Chinese Professional Inactivated Efficient Virus Preservation Solution yokhala ndi Rnase Inhibitors
Kufotokozera Kwa Zida:
◮RNase inhibitor yatsopano yopangidwa ndi mbewa yowonetsedwa mu mabakiteriya opangidwa ndi E. coli pogwiritsa ntchito ukadaulo wophatikiza ma jini.Inhibitor imalepheretsa ntchito ya RNase posaphatikizana mopikisana ndi RNase 1: 1, potero imateteza.RNAumphumphu , ndipo akhoza kulepheretsa bwinondiRNase A, B, C ntchito , koma osati RNase T1, T2, H, etc.; Ndi chosinthika cha kumanga kwa RNase Inhibitor ndi RNase , ndipo zovutazo zimatha kulekanitsidwa ndi urea ndi sulfhydryl reagents, kotero kuti RNase renatures ndi inhibitor ndi irreversibly inactivated.
Ndi njira yabwino yodalirika, mbiri yabwino komanso ntchito yabwino kwa ogula, mayankho opangidwa ndi bizinesi yathu amatumizidwa kumayiko ambiri ndi zigawo za Chinese Professional Inactivated Efficient Virus Preservation Solution ndi Rnase Inhibitors, Ndipo titha kuthandizira kuyang'ana zinthu zilizonse zomwe makasitomala amafunikira.Onetsetsani kuti mukupereka Utumiki Wabwino Kwambiri, Ubwino Wabwino Kwambiri, Kutumiza Kwachangu.
Ndi njira yabwino yodalirika, mbiri yabwino komanso ntchito yabwino kwa ogula, mayankho angapo opangidwa ndi bizinesi yathu amatumizidwa kumayiko ndi zigawo zambiri.China Virus Preservation with Rnase Inhibitors and Inactivated Virus Preservation Reagent, Kwa aliyense amene ali ndi chidwi ndi chilichonse mwazinthu zathu mukangowona mndandanda wazinthu zathu, chonde khalani omasuka kulumikizana nafe kuti mufunse mafunso.Mutha kutitumizira maimelo ndikulumikizana nafe kuti tikambirane ndipo tidzakuyankhani posachedwa momwe tingathere.Ngati ndizosavuta, mutha kupeza adilesi yathu patsamba lathu ndikubwera kubizinesi yathu kuti mudziwe zambiri za mayankho athu panokha.Takhala okonzeka nthawi zonse kupanga mgwirizano wotalikirapo komanso wosasunthika ndi makasitomala omwe angakhale nawo pazinthu zofananira.
Zofotokozera
5ml, 10ml, 40ml
PCR Hero (Wokhala Ndi Dye) Extraction kit ikuphatikiza m'badwo watsopano wapadera wa Foregene wa Taq-Foregene Taq Hero, ma dNTP okhathamiritsa, MgCl2, buffer reaction, PCR reaction enhancer, optimizer ndi stabilizer.Dongosolo la 2 × PCR Hero TM Mix (dye) lili ndi kulekerera kwakukulu kwa PCR inhibitors kuposa dongosolo wamba la PCR Mix, ndipo limatha kuthana ndi kukulitsa kwa PCR kwa ma template osiyanasiyana ovuta.Dongosolo lapadera la machitidwe a Taq Hero komanso kuchita bwino kwambiri kwa Taq Hero kumapangitsa kuti machitidwe a PCR akhale ndi magwiridwe antchito apamwamba, kutsimikizika komanso chidwi.
Zida zamagulu
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Ena stabilizers ndi enhancers |
| DNA Loading Buffer |
Mbali & ubwino
■ Kukhulupirika kwakukulu: kuwirikiza ka 6 kuposa ka enzyme wamba wa Taq;
■ Kuthamanga kwachangu kokulitsa : Kuthamanga kwa matalikidwe ndi 5-10sec/kb, yomwe ndi 6-12 nthawi ya Taq enzyme wamba;
■ Kusinthasintha kwa ma template: Imatha kukulitsa bwino ma templates osiyanasiyana ovuta a DNA ndi apamwambaZithunzi za GC;
■ Kuchulukitsitsa kwapamwamba: kuchepekera kwa kakulidwe kocheperako kuposa ma enzyme wamba a Taq;
■ Kulekerera kwa chilengedwe kumakhala kolimba: kuikidwa pa 37 ° C kwa sabata, kusunga ntchito yoposa 90%;
■ Ili ndi 5′ →3′ DNA polymerase ntchito ndi 5′→3’ exonuclease ntchito, popanda 3′ →5’ exonuclease ntchito.
■ Chidacho chimasakanikirana ndi Electrophoresis Loading Buffer, PCR itatha, chitsanzocho chikhoza kuikidwa mwachindunji kwa electrophoresis;ntchito ndi yosavuta komanso yosavuta.
Kit application
Kukulitsa kwa PCR kwa zidutswa za DNA, zilembo za DNA, kutsatizana kwa DNA, PCR kuphatikiza A mchira
Kusungirako ndi moyo wa alumali
Ikhoza kusungidwa kwa nthawi yaitali pa -20 ° C;Kugwiritsa ntchito pafupipafupi, kumatha kusungidwa pa 4 ° C kwakanthawi kochepa. Ndi njira yabwino yodalirika, mbiri yabwino komanso ntchito yabwino ya ogula, mndandanda wa mayankho opangidwa ndi bizinesi yathu umatumizidwa kumayiko ambiri ndi zigawo za Chinese Professional Inactivated Efficient Virus Preservation Solution ndi Rnase Inhibitors, Ndipo titha kuthandizira kuyang'ana zinthu zilizonse zomwe makasitomala amafuna.Onetsetsani kuti mukupereka Utumiki Wabwino Kwambiri, Ubwino Wabwino Kwambiri, Kutumiza Kwachangu.
Katswiri waku ChinaChina Virus Preservation with Rnase Inhibitors and Inactivated Virus Preservation Reagent, Kwa aliyense amene ali ndi chidwi ndi chilichonse mwazinthu zathu mukangowona mndandanda wazinthu zathu, chonde khalani omasuka kulumikizana nafe kuti mufunse mafunso.Mutha kutitumizira maimelo ndikulumikizana nafe kuti tikambirane ndipo tidzakuyankhani posachedwa momwe tingathere.Ngati ndizosavuta, mutha kupeza adilesi yathu patsamba lathu ndikubwera kubizinesi yathu kuti mudziwe zambiri za mayankho athu panokha.Takhala okonzeka nthawi zonse kupanga mgwirizano wotalikirapo komanso wosasunthika ndi makasitomala omwe angakhale nawo pazinthu zofananira.
