Ntchito yathu iyenera kukhala yotumizira ogwiritsa ntchito ndi ogula zinthu zapamwamba kwambiri komanso zopambana zonyamula katundu za digito ndi mayankho ake.PCR Reagent, Tikulandira ndi mtima wonse mabizinesi awiri akunja ndi apakhomo, ndipo tikukhulupirira kuti tidzagwira ntchito nanu pakapita nthawi!
Ntchito yathu iyenera kukhala yotumizira ogwiritsa ntchito ndi ogula zinthu zapamwamba kwambiri komanso zopambana zonyamula katundu za digito ndi mayankho ake.PCR Reagent, Pokhala ndi zaka pafupifupi 30 mubizinesi, takhala tikudalira ntchito zapamwamba, zabwino komanso kutumiza.Tikulandira mwachikondi makasitomala ochokera padziko lonse lapansi kuti agwirizane ndi kampani yathu pa chitukuko wamba.

Zofotokozera

5ml, 10ml, 40ml

Zida zamagulu

Mbali & ubwino

■ Kukhulupirika kwakukulu: kuwirikiza ka 6 kuposa ka enzyme wamba wa Taq;

■ Kuthamanga kwachangu kokulitsa : Kuthamanga kwa matalikidwe ndi 5-10sec/kb, yomwe ndi 6-12 nthawi ya Taq enzyme wamba;

■ Kusinthasintha kwa ma template: Imatha kukulitsa bwino ma templates osiyanasiyana ovuta a DNA ndi apamwambaZithunzi za GC;

■ Kuchulukitsitsa kwapamwamba: kuchepekera kwa kakulidwe kocheperako kuposa ma enzyme wamba a Taq;

■ Kulekerera kwa chilengedwe kumakhala kolimba: kuikidwa pa 37 ° C kwa sabata, kusunga ntchito yoposa 90%;

■ Ili ndi 5′ →3′ DNA polymerase ntchito ndi 5′→3’ exonuclease ntchito, popanda 3′ →5’ exonuclease ntchito.

■ Chidacho chimasakanikirana ndi Electrophoresis Loading Buffer, PCR itatha, chitsanzocho chikhoza kuikidwa mwachindunji kwa electrophoresis;ntchito ndi yosavuta komanso yosavuta.

Kit application

Kukulitsa kwa PCR kwa zidutswa za DNA, zilembo za DNA, kutsatizana kwa DNA, PCR kuphatikiza A mchira

Kusungirako ndi moyo wa alumali

Ikhoza kusungidwa kwa nthawi yaitali pa -20 ° C;kuti zigwiritsidwe ntchito kawirikawiri, zimatha kusungidwa pa 4 ° C kwa nthawi yochepa.The 2 × Real PCR EasyTM Mix-SYBR yoperekedwa ndi Real Time PCR EasyTM-SYBR Green I kit ndi dongosolo latsopano la premix lomwe limagwiritsa ntchito SYBR Green I kwa Real Time PCR amplification reactions, zomwe zingathe kusintha kwambiri tsatanetsatane wa mankhwala ndi kukhudzidwa kwa machitidwe .ROX imaperekedwa ngati utoto wowongolera mkati.Kuchuluka kwa fluorescence kwa zida izi ndi 3-5 kuwirikiza kawiri kwa zinthu zofanana, zomwe zimatha kukhala zovutirapo komanso kuwonetsa mwachilengedwe kuchuluka kwa template ya DNA.
2 × Real PCR EasyTM Mix-SYBR ili ndi mawonekedwe oyambira otentha a Foregene a Taq DNA Polymerase.Poyerekeza ndi ma enzyme wamba a Taq, ili ndi zabwino zake pakukweza kwakukulu, kuthekera kokulirapo kwamphamvu komanso kutsika kosagwirizana.Itha kuchepetsa kukulitsa kosakhazikika ndikuwongolera kulondola kwa PCR.