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Wopanga Wotsogola wa China Magnetic Beads Njira DNA Rna Nucleic Acid Extraction Reagent Kits for Real Time PCR Kuzindikira

Wopanga Wotsogola wa China Magnetic Beads Njira DNA Rna Nucleic Acid Extraction Reagent Kits for Real Time Kuzindikira kwa PCR Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito ukadaulo wa Real-time RT PCR (rRT-PCR) pozindikira bwino za SARS-CoV-2 (ORF1ab jini ndi N jini) ma nucleic acid mu zitsanzo za nasopharyngeal kapena oropharyngeal swab, pogwiritsa ntchito multiplex PCR fulorosenti probe method. kuzindikira mwachangu kwambiri.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Tikufuna kuwona kuwonongeka kwabwino pakupanga ndikupereka chithandizo chothandiza kwambiri kwa ogula akunyumba ndi akunja ndi mtima wonse kwa Wopanga Wotsogola wa China Magnetic Beads Method DNA Rna Nucleic Acid Extraction Reagent Kits for Real Time PCR Kuzindikira, Timaonetsetsanso kuti assortment yanu ipangidwa mwaluso ndikugwiritsa ntchito kudalirika.Onetsetsani kuti muli ndi ufulu wolumikizana nafe kuti mudziwe zambiri komanso zowona.
Tikufuna kuwona kuwonongeka kwabwino mkati mwazopanga ndikupereka chithandizo chothandiza kwambiri kwa ogula am'nyumba ndi akunja ndi mtima wonseAutomatic Nucleic Acid Kuyeretsa, China Universal Rna DNA Reagents, Cholinga chathu ndi kuthandiza makasitomala kuzindikira zolinga zawo.Tikuchita khama kwambiri kuti tikwaniritse izi ndikukulandirani ndi mtima wonse kuti mugwirizane nafe.Mwachidule, mukamasankha ife, mumasankha moyo wangwiro.Takulandilani kukaona fakitale yathu ndikulandila oda yanu!Kuti mudziwe zambiri, musazengereze kulumikizana nafe.

Zofotokozera

48 Rxns, 96 Rxns

Ayi.	Chigawo	Ndalama				Zigawo zazikulu
Ayi.	Chigawo	48 nsi		96 nsi		Zigawo zazikulu
1	Nucleic acid kumasula wothandizira	1.4mL / chubu	2 machubu	5.3 mL / botolo	1 botolo	Wokwera pamwamba
2	Chitetezo cha RNA	27 μL / chubu	1 chubu	53 μL / chubu	1 chubu	RNase inhibitor
3	SARS-CoV-2 reaction solution	800 μL / chubu	1 chubu	1600 μL / chubu	1 chubu	Poyamba, probe, reaction buffer, dNTP
4	SARS-CoV-2 enzyme osakaniza	80 μL / chubu	1 chubu	160 μL / chubu	1 chubu	Kutentha koyambira kwa Taq enzyme, enzyme ya M-MLV
5	Kuwongolera kwabwino kwa SARS-CoV-2	100 μL / chubu	1 chubu	100 μL / chubu	1 chubu	Recombinant plasmid yokhala ndi chidutswa chandamale, RNA
6	Kuwongolera koyipa kwa SARS-CoV-2	1200 μL / chubu	1 chubu	1200 μL / chubu	1 chubu	TE buffer

Mbali & ubwino

Mwamsanga ndi zosavuta.
Pewani kutaya kwa nucleic acid.
Malire odziwikiratu otsika komanso kukhudzika kwakukulu.
Kufunika kwa zida zochepa komanso kugwiritsa ntchito kosavuta.

Kuzindikira kumatha kufika pamlingo wotsika kwambiri ngati makope 500/mL).

GCE/mL	Mtengo wapatali wa magawo Ct
GCE/mL	N gene	Mtengo wa ORF1ab
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N / A	N / A
2.00E-01	N / A	N / A
NC	N / A	N / A

Linear amplification curve

amplification curve-RT-qPCR

Momwe mungagwiritsire ntchito

1. Chida chothandizira

covid-19 nucleic acid kuzindikira zida1

2. Njira yodziwira

covid-19 nucleic acid kuzindikira zida2

Kukonzekera kwachitsanzo: Naopharyngeal kapena oropharyngeal swab chitsanzo (ntchito imodzi yokha) (Dikirani kwa mphindi 10)

PCR amplificaton(<=55min)

Kayendedwe kantchito:

covid-19 nucleic acid kuzindikira zida4

Kusungirako

osindikizidwa kuchokera kuwala ndi kusungidwa pa -20 ± 5 ℃;

yosungirako
yosungirako2

Alumali moyo:1 chaka

Tikufuna kuwona kuwonongeka kwabwino pakupanga ndikupereka chithandizo chothandiza kwambiri kwa ogula akunyumba ndi akunja ndi mtima wonse kwa Wopanga Wotsogola wa China Magnetic Beads Method DNA Rna Nucleic Acid Extraction Reagent Kits for Real Time PCR Kuzindikira, Timaonetsetsanso kuti assortment yanu ipangidwa mwaluso ndikugwiritsa ntchito kudalirika.Onetsetsani kuti muli ndi ufulu wolumikizana nafe kuti mudziwe zambiri komanso zowona.
Mtsogoleri Wopanga kwaChina Universal Rna DNA Reagents, Automatic Nucleic Acid Kuyeretsa, Cholinga chathu ndi kuthandiza makasitomala kuzindikira zolinga zawo.Tikuchita khama kwambiri kuti tikwaniritse izi ndikukulandirani ndi mtima wonse kuti mugwirizane nafe.Mwachidule, mukamasankha ife, mumasankha moyo wangwiro.Takulandilani kukaona fakitale yathu ndikulandila oda yanu!Kuti mudziwe zambiri, musazengereze kulumikizana nafe.

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