Ndife odziwa kupanga.Kupambana ambiri paziphaso zofunika kwambiri pamsika wake wa Mtengo Wotsika mtengo China Lab 64 Well Plate Magnetic Bead Nucleic Acid ExtractionDNA Rna Purification Isolation Kitndi CE, Pamodzi ndi khama lathu, mankhwala athu anapambana chikhulupiriro cha makasitomala ndipo akhala salable kwambiri kuno ndi kunja.
Ndife odziwa kupanga.Kupambana ambiri kuchokera kuzitsimikizo zofunika za msika wakeChina Nucleic Acid m'zigawo zida, DNA Rna Purification Isolation Kit, Tikufuna kuitana makasitomala ochokera kunja kudzakambirana nafe zamalonda.Titha kupereka makasitomala athu zinthu zapamwamba komanso ntchito yabwino kwambiri.Ndife otsimikiza kuti tidzakhala ndi ubale wabwino ndikukhala ndi tsogolo labwino kwa onse awiri.

Zofotokozera

50 Preps, 200 Preps

Chidacho chimagwiritsa ntchito spin column ndi formula yopangidwa ndi Foregene, yomwe imatha kuchotsa bwino RNA yoyera komanso yapamwamba kwambiri kuchokera kumitundu yosiyanasiyana yazomera yokhala ndi ma polysaccharides otsika ndi ma polyphenols.Pazitsanzo za zomera zokhala ndi ma polysaccharides apamwamba kapena ma polyphenols, tikulimbikitsidwa kugwiritsa ntchito Plant Total RNA Isolation Plus Kit kuti mupeze zotsatira zabwinoko zochotsa RNA.Chidachi chimapereka DNA-Cleaning column yomwe imatha kuchotsa mosavuta DNA ya genomic kuchokera ku supernatant ndi minofu lysate.Mzere wa RNA wokha ukhoza kumangirira RNA.Chidachi chimatha kukonza zitsanzo zambiri nthawi imodzi.

Dongosolo lonse lilibe RNase, kotero RNA yoyeretsedwa sidzanyozedwa.Buffer PRW1 ndi Buffer PRW2 akhoza kuonetsetsa kuti RNA yopezedwa siipitsidwa ndi mapuloteni, DNA, ayoni, ndi organic compounds.

Zida zamagulu

Mbali & ubwino

■ Opaleshoni firiji (15-25 ℃) lonse ndondomeko, popanda ayezi kusamba ndi otsika kutentha centrifugation.
■ Zida zonse za RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
■ DNA-Cleaning Column imamangiriza ku DNA, kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
■ Zokolola zambiri za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
■ Kuthamanga kwachangu: kosavuta kugwira ntchito ndipo kumatha kutha mkati mwa mphindi 30.
■ Chitetezo: palibe organic reagent yomwe imafunikira.
■ Ubwino wapamwamba: Zidutswa za RNA zoyeretsedwa ndi zoyera kwambiri, zopanda zomanga thupi ndi zonyansa zina, ndipo zimatha kukumana ndi zoyeserera zosiyanasiyana zakutsika.

Kit application

Ndiwoyenera kuchotsa ndi kuyeretsa RNA yonse kuchokera ku zitsanzo zamafuta a zomera zatsopano kapena zozizira (makamaka masamba atsopano a masamba) okhala ndi polysaccharide yochepa ndi polyphenol.

Kuyenda kwa ntchito

Chithunzi

Plant Total RNA Isolation Kit Plus inakonzedwa 50mg masamba atsopano a polysaccharides ndi polyphenols, ndipo 5% yoyeretsedwa RNA inayesedwa ndi electrophoresis.
1: nthochi
2: Chiko
3: thonje
4: Khangaza

Kusungirako ndi moyo wa alumali

Zida zimatha kusungidwa kwa miyezi 12 kutentha kwa firiji (15-25 ℃) pamalo owuma, ndi 2-8 ℃ kwa nthawi yayitali (miyezi 24).

Buffer PSL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi umodzi mutawonjezera 2-hydroxy-1-ethanethiol (ngati mukufuna). Ndife odziwa kupanga.Kupambana ambiri paziphaso zofunika kwambiri pamsika wake wa Mtengo Wotsika mtengo China Lab 64 Chabwino Plate Magnetic Bead Nucleic Acid Extraction DNA Rna Purification Isolation Kit ndi CE, Pamodzi ndi khama lathu, malonda athu apambana chidaliro chamakasitomala ndipo akhala akugulitsidwa kwambiri kuno ndi kunja.
Mtengo Wotsika mtengoChina Nucleic Acid m'zigawo zida, DNA Rna Purification Isolation Kit, Tikufuna kuitana makasitomala ochokera kunja kudzakambirana nafe bizinesi.Titha kupereka makasitomala athu zinthu zapamwamba komanso ntchito yabwino kwambiri.Ndife otsimikiza kuti tidzakhala ndi ubale wabwino ndikukhala ndi tsogolo labwino kwa onse awiri.