Animal Total RNA Isolation Kit

Kufotokozera Kwa Zida:

Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.Dongosolo lonse ndi RNase-Free

Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column

Chotsani DNA popanda kuwonjezera DNase

Zosavuta-zochita zonse zimatsirizidwa pa kutentha kwa chipinda

Kuthamanga-ntchito ikhoza kutha m'mphindi 30

Otetezeka-palibe organic reagent yomwe imagwiritsidwa ntchito

Kuyera kwakukulu-OD260/280≈1.8-2.1

foregene strength


Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Mafotokozedwe a Kits

50 Preps, 200 Preps

Chidachi chimagwiritsa ntchito spin column ndi formula yopangidwa ndi kampani yathu, yomwe imatha kutulutsa RNA yoyera komanso yapamwamba kwambiri kuchokera kumagulu osiyanasiyana anyama ndikuchita bwino kwambiri. kuchokera ku supernatant ndi minofu lysate, yosavuta komanso yopulumutsa nthawi;Mzere wa RNA wokha ukhoza kumangirira RNA bwino ndipo ukhoza kusinthidwa nthawi imodzi ndi mitundu yapadera ya zitsanzo zambiri.

Dongosolo lonselo ndi RNase-Free, kotero kuti RNA yochotsedwayo isawonongeke;Buffer RW1, Buffer RW2 buffer makina ochapira, kotero kuti RNA yopezekayo ilibe mapuloteni, DNA, ion, ndi kuipitsidwa kwapawiri.

 

Zida za zida:

Buffer RL1 , Buffer RL2
Buffer RW1, Buffer RW2
RNase-Free ddH2O, Gawo Loyeretsa DNA
RNA-Only Column

Mbali&zabwino

■ Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA;dongosolo lonse ndi RNase-Free
■ Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column
■ Chotsani DNA popanda kuwonjezera DNase
■ Zosavuta-zochita zonse zimamalizidwa ndi kutentha kwa firiji
■ Kuthamanga kwachangu kumatha kutha mphindi 30
■ Safe-palibe organic reagent chofunika
■ Kuyera kwakukulu -OD260/280≈1.8-2.1

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Kit application

Ndiwoyenera kuchotsedwa ndi kuyeretsedwa kwa RNA yonse kuchokera kumitundu yosiyanasiyana yanyama yatsopano kapena yozizira kapena maselo otukuka.

Mankhwala magawo

■ Ntchito zotsika: kaphatikizidwe ka cDNA yoyamba, RT-PCR, kupanga maselo, Northern Blot, etc.
■ Zitsanzo: minofu ya nyama, maselo otukuka
■ Mlingo: Tissues 10-20mg, Maselo(2-5)×106
■ Kuchuluka kwa DNA kumangiriza mphamvu yoyeretsa: 80 μg
■ Voliyumu yowonjezera: 50-200 μl

Kuyenda kwa ntchito

animal total RNA-simple workflow

Chithunzi

3 Animal Total RNA Isolation Kit7

Animal Total RNA Isolation Kit ankachitira 20mg
Zitsanzo za mbewa zatsopano, tengani 5% oyeretsedwa okwana RNA 1% agar

Glycogel electrophoresis
1: Nkhumba 2: Impso
3: Chiwindi 4: Mtima

Kusungirako ndi moyo wa alumali

Zida zimatha kusungidwa kwa miyezi 24 kutentha kwapakati (15-25 ℃) kapena 2-8 ℃ kwa nthawi yayitali.Buffer RL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi wa 1 mutawonjezera β- mercaptoethanol (posankha).


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