Zomwe zili ndi malingaliro abwino komanso opita patsogolo ku chidwi chamakasitomala, bizinesi yathu nthawi zonse imasintha zinthu zathu kuti zikwaniritse zilakolako za makasitomala komanso imayang'ana kwambiri chitetezo, kudalirika, zofuna zachilengedwe, komanso luso la wopanga China Magnetic Beads Nucleic Acid PCR Extraction Kit ndi CE ya Poct PCR Machine, Mowona mtima tikukula pamodzi ndi chiyembekezo chathu chonse.
Zomwe zili ndi malingaliro abwino komanso opita patsogolo ku chidwi cha kasitomala, bizinesi yathu nthawi zonse imasintha zinthu zathu kuti zikwaniritse zokhumba za makasitomala ndikuwunikanso chitetezo, kudalirika, zofuna zachilengedwe, komanso luso laukadauloChina DNA Extraction Kit, Nucleic Acid Extraction Kit, Timatsatira kasitomala woyamba, wapamwamba kwambiri 1, kuwongolera mosalekeza, kupindulitsana komanso mfundo zopambana.Tikamathandizana ndi kasitomala, timatumiza ogula ndi ntchito zapamwamba kwambiri.Takhazikitsa ubale wabwino wamabizinesi pogwiritsa ntchito wogula waku Zimbabwe mkati mwa bizinesi, takhazikitsa dzina lathu komanso mbiri yathu.Nthawi yomweyo, landirani ndi mtima wonse ziyembekezo zatsopano ndi zakale ku kampani yathu kupita kukakambirana mabizinesi ang'onoang'ono.

Kufotokozera

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer yomwe imatha kumasula mwachangu RNA kuchokera ku ma cell otukuka pamachitidwe a RT-qPCR, potero amachotsa njira yoyeretsera RNA yomwe imatenga nthawi komanso yovuta.The RNA template ikhoza kupezeka mu mphindi 7 zokha.5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR reagents zoperekedwa ndi zida zitha kupeza mwachangu komanso moyenera zotsatira za PCR zenizeni zenizeni.

5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR ali ndi kulekerera kwamphamvu kwa inhibitor, ndipo ma lysate a zitsanzo angagwiritsidwe ntchito ngati template ya RT-qPCR mwachindunji.Chidachi chili ndi RNA yapamwamba kwambiri ya Foregene reverse transcriptase, ndi Hot D-Taq DNA polymerase, dNTPs, MgCl.₂, zotchingira zochita, PCR optimizer ndi stabilizer.

Zofotokozera

200×20μl Rxns, 1000×20μl Rxns

Zida zamagulu

Mbali & ubwino

■ Zosavuta komanso zothandiza : ndiukadaulo wa Cell Direct RT, zitsanzo za RNA zitha kupezeka m'mphindi 7 zokha.

■ Zofuna zachitsanzo ndizochepa, mpaka ma cell 10 akhoza kuyesedwa.

■ Kutulutsa kwakukulu: kumatha kuzindikira mwachangu RNA m'maselo opangidwa mu 384, 96, 24, 12, 6-chitsime mbale.

■ DNA chofufutira akhoza kuchotsa mwamsanga matupi athu omasulidwa, kwambiri kuchepetsa zotsatira zoyeserera.

■ Makina okhathamiritsa a RT ndi qPCR amapangitsa kuti masitepe awiri a RT-PCR alembedwe bwino kwambiri komanso PCR yachindunji, komanso yosagwirizana ndi RT-qPCR reaction inhibitors.

Kit application

Kuchuluka kwa ntchito: maselo otukuka.

- RNA yotulutsidwa ndi zitsanzo za lysis: imagwira ntchito pa template ya RT-qPCR ya zida izi.

- Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kusanthula kwa jini, kutsimikizira kwa siRNA-mediated gene silence effect, kuyang'anira mankhwala, etc.

Chithunzi

Kusungirako ndi moyo wa alumali

Gawo I la zida izi ziyenera kusungidwa pa 4 ℃;Gawo II liyenera kusungidwa pa -20 ℃.

Foregene Protease Plus II iyenera kusungidwa pa 4 ℃, osazizira pa -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR iyenera kusungidwa pa -20 ℃ mumdima;ngati ntchito kawirikawiri, zikhoza kusungidwa pa 4 ℃ kwa yosungirako yochepa (ntchito mkati masiku 10) .Omwe ali ndi maganizo abwino ndi pang'onopang'ono kwa chidwi kasitomala, ntchito yathu mosasintha kusintha katundu wathu kwambiri kukhutiritsa zilakolako za makasitomala ndi zina zimayang'ana pa chitetezo, kudalirika, zofuna zachilengedwe, ndi luso la opanga makina opanga maginito makina CR, China ndi makina opanga makina CR, China ndi makina opangira maginito. Ndikukhulupirira kuti tikukula limodzi ndi ziyembekezo zathu padziko lonse lapansi.
Wopanga kwaChina DNA Extraction Kit, Nucleic Acid Extraction Kit, Timatsatira kasitomala woyamba, wapamwamba kwambiri 1, kuwongolera mosalekeza, kupindulitsana komanso mfundo zopambana.Tikamathandizana ndi kasitomala, timatumiza ogula ndi ntchito zapamwamba kwambiri.Takhazikitsa ubale wabwino wamabizinesi pogwiritsa ntchito wogula waku Zimbabwe mkati mwa bizinesi, takhazikitsa dzina lathu komanso mbiri yathu.Nthawi yomweyo, landirani ndi mtima wonse ziyembekezo zatsopano ndi zakale ku kampani yathu kupita kukakambirana mabizinesi ang'onoang'ono.