China High tanthauzo China Nucleic Acid Kuyeretsedwa m'zigawo Yc-701 Cov Nucleic Acid Mlengi ndi Supplier |Foregene

Kutanthauzira kwakukulu China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwachangu ma DNA amtundu wa nyama pazochitika za PCR, kotero ndiyoyenera kuyesa kwakukulu kwa majini.

Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65.°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2×PCR Easy^TMMix (UNG) amagwiritsa ntchito dUTP m'malo mwa dTTP pamaziko a 2×PCR Easy^TMSakanizani, ndikuwonjezera UNG enzyme (Uracil-N-glycosylase) yomwe ingawononge template yomwe ili ndi dUTP nthawi yomweyo.PCR isanachitike, puloteni ya UNG imagwiritsidwa ntchito kusokoneza mankhwala a PCR okhala ndi uracil.Enzyme ya UNG sidzakhala ndi zotsatira pa template yomwe ilibe uracil, potero imatsimikizira kutsimikizika ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuipitsidwa kwa zinthu za PCR pakuyesa kwakukulu kwa majini.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Timamamatira ku mfundo ya "ubwino woyamba, ntchito choyamba, kuwongolera kosalekeza ndi luso lokumana ndi makasitomala" kwa oyang'anira ndi "chilema, zodandaula ziro" monga cholinga chapamwamba.Kuti ntchito yathu ikhale yabwino, timapereka zinthuzo ndi zabwino pamtengo wokwanira wa Kutanthauzira Kwapamwamba China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid, kutsindika kwapadera pakupanga zinthu kuti zisawonongeke panthawi yamayendedwe, chidwi chatsatanetsatane pamayankho ndi njira za ogula athu olemekezeka.
Timamamatira ku mfundo ya "ubwino woyamba, ntchito choyamba, kuwongolera kosalekeza ndi luso lokumana ndi makasitomala" kwa oyang'anira ndi "chilema, zodandaula ziro" monga cholinga chapamwamba.Kuti tikwaniritse ntchito yathu, timapereka zinthuzo ndi khalidwe labwino pamtengo wokwaniraChina Nucleic Acid M'zigawo System, Nucleic Acid M'zigawo Chida, Tikukulitsa gawo lathu la msika wapadziko lonse lapansi kutengera zinthu zabwino, ntchito zabwino kwambiri, mtengo wokwanira komanso kutumiza munthawi yake.Chonde titumizireni nthawi iliyonse kuti mudziwe zambiri.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	Bafa AL
	Foregene Protease
	6 × DNA Loading Buffer
Gawo II	2 × PCR Yosavuta^TMMix(UNG)

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo

■ Kufuna kwachitsanzo ndikochepa, monga momwe minofu yanyama ya 5mg ingayesedwe.

■ Palibe chithandizo chapadera monga kugaya ndi kuphwanya chofunika, ndipo opaleshoniyo ndi yosavuta.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

■ Anti-kuipitsa PCR dongosolo 2 × PCR Easy^TMMix (UNG) imathetsa bwino kuipitsidwa kwa zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Zosintha za Kit

Ntchito: zosiyanasiyana nyama zimakhala.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

wk flw-nyama minofu yolunjika PCR

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer AL ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

Reagent Foregene Protease ili ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

Reagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

Reagent 2 × PCR Yosavuta^TMSakanizani (UNG), ngati imagwiritsidwa ntchito pafupipafupi, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (kugwiritsa ntchito mkati mwa masiku 10) .Timamatira ku mfundo ya "khalidwe loyamba, ntchito yoyamba, kuwongolera kosalekeza ndi luso lokumana ndi makasitomala" kwa oyang'anira ndi "chilema, ziro zodandaula" monga cholinga chaubwino.Kuti ntchito yathu ikhale yabwino, timapereka zinthuzo ndi zabwino pamtengo wokwanira wa Kutanthauzira Kwapamwamba China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid, kutsindika kwapadera pakupanga zinthu kuti zisawonongeke panthawi yamayendedwe, chidwi chatsatanetsatane pamayankho ndi njira za ogula athu olemekezeka.
Kutanthauzira kwakukuluChina Nucleic Acid M'zigawo System, Nucleic Acid M'zigawo Chida, Tikukulitsa gawo lathu la msika wapadziko lonse lapansi kutengera zinthu zabwino, ntchito zabwino kwambiri, mtengo wokwanira komanso kutumiza munthawi yake.Chonde titumizireni nthawi iliyonse kuti mudziwe zambiri.

Zam'mbuyo: Gulu Lapamwamba China Virus Nucleic Acid Extraction Kit, Nucleic Acid Test Box, Nucleic Acid

Ena: Mtengo wokwanira wa China Real Time Fluorescence Virus Nucleic Acid PCR Test

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