China OEM/ODM Factory China Magnetic Beads Method Nucleic Acid Rna Extraction Reagent Kits for Real Time PCR Detection Manufacturer and Supplier |Foregene

OEM/ODM Factory China Magnetic Beads Njira Nucleic Acid Rna Extraction Reagent Kits for Real Time PCR Kuzindikira

OEM/ODM Factory China Magnetic Beads Method Nucleic Acid Rna Extraction Reagent Kits for Real Time Kuzindikira kwa PCR Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwamsanga DNA ya genomic kuchokera ku michira ya mbewa, makutu, minofu ndi zitsanzo zina zamtundu wa PCR, kotero ndizoyenera kwambiri kuyesa ma genetic.

Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65 ° C.Palibe njira zina monga kuchotsedwa kwa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2 × M1-PCR EasyTM Mix (UNG) imagwiritsa ntchito dUTP mmalo mwa dTTP pamaziko a 2 × M1-PCR EasyTM Mix (UNG), ndikuwonjezera UNG enzyme (Uracil-N-glycosylase) yomwe ingawononge template yomwe ili ndi dUTP nthawi yomweyo.PCR isanachitike, puloteni ya UNG imagwiritsidwa ntchito kusokoneza mankhwala a PCR okhala ndi uracil.Enzyme ya UNG sidzakhala ndi zotsatirapo pa template yomwe ilibe uracil, potero imatsimikizira kutsimikizika ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesa kwakukulu kwa majini.Kuyipitsidwa kwa zinthu za PCR kunachitika.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Bizinesi yathu imatsatira mfundo ya "Quality ikhoza kukhala moyo ndi kampaniyo, ndipo mbiri idzakhala moyo wake" kwa OEM/ODM Factory China Magnetic Beads Method Nucleic Acid Rna Extraction Reagent Kits for Real Time PCR Detection, Ogwira ntchito athu ali ndi cholinga chopereka mayankho ndi chiwongolero chamitengo yokwera ku zomwe tikuyembekezera, komanso kukhutitsa makasitomala athu kulikonse.
Bizinesi yathu imatsatira mfundo yofunikira ya "Ubwino ukhoza kukhala moyo ndi kampaniyo, ndipo mbiri ndiyomwe idzakhala moyo wake"Automatic Nucleic Acid Kuyeretsa, China Universal Rna DNA Reagents, Masiku ano zinthu zathu zimagulitsidwa padziko lonse lapansi ndi kunja chifukwa cha chithandizo chanthawi zonse komanso chatsopano chamakasitomala.Timapereka mankhwala apamwamba kwambiri komanso mtengo wampikisano, landirani makasitomala okhazikika komanso atsopano omwe amagwirizana nafe!

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	Buffer MP
	Foregene Protease Plus
	6 × DNA Loading Buffer
Gawo II	2 × M1 PCR Yosavuta^TMMix(UNG)
Malangizo

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

■ Zofuna zachitsanzo ndizochepa, momwe minofu ya mbewa ya 5mg kapena 2-5mm mchira wa mbewa ingayesedwe.

■ Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo opaleshoniyo ndi yosavuta.

■ The wokometsedwa PCR dongosolo zimapangitsa PCR kukhala apamwamba enieni ndi kupirira mwamphamvu PCR reaction inhibitors.

■ Anti-kuipitsa PCR dongosolo 2 × M1-PCR Easy^TMMix (UNG), yomwe imathetsa bwino kuipitsidwa ndi zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Zosintha za Kit

Kuchuluka kwa ntchito: mchira wa mbewa, khutu la mbewa, minofu ndi minofu ina.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

wk flw-mbewa mchira wolunjika PCR

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer AL ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

Reagent Foregene Protease ili ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

Reagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

Reagent 2 × M1 PCR Easy TM Mix (UNG), ngati imagwiritsidwa ntchito kawirikawiri, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (kugwiritsa ntchito mkati mwa masiku 10) .Bizinesi yathu imamatira pa mfundo yaikulu ya "Quality ikhoza kukhala moyo ndi olimba, ndipo mbiri yodziwika idzakhala moyo wake" kwa OEM / ODM Factory China Magnetic Beads Mamembala a PC Reaid Reaid Reid Rousation ndi Ogwira Ntchito Zathu Zowona za PC Zowonongeka Zowonongeka Zowonongeka, Mamembala Athu a PC Reid Reid Ration Excension cholinga chopereka mayankho okhala ndi mtengo wokwera wa magwiridwe antchito ku zomwe tikuyembekezera, komanso zomwe tikufuna tonsefe ndikukhutiritsa makasitomala athu padziko lonse lapansi.
OEM / ODM FactoryChina Universal Rna DNA Reagents, Automatic Nucleic Acid Kuyeretsa, Masiku ano zinthu zathu zimagulitsidwa padziko lonse lapansi ndi kunja chifukwa cha chithandizo chanthawi zonse komanso chatsopano chamakasitomala.Timapereka mankhwala apamwamba kwambiri komanso mtengo wampikisano, landirani makasitomala okhazikika komanso atsopano omwe amagwirizana nafe!

Zam'mbuyo: ODM Factory China Mtt Detection Kit 1000t Cell Viability Detection Reagent

Ena: OEM/ODM Factory China dNTP MIX (10mM)

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