Ziribe kanthu wogula watsopano kapena kasitomala wakale, Timakhulupirira kuyankhulana kwautali komanso ubale wodalirika wa OEM Supply High Quality Magnetic Beads Virus DNA Nucleic Acid Extraction kapena Purification Kit, Timalandira mwachikondi makasitomala, mabizinesi ndi mabwenzi ochokera padziko lonse lapansi kuti alumikizane nafe ndikupempha mgwirizano kuti mupindule nawo.
Ziribe kanthu wogula watsopano kapena kasitomala wakale, Timakhulupirira mukulankhula kwautali komanso ubale wodalirikaChina PCR ndi Nucleic Acid Test Kit, Kaya mukusankha zomwe zilipo pakalipano kapena kufunafuna thandizo la uinjiniya kuti mugwiritse ntchito, mutha kulankhula ndi malo athu othandizira makasitomala za zomwe mukufuna kupeza.Takhala tikuyembekezera kugwirizana ndi anzathu ochokera padziko lonse lapansi.
Mabuku a Malangizo:

Ziribe kanthu wogula watsopano kapena kasitomala wakale, Timakhulupirira kuyankhulana kwautali komanso ubale wodalirika wa OEM Supply High Quality Magnetic Beads Virus DNA Nucleic Acid Extraction kapena Purification Kit, Timalandira mwachikondi makasitomala, mabizinesi ndi mabwenzi ochokera padziko lonse lapansi kuti alumikizane nafe ndikupempha mgwirizano kuti mupindule nawo.
OEM SupplyChina PCR ndi Nucleic Acid Test Kit, Kaya mukusankha zomwe zilipo pakalipano kapena kufunafuna thandizo la uinjiniya kuti mugwiritse ntchito, mutha kulankhula ndi malo athu othandizira makasitomala za zomwe mukufuna kupeza.Takhala tikuyembekezera kugwirizana ndi anzathu ochokera padziko lonse lapansi.

Vuto Kusanthula Buku

Zotsatirazi ndikuwunika mavuto omwe angakumane nawo pochotsa ma virus a DNA / RNA, ndikuyembekeza kukhala zothandiza pazoyeserera zanu.Kuphatikiza apo, pazovuta zina zoyesera kapena zaukadaulo kupatula malangizo ogwiritsira ntchito ndikuwunika zovuta, tapereka chithandizo chaukadaulo kukuthandizani.Ngati muli ndi zosowa, chonde titumizireni: 028-83360257 kapena Imelo:

Tech@foregene.com.

Palibe kutulutsa kwa nucleic acid kapena kutsika kwa nucleic acid

Nthawi zambiri pamakhala zinthu zambiri zomwe zimakhudza kuchira bwino, monga: zitsanzo za nucleic acid, njira yogwirira ntchito, voliyumu ya elution, ndi zina zambiri.

Kusanthula kwazomwe zimayambitsa:

1. Kusambira kwa ayezi kapena kutentha kochepa (4 ° C) centrifugation kunkachitika panthawiyi.

Malangizo: Gwiritsani ntchito kutentha kwapakati (15-25 ° C) panthawi yonseyi, musamasambitse madzi oundana komanso kutentha kwa centrifugation.

2. Chitsanzocho chinasungidwa molakwika kapena chitsanzocho chinasungidwa kwa nthawi yayitali.

Malangizo: Sungani zitsanzo pa -80 ° C ndipo pewani kuzizira mobwerezabwereza ndi kusungunuka;yesetsani kugwiritsa ntchito zitsanzo zomwe zasonkhanitsidwa mwatsopano pochotsa nucleic acid.

3. Kusakwanira kwachitsanzo lysis.

Malangizo: Chonde onetsetsani kuti sampuli ndi njira yogwirira ntchito ya lysis yasakanizidwa bwino ndikuyikidwa pa kutentha kwapakati (15-25 ° C) kwa mphindi 10.

4. Kuwonjeza molakwika kwa eluent.

Yesani: Onetsetsani kuti RNase-Free ddH2O yawonjezedwa pang'onopang'ono pakati pa nembanemba yoyeretsa, ndipo musayigwetse pa mphete yoyeretsera.

5. Voliyumu yolondola ya ethanol yokwanira sinawonjezedwe ku Buffer RW2.

Malingaliro: Chonde tsatirani malangizo, onjezani voliyumu yolondola ya ethanol ku Buffer RW2 ndikusakaniza bwino musanagwiritse ntchito zida.

6. Voliyumu yachitsanzo yosayenera.

Lingaliro: 200µl ya zitsanzo imakonzedwa pa 500µl iliyonse ya Buffer DRL.Kuchulukitsidwa kwachitsanzo kumapangitsa kuti pakhale zokolola zochepa za nucleic acid.

7. Voliyumu yofotokozera molakwika kapena kusakwanira.

Malangizo: Voliyumu yodziwika bwino ya gawo loyeretsa ndi 30-50μl;ngati elution zotsatira si zogwira mtima, Ndi bwino kuwonjezera nthawi firiji pambuyo kuwonjezera preheated RNase-Free ddH2O, monga 5-10min.

8. Mowa amakhalabe pamzati mutatsuka ndi Buffer RW2.

Malingaliro: Ngati Mowa utsalira pambuyo centrifugation ndi Buffer RW2 kwa mphindi 2, ndime akhoza kuikidwa pa firiji kwa mphindi 5 pambuyo centrifugation kuchotsa kwathunthu Mowa yotsalira.

Nucleic acid yoyeretsedwa imawonongeka

Ubwino wa nucleic acid woyeretsedwa umagwirizana ndi kusungidwa kwa chitsanzo, kuipitsidwa kwa RNase, ntchito ndi zina.Kusanthula kwazomwe zimayambitsa:

1. Zitsanzo zosonkhanitsidwa sizinasungidwe mu nthawi.

Lingaliro: Ngati chitsanzocho sichigwiritsidwa ntchito pakapita nthawi pambuyo pa kusonkhanitsa, chonde sungani pa -80 ° C pa kutentha kochepa nthawi yomweyo.Pochotsa RNA, yesani kugwiritsa ntchito zitsanzo zomwe zasonkhanitsidwa mwatsopano.

2. Sonkhanitsani zitsanzo ndikuundana ndikusungunuka mobwerezabwereza.

Yesani: Pewani kuzizira ndi kusungunuka (osapitirira kamodzi) panthawi yosonkhanitsa ndi kusunga zitsanzo, apo ayi zokolola za nucleic acid zidzachepa.

3. RNase imayambitsidwa mu chipinda cha opaleshoni kapena magolovesi otayika, masks, ndi zina.

Malangizo: Kuyesera kwa RNA m'zigawo kumachitidwa bwino m'chipinda chapadera cha RNA, ndipo tebulo la labotale liyenera kutsukidwa musanayese.

Valani magolovesi ndi masks otayika panthawi yoyesera kuti mupewe kuwonongeka kwa RNA komwe kumachitika chifukwa cha kukhazikitsidwa kwa RNase kwambiri.

4. The reagent anali zoipitsidwa ndi RNase pa ntchito.

Malangizo: Bwezerani ndi Viral DNA/RNA Isolation Kit yatsopano pazoyeserera zofananira.

5. Machubu a centrifuge ndi malangizo a pipette omwe amagwiritsidwa ntchito posokoneza RNA ali ndi kachilombo ka RNase.

Yesani: Onetsetsani kuti machubu a centrifuge, nsonga za pipette, mapaipi, ndi zina zomwe zimagwiritsidwa ntchito pochotsa RNA zonse ndi zopanda RNase.

Kuyeretsedwa kwa nucleic acid kumakhudza zoyesa zapansi

DNA ndi RNA zoyeretsedwa ndi gawo loyeretsa, ngati mchere wa ion ndi mapuloteni ochuluka kwambiri, zidzakhudza kuyesa kutsika kwapansi, monga: kukulitsa kwa PCR, reverse transcript, etc.

1. Ma DNA ochotsedwa ndi RNA ali ndi ayoni amchere otsalira.

Lingaliro: Onetsetsani kuti voliyumu yolondola ya ethanol ionjezedwa ku Buffer RW2, ndikutsuka ndime yoyeretsera kawiri pa liwiro la centrifugation lomwe lafotokozedwa mu malangizo ogwiritsira ntchito;Pangani centrifugation kuti muchepetse kuipitsidwa kwa ion ion.

2. DNA ndi RNA zochotsedwa zili ndi zotsalira za ethanol.

Malingaliro: Pambuyo potsimikizira kutsuka ndi Buffer RW2, chitani chopanda kanthu chubu centrifugation pa centrifugation liwiro mu malangizo ntchito;ngati pali zotsalira za ethanol, mutha centrifuge chubu chopanda kanthu ndikuchiyika pamalo otentha kwa mphindi 5 kuti muchotse zotsalira za ethanol kwambiri.

Mabuku a Malangizo:

Viral DNA&RNA Isolation Kit Instruction Manual