Fakitale imaperekedwa 0.2ml Flat Cap Transparent PCR Tube
Kufotokozera Kwa Zida:
◮RNase inhibitor yatsopano yopangidwa ndi mbewa yowonetsedwa mu mabakiteriya opangidwa ndi E. coli pogwiritsa ntchito ukadaulo wophatikiza ma jini.Inhibitor imalepheretsa ntchito ya RNase posaphatikizana mopikisana ndi RNase 1: 1, potero imateteza.RNAumphumphu , ndipo akhoza kulepheretsa bwinondiRNase A, B, C ntchito , koma osati RNase T1, T2, H, etc.; Ndi chosinthika cha kumanga kwa RNase Inhibitor ndi RNase , ndipo zovutazo zimatha kulekanitsidwa ndi urea ndi sulfhydryl reagents, kotero kuti RNase renatures ndi inhibitor ndi irreversibly inactivated.
Timadalira kuganiza mwanzeru, kusinthika kosalekeza m'magawo onse, kupita patsogolo kwaukadaulo komanso kwa ogwira ntchito athu omwe amatenga nawo gawo pachipambano chathu ku Factory yomwe yaperekedwa 0.2ml Flat Cap Transparent PCR Tube, Tikulandilani ndi manja awiri kutenga nawo gawo kutengera mapindu owonjezera omwe akubwera posachedwa.
Timadalira kuganiza mwanzeru, kusinthika kosalekeza m'magawo onse, kupita patsogolo kwaukadaulo komanso kwa antchito athu omwe amatenga nawo gawo pakuchita bwino kwathu.China PCR Tube ndi 0.2ml PCR Tube, Mayankho athu atumizidwa makamaka kumwera chakum'mawa kwa Asia Euro-America, ndikugulitsa kudziko lathu lonse.Ndipo kutengera zabwino kwambiri, mtengo wololera, ntchito yabwino kwambiri, tili ndi mayankho abwino kuchokera kwamakasitomala akunja.Mwalandiridwa kuti mubwere nafe kuti mupeze zambiri komanso zopindulitsa.Tikulandira makasitomala, mabungwe ochita bizinesi ndi abwenzi ochokera kumadera onse adziko lapansi kuti atilumikizane ndikupempha mgwirizano kuti tipindule.
Zofotokozera
5ml, 10ml, 40ml
PCR Hero (Wokhala Ndi Dye) Extraction kit ikuphatikiza m'badwo watsopano wapadera wa Foregene wa Taq-Foregene Taq Hero, ma dNTP okhathamiritsa, MgCl2, buffer reaction, PCR reaction enhancer, optimizer ndi stabilizer.Dongosolo la 2 × PCR Hero TM Mix (dye) lili ndi kulekerera kwakukulu kwa PCR inhibitors kuposa dongosolo wamba la PCR Mix, ndipo limatha kuthana ndi kukulitsa kwa PCR kwa ma template osiyanasiyana ovuta.Dongosolo lapadera la machitidwe a Taq Hero komanso kuchita bwino kwambiri kwa Taq Hero kumapangitsa kuti machitidwe a PCR akhale ndi magwiridwe antchito apamwamba, kutsimikizika komanso chidwi.
Zida zamagulu
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Ena stabilizers ndi enhancers |
| DNA Loading Buffer |
Mbali & ubwino
■ Kukhulupirika kwakukulu: kuwirikiza ka 6 kuposa ka enzyme wamba wa Taq;
■ Kuthamanga kwachangu kokulitsa : Kuthamanga kwa matalikidwe ndi 5-10sec/kb, yomwe ndi 6-12 nthawi ya Taq enzyme wamba;
■ Kusinthasintha kwa ma template: Imatha kukulitsa bwino ma templates osiyanasiyana ovuta a DNA ndi apamwambaZithunzi za GC;
■ Kuchulukitsitsa kwapamwamba: kuchepekera kwa kakulidwe kocheperako kuposa ma enzyme wamba a Taq;
■ Kulekerera kwa chilengedwe kumakhala kolimba: kuikidwa pa 37 ° C kwa sabata, kusunga ntchito yoposa 90%;
■ Ili ndi 5′ →3′ DNA polymerase ntchito ndi 5′→3’ exonuclease ntchito, popanda 3′ →5’ exonuclease ntchito.
■ Chidacho chimasakanikirana ndi Electrophoresis Loading Buffer, PCR itatha, chitsanzocho chikhoza kuikidwa mwachindunji kwa electrophoresis;ntchito ndi yosavuta komanso yosavuta.
Kit application
Kukulitsa kwa PCR kwa zidutswa za DNA, zilembo za DNA, kutsatizana kwa DNA, PCR kuphatikiza A mchira
Kusungirako ndi moyo wa alumali
Ikhoza kusungidwa kwa nthawi yaitali pa -20 ° C;kuti tigwiritse ntchito pafupipafupi, imatha kusungidwa pa 4 ° C kwakanthawi kochepa. Timadalira kuganiza mwanzeru, kusinthika kosalekeza m'magulu onse, kupita patsogolo kwaukadaulo komanso kwa ogwira ntchito athu omwe amatenga nawo gawo pakuchita bwino kwathu kwa Factory yoperekedwa ndi 0.2ml Flat Cap Transparent PCR Tube, Tikulandila ndi manja awiri kutengapo gawo kwanu kumadalira mapindu owonjezera omwe akubwera.
Fakitale yaperekedwaChina PCR Tube ndi 0.2ml PCR Tube, Mayankho athu atumizidwa makamaka kumwera chakum'mawa kwa Asia Euro-America, ndikugulitsa kudziko lathu lonse.Ndipo kutengera zabwino kwambiri, mtengo wololera, ntchito yabwino kwambiri, tili ndi mayankho abwino kuchokera kwamakasitomala akunja.Mwalandiridwa kuti mubwere nafe kuti mupeze zambiri komanso zopindulitsa.Tikulandira makasitomala, mabungwe ochita bizinesi ndi abwenzi ochokera kumadera onse adziko lapansi kuti atilumikizane ndikupempha mgwirizano kuti tipindule.
