Kampaniyo imapitilira lingaliro la "kasamalidwe ka sayansi, luso lapamwamba komanso luso lapamwamba, wogula wamkulu wa China Manufacturer wa 2 × Concentrated Premix for Unpurified Sample Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Bizinesi yathu idadzipereka kupatsa makasitomala zinthu zofunika kwambiri komanso zotetezeka zamakasitomala pamtengo uliwonse waukali komanso wankhanza.
Kampaniyo imasunga lingaliro la "kasamalidwe ka sayansi, luso lapamwamba komanso luso lapamwamba, wogula wamkuluChina Taq DNA Polymerase ndi Qpcr, Kampani yathu ili ndi mphamvu zambiri ndipo ili ndi njira yokhazikika komanso yabwino yogulitsira malonda.Tikulakalaka titha kukhazikitsa ubale wabwino wamabizinesi ndi makasitomala onse ochokera kunyumba ndi kunja pamaziko a zopindulitsa zonse.
Real Time PCR primer design mfundo

Forward Primer ndi Reverse Primer

Kwa Real Time PCR, mapangidwe oyambira ndi ofunika kwambiri.Zoyambira zimayenderana ndi kutsimikizika komanso kuthekera kwa kukulitsa kwa PCR, ndipo zitha kupangidwa motengera mfundo izi:

• Kutalika koyambirira: 18-30bp.
• Zolemba za GC: 40-60%.
• Mtengo wa Tm: Mapulogalamu opangira ma primer, monga Primer 5, amatha kupereka mtengo wa Tm woyambira.Makhalidwe a Tm oyambira kumtunda ndi kumtunda ayenera kukhala pafupi momwe angathere.Njira yowerengera ya Tm ingagwiritsidwenso ntchito: Tm = 4 °C (G + C) + 2 °C (A + T).Pochita PCR, kutentha pansi pa mtengo woyambira wa Tm wa 5 ° C nthawi zambiri kumasankhidwa ngati kutentha kwapang'onopang'ono (kuwonjezeka kofananirako kwa kutentha kwapang'onopang'ono kumatha kukulitsa momwe PCR imachitira).
• Primers ndi zinthu za PCR:

1. Design primer PCR amplification product kutalika makamaka 100-150bp.
2. Zoyambira zojambula m'dera lachiwiri la template ziyenera kupewedwa momwe zingathere.
3. Pewani kupanga zoyambira ziwiri kapena kupitilira apo pakati pa 3′ malekezero oyambira kumtunda ndi kumunsi kwa mtsinje.
4. Primer 3′ terminal base sangakhalepo ndi 3 zowonjezera zotsatizana za G kapena C.
5. Zoyambira zokha sizingakhale ndi zida zowonjezera, apo ayi mawonekedwe atsitsi adzapangidwa, zomwe zimakhudza kukulitsa kwa PCR.
6. ATCG iyenera kugawidwa mofanana momwe mungathere poyambira, ndipo 3′ terminal base iyenera kupewedwa ngati T.

Zowonjezera1:Cndi DirectRT-qPCR Kit chigawot paketi yowonjezera

1.Cell Lysis Solution

 Kampaniyo imapitilira lingaliro la "kasamalidwe ka sayansi, luso lapamwamba komanso luso lapamwamba, wogula wamkulu wa China Manufacturer wa 2 × Concentrated Premix for Unpurified Sample Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Bizinesi yathu idadzipereka kupatsa makasitomala zinthu zofunika kwambiri komanso zotetezeka zamakasitomala pamtengo uliwonse waukali komanso wankhanza.
China wopanga kwaChina Taq DNA Polymerase ndi Qpcr, Kampani yathu ili ndi mphamvu zambiri ndipo ili ndi njira yokhazikika komanso yabwino yogulitsira malonda.Tikulakalaka titha kukhazikitsa ubale wabwino wamabizinesi ndi makasitomala onse ochokera kunyumba ndi kunja pamaziko a zopindulitsa zonse.

Real Time PCR primer design mfundo

Forward Primer ndi Reverse Primer

Kwa Real Time PCR, mapangidwe oyambira ndi ofunika kwambiri.Zoyambira zimayenderana ndi kutsimikizika komanso kuthekera kwa kukulitsa kwa PCR, ndipo zitha kupangidwa motengera mfundo izi:

• Kutalika koyambirira: 18-30bp.
• Zolemba za GC: 40-60%.
• Mtengo wa Tm: Mapulogalamu opangira ma primer, monga Primer 5, amatha kupereka mtengo wa Tm woyambira.Makhalidwe a Tm oyambira kumtunda ndi kumtunda ayenera kukhala pafupi momwe angathere.Njira yowerengera ya Tm ingagwiritsidwenso ntchito: Tm = 4 °C (G + C) + 2 °C (A + T).Pochita PCR, kutentha pansi pa mtengo woyambira wa Tm wa 5 ° C nthawi zambiri kumasankhidwa ngati kutentha kwapang'onopang'ono (kuwonjezeka kofananirako kwa kutentha kwapang'onopang'ono kumatha kukulitsa momwe PCR imachitira).
• Primers ndi zinthu za PCR:

1. Design primer PCR amplification product kutalika makamaka 100-150bp.
2. Zoyambira zojambula m'dera lachiwiri la template ziyenera kupewedwa momwe zingathere.
3. Pewani kupanga zoyambira ziwiri kapena kupitilira apo pakati pa 3′ malekezero oyambira kumtunda ndi kumunsi kwa mtsinje.
4. Primer 3′ terminal base sangakhalepo ndi 3 zowonjezera zotsatizana za G kapena C.
5. Zoyambira zokha sizingakhale ndi zida zowonjezera, apo ayi mawonekedwe atsitsi adzapangidwa, zomwe zimakhudza kukulitsa kwa PCR.
6. ATCG iyenera kugawidwa mofanana momwe mungathere poyambira, ndipo 3′ terminal base iyenera kupewedwa ngati T.

Zowonjezera1:Cndi DirectRT-qPCR Kit chigawot paketi yowonjezera

1.Cell Lysis Solution

Mabuku a Malangizo:

 Quick Easy Cell Direct RT-qPCR Kit-Taqman