepa, kuti akwaniritse zokolola zapang'onopang'ono.

Tchati chofananira cha ntchito

Tchati choyerekeza zotsatira

Foregene total RNA isolation kit imakhala ndi zokolola zambiri za RNA, kuyera bwino, komanso kutulutsa kwa miRNA ndizabwinoko kuposa zida zina zamtundu.

Electrophoresis ya 50 mg ya mbewa chiwindi okwana RNA yotengedwa ndi njira zosiyanasiyana
M'badwo woyamba (1: Trizol)
M'badwo Wachiwiri (2: Zida za RNA kuchokera ku kampani A)
M'badwo wachitatu (3: zida zotulutsa RNA kuphatikiza kuchokera ku kampani A, 4: Foregene:RNA Isolation kits)

qPCR graph (kukulitsa curve graph)

Genomic DNA iyenera kuchotsedwa bwino panthawi yochotsa RNA, apo ayi izo zidzakhala ndi zotsatira zosalamulirika pa zoyesera zapansi (mtengo woyambirira wa CT, zonyenga zabodza).Zida zam'badwo wotsatira zimatha kuchotsa bwino ma genomic DNA popanda kuwonjezera DNase, kupulumutsa nthawi yambiri, ndikupewa kuwonongeka komwe kumachitika chifukwa cha kuipitsidwa kwa RNase.
M'badwo woyamba (zobiriwira zimasonyeza TRlzol-, zofiira zimasonyeza TRlzol +)

M'badwo wachiwiri (Buluu akuwonetsa zida za Company A RNA m'zigawo -, zofiira zikuwonetsa zida za Company A RNA +)

M'badwo wachitatu (buluu limasonyeza kampani A RNA m'zigawo zida kuphatikiza, zobiriwira zimasonyeza Foregene RNA Isolation zida)

(Dziwani: “_” pa graph ya qPCR ikusonyeza kuti DNase sinawonjezedwe, ndipo kuipitsidwa kwa DNA sikunachotsedwe; “+” akusonyeza kuti DNase yawonjezedwa, ndipo kuipitsidwa kwa DNA kwachotsedwa)

Malizitsani kuchotsa RNA mkati mwa 11min

Palibe chifukwa chazaka zambiri zakuchulukira kwa nucleic acid m'zigawo, Foregene mkulu-kokwanira kwathunthu RNA kudzipatula zida zotsatizana zitha kukulolani kuti mupeze zokolola zambiri komanso zoyera kwambiri za RNA mkati mwa mphindi 11 kuti mukwaniritse zosowa zosiyanasiyana zoyesera.

Mpaka pano, Foregene total RNA isolaton kits series product zadziwika ndi makasitomala ambiri ndipo zathandizira zolemba zambiri zapamwamba.Makasitomala akugwiritsa ntchito tchati cha electrophoresis komanso kuchuluka kwa PCR amplification pamapindikira pambuyo polemba mosinthana zikuwonetsa kuti RNA yotengedwa ndikugwiritsa ntchito zinthu za Foregene sikuti imangoyang'ana, komanso kukhulupirika bwino.Zida zamakono zokha zomwe zayesedwa ndi msika zikhoza kukondedwa ndi aliyense.

Cell Total RNA Isolation Kit

Mtengo wapatali wa magawo RNA

Fast: Malizitsani Kudzipatula kwa RNA mu mphindi 11

Chitetezo: Palibe mankhwala achilengedwe omwe amawonjezeredwa

Zolemba zina zogoletsa kwambiri:

1. Yuan Fang, Zezhong Liu, Yang Qiu, et al.Kuletsa kwa ma virus suppressor a mapuloteni a RNAi ndi ma peptides opanga kumateteza ku matenda a enteroviral mu vivo, Immunity, 54(10), 2021: 2231-2244.e6.( NGATI: 31.745,Viral RNA Isolation Kit,Cat No.RE-02011)

https://www.sciencedirect.com/science/article/pii/S1074761321003617

2. Ren, Y., Wang, A., Wu, D. et al.Kuletsa kwapawiri kwa chitetezo cham'mimba ndi apoptosis ndi mapuloteni amtundu wa cytomegalovirus UL37x1 kumathandizira kuti ma virus abwere.Nat Microbiol 7, 1041-1053 (2022).(IF:30.964,Cell Total RNA Isolation Kit,Cat No.RE-03111)

https://doi.org/10.1038/s41564-022-01136-6