Nthawi zonse timaganiza ndikuchita mogwirizana ndi kusintha kwa zochitika, ndikukula.Tikufuna kukwaniritsa malingaliro ndi thupi lolemera komanso kukhala ndi moyo kwa Ogulitsa Maginito a Professional Magnetic Viral Rna Nucleic Acid Isolation Purification Prepacked Kits, Kampani yathu ikuchita ndi ndondomeko ya "umphumphu, mgwirizano womwe unapangidwa, anthu okhazikika, kupambana-kupambana mgwirizano".Tikukhulupirira kuti titha kukhala ndi mgwirizano wosangalatsa ndi wamalonda ochokera kuzungulira chilengedwe chonse.
Nthawi zonse timaganiza ndikuchita mogwirizana ndi kusintha kwa zochitika, ndikukula.Tikufuna kukwaniritsa malingaliro ndi thupi lolemera komanso kukhala ndi moyoChina Universal Rna DNA Reagents ndi Automatic Nucleic Acid Kuyeretsa, Tikufuna kukwaniritsa zofuna za makasitomala athu padziko lonse lapansi.Mitundu yathu yamalonda ndi ntchito zikukulirakulirabe kuti zikwaniritse zomwe makasitomala amafuna.Tikulandira makasitomala atsopano ndi akale ochokera m'mitundu yonse kuti atilumikizane ndi mabizinesi am'tsogolo ndikuchita bwino!
Mabuku a Malangizo:

 Nthawi zonse timaganiza ndikuchita mogwirizana ndi kusintha kwa zochitika, ndikukula.Tikufuna kukwaniritsa malingaliro ndi thupi lolemera komanso kukhala ndi moyo kwa Ogulitsa Maginito a Professional Magnetic Viral Rna Nucleic Acid Isolation Purification Prepacked Kits, Kampani yathu ikuchita ndi ndondomeko ya "umphumphu, mgwirizano womwe unapangidwa, anthu okhazikika, kupambana-kupambana mgwirizano".Tikukhulupirira kuti titha kukhala ndi mgwirizano wosangalatsa ndi wamalonda ochokera kuzungulira chilengedwe chonse.
Ogulitsa Magulu aChina Universal Rna DNA Reagents ndi Automatic Nucleic Acid Kuyeretsa, Tikufuna kukwaniritsa zofuna za makasitomala athu padziko lonse lapansi.Mitundu yathu yamalonda ndi ntchito zikukulirakulirabe kuti zikwaniritse zomwe makasitomala amafuna.Tikulandira makasitomala atsopano ndi akale ochokera m'mitundu yonse kuti atilumikizane ndi mabizinesi am'tsogolo ndikuchita bwino!

Vuto Kusanthula Buku

Kuwunika kotsatiraku kwamavuto omwe mungakumane nawoZomera ZonseRNA extraction will help you with your experiments. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali : Tech@foregene.com.

Mzere wozungulira watsekedwa

Kutsekeka kwa mzere wozungulira kumapangitsa kuti zokolola za RNA zichepe kapena kulephera kuyeretsedwa kuti mupeze RNA, ndipo mtundu wa RNA womwe wapezedwa udzakhala wotsika.

Kusanthula Zomwe Zimayambitsa:

1. Chitsanzo sichinaswe kwathunthu.

Kugawikana kosakwanira kwachitsanzo kumatha kuletsa DNA-Cleaning Column, yomwe ingakhudzenso zokolola za RNA ndi mtundu wake.Tikukulimbikitsani kuti mukamagawanitsa zitsanzo, perani mwachangu kuchuluka kwa nayitrogeni wamadzimadzi kuti muphwanye minyewa monga makoma a cell ndi nembanemba zama cell momwe mungathere.Pazitsanzo za mbewu za polyphenol polysaccharides, timalimbikitsa kuti mugwiritse ntchito Plant Total RNA Isolation Kit Plus.

2.Aspirate the DNA-Cleaning Column isolated supernatant, aspirate the zotheka cell zinyalala pellet.

Aspirated cell debris pellet imatha kutseka RNA-Oly Column panthawi ya RNA adsorption process (onani sitepe 5 ya ndondomeko, sitepe 6 ya polysaccharide polyphenol process).Tikukulimbikitsani kuti musamalire pamene mukulakalaka chodabwitsa ichi kuti mupewe zinyalala zama cell.

3. Chiwerengero choyambirira cha zitsanzo ndi chachikulu kwambiri.

Kugwiritsa ntchito kwambiri zitsanzo kumabweretsa kugawikana kosakwanira kwa ma cell kapena kusakwanira kwathunthu kwa maselo ndi Buffer PRL1 kapena Buffer PSL1, zomwe zimapangitsa kuti pakhale gawo lotsekeka loyeretsera ntchito zoyeretsa.Plant Total RNA Isolation Kit ili ndi kuchuluka koyambirira kwa 50 mg pa kuyeretsedwa kumodzi kwa zitsanzo zoyendetsedwa.Pazitsanzo za zomera za polyphenol polysaccharides, tikupangira kuti muyese Plant Total RNA Isolation Kit Plus.

4. Kutentha kwa centrifuge ndikotsika kwambiri.

Kudzipatula kwathunthu kwa RNA ndi kuyeretsedwa, kupatula kusokonezeka kwa nayitrogeni wamadzimadzi amtundu wa zitsanzo, masitepe onse amachitikira kutentha (20-25 ° C).Ma centrifuge ena otsika amakhala ndi kutentha kosachepera 20 °C, zomwe zingayambitse kutsekeka kwa DNA-Cleaning Column ndi/kapena RNA-Oly Column.Izi zikachitika, ikani kutentha kwa centrifuge ku 20-25 °C ndikutenthetsa chisakanizo cha lysis ndi/kapena kuwonjezera kwa ethanol kupatukana kwamphamvu kwambiri mpaka 37 °C.

Palibe RNA yotengedwa kapena zokolola za RNA ndizochepa

Nthawi zambiri pamakhala zinthu zambiri zomwe zimakhudza kuchira bwino, monga: zitsanzo za RNA, njira yogwirira ntchito, kuchuluka kwa elution, ndi zina zambiri.

Kusanthula kwazomwe zimayambitsa zomwe zili pansipa:

1.Kusambira kwa ayezi kapena kutentha kochepa (4 ° C) centrifugation kunachitika panthawi ya opaleshoni.

Malangizo: Gwiritsani ntchito kutentha kwapakati (15-25 ° C) panthawi yonseyi, musamasambitse madzi oundana komanso kutentha kwapakati.

       2.RNA yawonongeka chifukwa cha kusungidwa kosayenera kwa chitsanzo kapena kusungidwa kwa nthawi yaitali kwa chitsanzo.

Malangizo: Zitsanzo zomwe zangotengedwa kumene ziyenera kusungidwa mwachangu mu nayitrogeni wamadzimadzi, kenako kusungidwa pa -80 ° C kwa nthawi yayitali, kupewa kuzizira mobwerezabwereza ndi kusungunuka kwa zitsanzo;kapena nthawi yomweyo zilowerereni zitsanzo mu RNA stabilizer RNAlater solution (zitsanzo za nyama).

       3.Kugawika kwachitsanzo kosakwanira ndi lysis kumabweretsa kutsekeka kwa gawo loyeretsa.

Lingaliro: Mukamagaya minofu, chonde onetsetsani kuti minofuyo ili pansi mokwanira, ndipo mwamsanga mutumize ku Buffer PSL1 yokonzekera kale (tsimikizirani kuti gawo lolondola la β-ME lawonjezeredwa, onani sitepe 1 ya ndondomekoyi).

4.Chidziwitso chinawonjezedwa molakwika.

Yesani: Onetsetsani kuti RNase-Free ddH₂O amadonthozedwa mkatikati mwa nembanemba yoyeretsa.

5.Voliyumu yolondola ya ethanol mtheradi sanawonjezedwe ku Buffer PSL2 kapena Buffer PRW2.

Malingaliro: Chonde tsatirani malangizo, onjezani voliyumu yolondola ya ethanol ku Buffer PSL2 ndi Buffer PRW2 ndipo sakanizani bwino zida zisanagwiritsidwe.

6.Kuchuluka kwa chitsanzo cha minofu ndi kosayenera.

Malingaliro: Gwiritsani ntchito 50 mg ya minofu pa 500 μl ya Buffer PSL1.Kugwiritsa ntchito minofu yambiri kumachepetsa kuchuluka kwa RNA yotulutsidwa ndipo chiyero cha RNA chotsatira chidzachepetsedwanso.Tikukulimbikitsani kuti mlingo woyambirira wa chitsanzo usapitirire 50 mg pa ntchito yochotsa RNA.

7. Voliyumu yofotokozera molakwika kapena kusakwanira.

Lingaliro: Voliyumu yosadziwika ya gawo loyeretsera ndi 50-200 μl;ngati mphamvu ya elution siili yokhutiritsa, tikulimbikitsidwa kuwonjezera nthawi kutentha kwapakati mutatha kuwonjezera RNase-Free ddH₂O, monga 5-10min.

8.Mzere woyeretsedwa uli ndi zotsalira za ethanol mutatsuka ndi Buffer PRW2.

   Lingaliro: Ngati chubu chopanda kanthu chili ndi centrifuged kwa 1 min ndipo pali ethanol yotsalira mutatsuka mu Buffer PRW2, mukhoza kuwonjezera nthawi ya chubu chopanda kanthu centrifugation ku 2 min, kapena ikani ndime yoyeretsera kutentha kwa mphindi 5 kuti muchotse ethanol yotsalira.

9.Chidacho chinagwiritsidwa ntchito molakwika.

   Malingaliro: Pazitsanzo za mbewu za polyphenolic polysaccharides, kugwiritsa ntchito zida wamba monga Plant Total RNA Isolation Kit sangathe kupeza zitsanzo zabwino za RNA.Tikukulangizani kuti mugwiritse ntchito Plant Total RNA IsolationKit Plus, yomwe idapangidwira mwapadera zitsanzo za mbewu za polyphenolic polysaccharide.Chida chopangidwa mwapadera chochotsera RNA kuchokera ku zitsanzo za mbewu za polyphenol ndi polysaccharide.

Mtengo wa OD260/OD280 ndiwotsika

Kusintha kwa RNA ndi ddH₂O ndi kugwiritsidwa ntchito powerengera ma spectrophotometer kumapangitsa kuti OD260/OD280 ikhale yotsika.Timalimbikitsa kugwiritsa ntchito 10 mM Tris-HCl, pH 7.5 (m'malo mwa RNase-Free ddH₂O to elute RNA) kuti mupeze mfundo zolondola za OD260/OD280, onani “RNA Concentration and Purification Assays” patsamba 19.

RNA yoyeretsedwa ndiyonyozeka

Ubwino wa RNA yoyeretsedwa umagwirizana ndi zinthu monga kusungidwa kwa zitsanzo, kuipitsidwa kwa RNase, ndi kusintha.

Kusanthula kwazomwe zimayambitsa:

Zitsanzo za 1.Tissue sizinasungidwe mu nthawi pambuyo pa kusonkhanitsa.

    Malangizo: Ngati zitsanzo za minofu sizikugwiritsidwa ntchito pakapita nthawi, chonde zisungeni mu nayitrogeni wamadzimadzi pa kutentha kochepa kapena kuwasamutsira ku -80 ° C kuti asungidwe kwa nthawi yaitali mutatha kuzizira mofulumira mu nayitrogeni wamadzimadzi, kapena kumiza mwamsanga zitsanzo mu RNA stabilizer RNAlater solution (zitsanzo za nyama).Pochotsa RNA, yesani kugwiritsa ntchito zitsanzo za minofu zomwe zasonkhanitsidwa mwatsopano.

2.Kuzizira kobwerezabwereza ndi kusungunuka kwa zitsanzo za minofu.

   Yesani izi: Posunga zitsanzo za minofu, ndi bwino kuziduladula kuti zisungidwe, ndi kuchotsamo mbali ina yake pozigwiritsira ntchito kupeŵa kuwonongeka kwa RNA kochititsidwa ndi kuzizira kobwerezabwereza ndi kusungunuka kwa zitsanzozo.

3.RNase imayambitsidwa mchipinda chogwirira ntchito kapena osavala magolovesi otaya, masks, ndi zina.

   Lingaliro: Kuyesa kwa RNA m'zigawo kumachitidwa bwino pamachitidwe osiyanasiyana a RNA, ndipo tebulo la labotale liyenera kutsukidwa kuyesako kusanachitike, ndipo magolovesi otayika ndi masks ayenera kuvalidwa panthawi yoyesera kuti apewe kuwonongeka kwa RNA komwe kumachitika chifukwa cha kukhazikitsidwa kwa RNase kwambiri.

4.The reagent imakhudzidwa ndi RNase panthawi yogwiritsira ntchito.

   Yesani: M'malo mwake lowetsani zida zatsopano za RNA zotulutsira mbewu pazoyeserera zofananira.

5.Machubu a centrifuge ndi malangizo a pipette omwe amagwiritsidwa ntchito pakusintha kwa RNA ali ndi kachilombo ka RNase.

Malangizo: Onetsetsani kuti machubu a centrifuge, nsonga za pipette, pipettes, ndi zina zotero zomwe zimagwiritsidwa ntchito pochotsa RNA zonse ndi RNase-Free.

Mabuku a Malangizo:

Bzalani Total RNA Isolation Kit Instruction Manual