China China Yogulitsa China Zonse Nyengo Yabwino Kwambiri Yotsika mtengo kwambiri PCR wopanga ndi katundu |Foregene

China Yogulitsa China Yonse Nyengo Yabwino Kwambiri Yotsika mtengo-Yogwira Ntchito PCR

China Yogulitsa China Yonse Nyengo Yabwino Kwambiri Yotsika mtengo-Yogwira Ntchito pa PCR Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwachangu DNA ya genomic kuchokera ku zebrafish ndi minofu ina yamadzi am'madzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuchita kwa PCR, kotero ndizoyenera kuyesa kwakukulu kwa majini.Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65 ° C.Palibe njira zina monga kuchotsedwa kwa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2 × PCR Easy TM Mix ili ndi kulekerera kwamphamvu kwa PCR reaction inhibitors, ndipo ingagwiritse ntchito lysate ya chitsanzo kuti iyesedwe ngati template kuti ikhale yowonjezereka komanso yowonjezereka.Reagent iyi ili ndi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, buffer reaction, PCR optimizer ndi stabilizer.Kugwiritsidwa ntchito molumikizana ndi chotchinga cha lysis kumatha kuzindikira mwachangu komanso mosavuta zitsanzo, ndipo kumakhala ndi mawonekedwe amphamvu kwambiri, kukhazikika kwamphamvu, komanso kukhazikika bwino.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Chinsinsi cha kupambana kwathu ndi "Good Product Quality, Value Value and Efficient Service" for China Wholesale China All Season Best Quality Cost-Effective PCR, "Quality", "kuona mtima" ndi "service" ndi mfundo yathu.Kukhulupirika kwathu ndi kudzipereka kwathu kumakhalabe mwaulemu pantchito yanu.Lankhulani nafe Lero Kuti mudziwe zambiri, lemberani ife tsopano.
Chinsinsi cha kupambana kwathu ndi "Good Product Quality, Phindu Loyenera ndi Ntchito Yogwira Ntchito" yaPCR Yabwino Kwambiri, China All Season PCR, Gulu lathu limadziwa bwino zomwe msika umafuna m'maiko osiyanasiyana, ndipo limatha kupereka zinthu zabwino pamitengo yabwino kumisika yosiyanasiyana.Kampani yathu yakhazikitsa kale gulu loyenerera, lopanga komanso lodalirika kuti litukule makasitomala ndi mfundo zopambana zambiri.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	FP yoyipa
	Foregene Protease
	6 × DNA Loading Buffer
Gawo II	2 × PCR Yosavuta^TMSakanizani
Malangizo

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

■ Zofuna zachitsanzo ndizochepa, monga 1mg nsomba ya caudal fin kapena mazira 10 a nsomba angayesedwe.

■ Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo opaleshoniyo ndi yosavuta.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Zosintha za Kit

Kugwiritsa ntchito: Nsomba za Zebra ndi nsomba zina zam'madzi.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

wk-flw-zebra-fish-direct-PCR

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer FP ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

v Reagent Foregene Protease ali ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

vReagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

vReagent 2 × PCR EasyTM Mix, ngati imagwiritsidwa ntchito kawirikawiri, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (ntchito mkati mwa masiku 10).

Chinsinsi cha kupambana kwathu ndi "Good Product Quality, Value Value and Efficient Service" for China Wholesale China All Season Best Quality Cost-Effective PCR 185/70r13, "Quality", "kuona mtima" ndi "service" ndi mfundo yathu.Kukhulupirika kwathu ndi kudzipereka kwathu kumakhalabe mwaulemu pantchito yanu.Lankhulani nafe Lero Kuti mudziwe zambiri, lemberani ife tsopano.
China Wholesale China All Season PCR, Best Quality PCR, gulu lathu limadziwa bwino zofuna za msika m'mayiko osiyanasiyana, ndipo limatha kupereka zinthu zabwino kwambiri pamitengo yabwino kumisika yosiyanasiyana.Kampani yathu yakhazikitsa kale gulu loyenerera, lopanga komanso lodalirika kuti litukule makasitomala ndi mfundo zopambana zambiri.

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