Tili ndi gulu lochita bwino lomwe kuti lithane ndi mafunso kuchokera kwa omwe akuyembekezeka.Cholinga chathu ndi "100% kukwaniritsidwa kwamakasitomala ndi zinthu zathu zabwino kwambiri, mtengo & gulu lathu" ndikusangalala ndi mbiri yabwino kwambiri pakati pa makasitomala.Ndi mafakitale ambiri, titha kupereka mitundu yambiri ya China Mtengo Wotsika mtengo China 100t/Kit Extraction-Free Nucleic Acid Release Reagent for Genetic PCR Detection, Mamembala athu ali ndi cholinga chopereka malonda ndi chiwongola dzanja chamtengo wapatali kwa ogula athu, komanso cholinga cha tonsefe nthawi zambiri chimakhala kukhutiritsa ogula athu ochokera kuzungulira chilengedwe.
Tili ndi gulu lochita bwino lomwe kuti lithane ndi mafunso kuchokera kwa omwe akuyembekezeka.Cholinga chathu ndi "100% kukwaniritsidwa kwamakasitomala ndi zinthu zathu zabwino kwambiri, mtengo & gulu lathu" ndikusangalala ndi mbiri yabwino kwambiri pakati pa makasitomala.Ndi mafakitale ambiri, titha kupulumutsa mosavuta mitundu yosiyanasiyanaChina DNA Rna Release Reagent, DNA Release Reagent, Onetsetsani kuti ndinu omasuka kutitumizira zomwe mukufuna ndipo tikuyankhani posachedwa.Tsopano tili ndi gulu laukadaulo laukadaulo kuti likuthandizireni pazosowa zanu zonse.Zitsanzo zopanda mtengo zitha kutumizidwa kuti zigwirizane ndi zosowa zanu kuti mumvetsetse zambiri.Kuti mukwaniritse zomwe mukufuna, onetsetsani kuti mwamasuka kulumikizana nafe.Mutha kutitumizira maimelo ndikulumikizana nafe mwachindunji.Komanso, timalandira alendo ku fakitale yathu padziko lonse lapansi kuti azindikire bwino gulu lathu.ndi zinthu.Pochita malonda athu ndi amalonda a mayiko ambiri, nthawi zambiri timatsatira mfundo yofanana ndi kupindula.Ndi chiyembekezo chathu kugulitsa, mwa kuyesetsa limodzi, malonda ndi ubwenzi uliwonse kuti tipindule.Tikuyembekezera kupeza mafunso anu.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo

■ Kufuna kwachitsanzo ndikochepa, monga momwe minofu yanyama ya 5mg ingayesedwe.

■ Palibe chithandizo chapadera monga kugaya ndi kuphwanya chofunika, ndipo opaleshoniyo ndi yosavuta.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

■ Anti-kuipitsa PCR dongosolo 2 × PCR Easy^TMMix (UNG) imathetsa bwino kuipitsidwa kwa zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Zosintha za Kit

Ntchito: zosiyanasiyana nyama zimakhala.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer AL ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

Reagent Foregene Protease ili ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

Reagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

Reagent 2 × PCR Yosavuta^TMMix(UNG), ngati imagwiritsidwa ntchito pafupipafupi, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (igwiritsidwe ntchito mkati mwa masiku 10) .Tili ndi gulu lochita bwino kwambiri lothana ndi mafunso ochokera kwa omwe akuyembekezeka.Cholinga chathu ndi "100% kukwaniritsidwa kwamakasitomala ndi zinthu zathu zabwino kwambiri, mtengo & gulu lathu" ndikusangalala ndi mbiri yabwino kwambiri pakati pa makasitomala.Ndi mafakitale ambiri, titha kupereka mitundu yambiri ya China Mtengo Wotsika mtengo China 100t/Kit Extraction-Free Nucleic Acid Release Reagent for Genetic PCR Detection, Mamembala athu ali ndi cholinga chopereka malonda ndi chiwongola dzanja chamtengo wapatali kwa ogula athu, komanso cholinga cha tonsefe nthawi zambiri chimakhala kukhutiritsa ogula athu ochokera kuzungulira chilengedwe.
China Cheap mtengoChina DNA Rna Release Reagent, DNA Release Reagent, Onetsetsani kuti ndinu omasuka kutitumizira zomwe mukufuna ndipo tikuyankhani posachedwa.Tsopano tili ndi gulu laukadaulo laukadaulo kuti likuthandizireni pazosowa zanu zonse.Zitsanzo zopanda mtengo zitha kutumizidwa kuti zigwirizane ndi zosowa zanu kuti mumvetsetse zambiri.Kuti mukwaniritse zomwe mukufuna, onetsetsani kuti mwamasuka kulumikizana nafe.Mutha kutitumizira maimelo ndikulumikizana nafe mwachindunji.Komanso, timalandira alendo ku fakitale yathu padziko lonse lapansi kuti azindikire bwino gulu lathu.ndi zinthu.Pochita malonda athu ndi amalonda a mayiko ambiri, nthawi zambiri timatsatira mfundo yofanana ndi kupindula.Ndi chiyembekezo chathu kugulitsa, mwa kuyesetsa limodzi, malonda ndi ubwenzi uliwonse kuti tipindule.Tikuyembekezera kupeza mafunso anu.