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Imodzi mwazotentha kwambiri ku China Vtm Factory Direct Selling Virus Zitsanzo Zotolera Zitsanzo Zotayika

Imodzi mwazinthu zotentha kwambiri ku China Vtm Factory Direct Selling Virus Zitsanzo Zotolera Zitsanzo Zotayidwa za Sampling Tube Chithunzi Chowonetsedwa
  • Imodzi mwazotentha kwambiri ku China Vtm Factory Direct Selling Virus Zitsanzo Zotolera Zitsanzo Zotayika
  • Imodzi mwazotentha kwambiri ku China Vtm Factory Direct Selling Virus Zitsanzo Zotolera Zitsanzo Zotayika
  • Imodzi mwazotentha kwambiri ku China Vtm Factory Direct Selling Virus Zitsanzo Zotolera Zitsanzo Zotayika

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwachangu ma DNA amtundu wa nyama pazochitika za PCR, kotero ndiyoyenera kuyesa kwakukulu kwa majini.

Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65.°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2×PCR EasyTMMix (UNG) amagwiritsa ntchito dUTP m'malo mwa dTTP pamaziko a 2×PCR EasyTMSakanizani, ndikuwonjezera UNG enzyme (Uracil-N-glycosylase) yomwe ingawononge template yomwe ili ndi dUTP nthawi yomweyo.PCR isanachitike, puloteni ya UNG imagwiritsidwa ntchito kusokoneza mankhwala a PCR okhala ndi uracil.Enzyme ya UNG sidzakhala ndi zotsatira pa template yomwe ilibe uracil, potero imatsimikizira kutsimikizika ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuipitsidwa kwa zinthu za PCR pakuyesa kwakukulu kwa majini.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Cholinga chathu chachikulu chidzakhala kupatsa makasitomala ubale wamabizinesi ang'onoang'ono ofunikira komanso odalirika, kupereka chidwi chamunthu aliyense payekhapayekha kwa One of Hottest for China Vtm Factory Direct Selling Virus Sample Collection Disposable Sampling Tube, Pakadali pano, kampaniyo ili ndi mitundu yopitilira 4000 yazinthu ndipo idapeza mbiri yabwino komanso magawo akulu pamsika wapano ndi kunja.
Cholinga chathu chachikulu chidzakhala kupatsa makasitomala athu ubale wodalirika komanso wodalirika wamabizinesi ang'onoang'ono, kupereka chisamaliro chaumwini kwa iwo onse.China Disposable Sampling Tube, Sampling Collection Tube, Tikufuna kwambiri mwayi wochita bizinesi nanu komanso kukhala okondwa kuyika zambiri zamalonda athu.Ubwino wabwino kwambiri, mitengo yampikisano, kutumiza nthawi ndi ntchito zodalirika zitha kutsimikizika.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I

Bafa AL

Foregene Protease

6 × DNA Loading Buffer

Gawo II

2 × PCR YosavutaTMMix(UNG)

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo

■ Kufuna kwachitsanzo ndikochepa, monga momwe minofu yanyama ya 5mg ingayesedwe.

■ Palibe chithandizo chapadera monga kugaya ndi kuphwanya chofunika, ndipo opaleshoniyo ndi yosavuta.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

■ Anti-kuipitsa PCR dongosolo 2 × PCR EasyTMMix (UNG) imathetsa bwino kuipitsidwa kwa zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Zosintha za Kit

Ntchito: zosiyanasiyana nyama zimakhala.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

wk flw-nyama minofu yolunjika PCR

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer AL ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

Reagent Foregene Protease ili ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

Reagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

Reagent 2 × PCR YosavutaTMMix(UNG), ngati imagwiritsidwa ntchito pafupipafupi, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi (kugwiritsa ntchito mkati mwa masiku 10) .Cholinga chathu chachikulu chidzakhala kupatsa makasitomala athu ubale wabizinesi yaying'ono komanso wodalirika, kupereka chisamaliro chapadera kwa iwo onse kwa One of Hottest for China Vtm Factory Direct Selling Virus Zitsanzo Zosonkhanitsira Zitsanzo Zosonkhanitsira Zitsanzo Zotayidwa, Machubu Omwe Amagulitsidwa Oposa 0, Machubu Omwe Amagulitsidwa Oposa 0, Machubu Omwe Amagulitsidwa Oposa 0, Machubu Omwe Amagulitsidwa Pakalipano 0. udindo ndi magawo akuluakulu pamsika wamakono wapakhomo ndi kunja.
Chimodzi mwa Hottest kwaChina Disposable Sampling Tube, Sampling Collection Tube, Tikufuna kwambiri mwayi wochita bizinesi nanu komanso kukhala okondwa kuyika zambiri zamalonda athu.Ubwino wabwino kwambiri, mitengo yampikisano, kutumiza nthawi ndi ntchito zodalirika zitha kutsimikizika.

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