Tikukhulupirira kuti mgwirizano wautali udabwera chifukwa chamtundu wapamwamba kwambiri, ntchito zowonjezedwa, zokumana nazo bwino komanso kulumikizana kwanu kwa Fakitale ya Virus Magnetic Bead Method Rna-DNA Nucleic Acid 96 Sample Extraction Purification Tube Kit, Tikuwona kuti okonda, opumira komanso ophunzitsidwa bwino atha kupanga mayanjano osangalatsa komanso othandiza kwambiri ndi inu mwachangu.Onetsetsani kuti mukumva kuti ndinu omasuka kulumikizana nafe kuti mudziwe zambiri.
Timakhulupirira kuti mgwirizano wautali udabwera chifukwa chapamwamba kwambiri, mautumiki owonjezera, luso lochita bwino komanso kulumikizana kwanuChina Nucleic Acid Extraction Kit (rna-DNA) ndi 96 Nucleic Acid, Kuumirira pa kasamalidwe ka mibadwo yapamwamba kwambiri ndi chithandizo cha makasitomala odziwa bwino ntchito, tsopano tapanga lingaliro lathu kuti tipatse ogula athu kugwiritsa ntchito poyambira ndikupeza kuchuluka komanso pambuyo pazidziwitso zothandiza.Kusunga ubale waubwenzi womwe ulipo ndi ogula athu, komabe timapanga mindandanda yathu yanthawi zonse kuti tikwaniritse zofuna zatsopano ndikutsata chitukuko chaposachedwa kwambiri cha msika ku Malta.Ndife okonzeka kukumana ndi nkhawa ndikupanga kusintha kuti timvetsetse zotheka zonse zamalonda apadziko lonse lapansi.

Zofotokozera

50 Preps, poyeretsa DNA ya genomic kuchokera ku magazi athunthu pogwiritsa ntchito <1ml magazi

Buffer DC: Imatulutsa maselo ofiira m'magazi.

Buffer BL1: Perekani chitsanzo cha enzymatic digestion.

Foregene Protease Plus: Idyani zitsanzo zamagazi mwa enzyme m'malo a Buffer BL1.

Buffer BL2: Yambitsani Foregene Protease Plus ndikupereka malo otsegulira DNA.

Buffer PW: Chotsani zonyansa monga mapuloteni ndi RNA mu DNA.

Buffer WB1: Chotsani ayoni amchere otsalira mu DNA.

Buffer EB: Elute DNA pa membrane yoyeretsa.

DNA-Only Column: Imatsatsa makamaka DNA ya genomic mu lysate.

Zida zamagulu

Mbali & ubwino

-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.

-Kuthamanga Kwambiri: Foregene Protease Plus ili ndi ntchito yapamwamba kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mofulumira;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa ma genomic DNA imatha kutha mkati mwa mphindi 40.

-Kuthandizira: The centrifugation ikuchitika kutentha kwa firiji, 4 ° C centrifugation kapena ethanol mpweya wa DNA sikufunika.

-Safety: Palibe organic reagent m'zigawo zomwe zimagwiritsidwa ntchito.

-Zapamwamba: Zidutswa za DNA zomwe zatulutsidwa ndi zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira pazoyeserera zosiyanasiyana.

Kit application

Ndiwoyenera kuchotsa ndi kuyeretsa DNA ya genomic kuchokera kumagazi atsopano kapena oundana omwe ali ndi anticoagulated.

Kayendedwe kantchito

Kusungirako ndi moyo wa alumali

Chidachi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa pa 2-8 ° C.

Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa firiji kwakanthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.

Njira yothetsera Foregene Protease Plus ili ndi njira yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);idzakhala yogwira ntchito komanso yokhazikika pamene isungidwa pa 4 ° C, choncho ikulimbikitsidwa kuti isungidwe pa 4 ° C, kumbukirani kuti musaisunge pa -20 ° C. Timakhulupirira kuti mgwirizano wautali wofotokozera kwenikweni ndi chifukwa cha khalidwe lapamwamba, mautumiki owonjezera, chidziwitso chotukuka komanso kukhudzana kwaumwini kwa Fakitale Malonda a Virus Magnetic Bead Njira Rna-DNA Nucleic Acid , Purification Kuphwanya Ntchito Yowonongeka, Kuyeretsa Tube Yogwira Ntchito Yogwira Ntchito Yomangamanga, Kuyeretsa Tube ndi Kuphwanya Ntchito Yowonongeka mphamvu imatha kupanga mayanjano abwino kwambiri komanso othandiza mabizinesi ndi inu mwachangu.Onetsetsani kuti mukumva kuti ndinu omasuka kulumikizana nafe kuti mudziwe zambiri.
Malo ogulitsa mafakitale kwaChina Nucleic Acid Extraction Kit (rna-DNA) ndi 96 Nucleic Acid, Kuumirira pa kasamalidwe ka mibadwo yapamwamba kwambiri ndi chithandizo cha makasitomala odziwa bwino ntchito, tsopano tapanga lingaliro lathu kuti tipatse ogula athu kugwiritsa ntchito poyambira ndikupeza kuchuluka komanso pambuyo pazidziwitso zothandiza.Kusunga ubale waubwenzi womwe ulipo ndi ogula athu, komabe timapanga mindandanda yathu yanthawi zonse kuti tikwaniritse zofuna zatsopano ndikutsata chitukuko chaposachedwa kwambiri cha msika ku Malta.Ndife okonzeka kukumana ndi nkhawa ndikupanga kusintha kuti timvetsetse zotheka zonse zamalonda apadziko lonse lapansi.