China Ubwino Wabwino China Bioer Biospin DNA / Rna Extraction Kit 50t Reagent Manufacturer ndi Supplier |Foregene

Ubwino Wabwino wa China Bioer Biospin DNA / Rna Extraction Kit 50t Reagent

Ubwino Wabwino waku China Bioer Biospin DNA / Rna Extraction Kit 50t Reagent Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito ukadaulo wa Real-time RT PCR (rRT-PCR) pozindikira bwino za SARS-CoV-2 (ORF1ab jini ndi N jini) ma nucleic acid mu zitsanzo za nasopharyngeal kapena oropharyngeal swab, pogwiritsa ntchito multiplex PCR fulorosenti probe method. kuzindikira mwachangu kwambiri.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Nthawi zambiri kumawonjezera pulogalamu yoyang'anira makonzedwe chifukwa chaukadaulo wa "chikhulupiriro chabwino, chatsopano chomwe chimakhala ndi gawo lazinthu zokhudzana ndi makasitomala,Zida Zotulutsa50t Reagent, Chiyambireni gawo lopanga zinthu lomwe lidakhazikitsidwa, tsopano tadzipereka pakupititsa patsogolo zinthu zatsopano.Pamodzi ndi mayendedwe azachuma komanso azachuma, tipitiliza kupititsa patsogolo mzimu wa "zapamwamba kwambiri, zogwira mtima, zaluso, kukhulupirika", ndikukhala ndi mfundo za "ngongole poyambirira, kasitomala woyamba, wabwino kwambiri".Tipanga tsogolo labwino kwambiri pakutulutsa tsitsi ndi anzathu.
Kupititsa patsogolo pulogalamu yoyang'anira pafupipafupi paulamuliro wa "wowona mtima, chikhulupiriro chabwino kwambiri komanso chapamwamba ndiye maziko a chitukuko cha bizinesi", timatengera zomwe zili muzinthu zokhudzana ndi dziko lonse lapansi, ndikupanga zatsopano kuti zikwaniritse zomwe makasitomala amafuna.China Dbiozol Genomic DNA, Zida Zotulutsa, Chikhulupiriro chathu ndi kukhala owona mtima choyamba, kotero ife timangopereka zinthu zapamwamba kwa makasitomala athu.Kwenikweni ndikuyembekeza kuti titha kukhala mabizinesi.Timakhulupirira kuti tikhoza kukhazikitsa ubale wautali wamalonda wina ndi mzake.Mutha kulumikizana nafe kwaulere kuti mumve zambiri komanso mndandanda wamitengo yathu ndi mayankho!

Zofotokozera

48 Rxns, 96 Rxns

Ayi.	Chigawo	Ndalama				Zigawo zazikulu
Ayi.	Chigawo	48 nsi		96 nsi		Zigawo zazikulu
1	Nucleic acid kumasula wothandizira	1.4mL / chubu	2 machubu	5.3 mL / botolo	1 botolo	Wokwera pamwamba
2	Chitetezo cha RNA	27 μL / chubu	1 chubu	53 μL / chubu	1 chubu	RNase inhibitor
3	SARS-CoV-2 reaction solution	800 μL / chubu	1 chubu	1600 μL / chubu	1 chubu	Poyamba, probe, reaction buffer, dNTP
4	SARS-CoV-2 enzyme osakaniza	80 μL / chubu	1 chubu	160 μL / chubu	1 chubu	Kutentha koyambira kwa Taq enzyme, enzyme ya M-MLV
5	Kuwongolera kwabwino kwa SARS-CoV-2	100 μL / chubu	1 chubu	100 μL / chubu	1 chubu	Recombinant plasmid yokhala ndi chidutswa chandamale, RNA
6	Kuwongolera koyipa kwa SARS-CoV-2	1200 μL / chubu	1 chubu	1200 μL / chubu	1 chubu	TE buffer

Mbali & ubwino

Mwamsanga ndi zosavuta.
Pewani kutaya kwa nucleic acid.
Malire odziwikiratu otsika komanso kukhudzika kwakukulu.
Kufunika kwa zida zochepa komanso kugwiritsa ntchito kosavuta.

Kuzindikira kumatha kufika pamlingo wotsika kwambiri ngati makope 500/mL).

GCE/mL	Mtengo wapatali wa magawo Ct
GCE/mL	N gene	Mtengo wa ORF1ab
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N / A	N / A
2.00E-01	N / A	N / A
NC	N / A	N / A

Linear amplification curve

amplification curve-RT-qPCR

Momwe mungagwiritsire ntchito

1. Chida chothandizira

covid-19 nucleic acid kuzindikira zida1

2. Njira yodziwira

covid-19 nucleic acid kuzindikira zida2

Kukonzekera kwachitsanzo: Naopharyngeal kapena oropharyngeal swab chitsanzo (ntchito imodzi yokha) (Dikirani kwa mphindi 10)

PCR amplificaton(<=55min)

Kayendedwe kantchito:

covid-19 nucleic acid kuzindikira zida4

Kusungirako

osindikizidwa kuchokera kuwala ndi kusungidwa pa -20 ± 5 ℃;

yosungirako
yosungirako2

Alumali moyo:1 chaka

Nthawi zambiri kumawonjezera pulogalamu yoyang'anira makonzedwe chifukwa chaukadaulo wa "chikhulupiriro chabwino, chatsopano chomwe chimakhala ndi gawo lazinthu zokhudzana ndi makasitomala,Zida Zotulutsa50t Reagent, Chiyambireni gawo lopanga zinthu lomwe lidakhazikitsidwa, tsopano tadzipereka pakupititsa patsogolo zinthu zatsopano.Pamodzi ndi mayendedwe azachuma komanso azachuma, tipitiliza kupititsa patsogolo mzimu wa "zapamwamba kwambiri, zogwira mtima, zaluso, kukhulupirika", ndikukhala ndi mfundo za "ngongole poyambirira, kasitomala woyamba, wabwino kwambiri".Tipanga tsogolo labwino kwambiri pakutulutsa tsitsi ndi anzathu.
Ubwino WabwinoChina Dbiozol Genomic DNA, Zida Zam'zigawo, Chikhulupiriro chathu ndikukhala oona mtima poyamba, kotero timangopereka zinthu zapamwamba kwa makasitomala athu.Kwenikweni ndikuyembekeza kuti titha kukhala mabizinesi.Timakhulupirira kuti tikhoza kukhazikitsa ubale wautali wamalonda wina ndi mzake.Mutha kulumikizana nafe kwaulere kuti mumve zambiri komanso mndandanda wamitengo yathu ndi mayankho!

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