Magazi a DNA Mini Kit
Kufotokozera Kwa Zida:
Yeretsani mwachangu DNA yapamwamba kwambiri ya genomic kuchokera m'magazi a anticoagulated (<1ml).
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Mafotokozedwe a Kit
Chidachi chimagwiritsa ntchito makina atsopano a Foregene Protease Plus ndi ma buffer apadera a BL1 ndi BL2, omwe amatha kugaya magazi a anticoagulant m'kanthawi kochepa, motero kupewa kuwonongeka kwa DNA mpaka kufika pamlingo waukulu ndikupeza kuchuluka kwa ma genomic DNA.Njira yopangira magazi mwachangu komanso ntchito yosavuta ya DNA-Only ndime imathandizira kwambiri kutulutsa kwamtundu wamagazi, kupanga DNA ya genomic ikhoza kupezeka mwapamwamba komanso yoyera kwambiri mkati mwa mphindi 40.
DNA-yokha silica gel nembanemba ntchito pa centrifuge ndime ya zida izi ndi mtundu watsopano wa zinthu wapadera kwa kampani yathu, amene angathe kumanga DNA efficiently ndi mwachindunji, ndipo akhoza kuchotsa RNA, zosafunika, mapuloteni, ayoni ndi zina organic mankhwala m'maselo pazipita.Zidutswa za DNA zomwe zapezedwa ndi zazikulu, zoyera kwambiri, zokhazikika komanso zodalirika.Kulemera kwakukulu kwa gawo la DNA-Only ndi 80 μg DNA.DNA yomwe yapezeka ingagwiritsidwe ntchito poyesa ma cell biology monga digestion ya enzyme, PCR, hybridization yakumwera ndi kumanga laibulale.
Chidacho chimatha kukonza mpaka 1ml ya magazi nthawi imodzi kuti ipeze kuchuluka kokwanira kwa DNA yoyera kwambiri ya genomic.
Zofotokozera
| Magazi a DNA Mini Kit | |||
| Zamkatimu | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Mtengo wa BL1 | 15 ml pa | 30 ml | 75 ml pa |
| Bafa BL2 * | 15 ml pa | 30 ml | 75 ml pa |
| Buffer DC | 100 ml | 200 ml | 500 ml |
| PW yamphamvu * | 25 ml pa | 50 ml | 125 ml |
| Mtengo WB1 | 15 ml pa | 30 ml | 75 ml pa |
| Mtengo wa EB | 10 ml pa | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 mL | 2.5 ml | 6.5 ml |
| Nambala ya DNA Yokha | 50 | 100 | 250 |
| Pamanja | 1 | 1 | 1 |
*: Buffer BL2 ndi Buffer PW ali ndi mchere wamadzimadzi okwiyitsa.Chonde valani magolovesi ndikuchitapo kanthu zodzitetezera mukamagwira ntchito.
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.
-Kuthamanga Kwambiri: Foregene Protease Plus ili ndi ntchito yapamwamba kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mofulumira;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa ma genomic DNA imatha kutha mkati mwa mphindi 40.
-Kuthandizira: The centrifugation ikuchitika kutentha kwa firiji, 4 ° C centrifugation kapena ethanol mpweya wa DNA sikufunika.
-Safety: Palibe organic reagent m'zigawo zomwe zimagwiritsidwa ntchito.
-Zapamwamba: Zidutswa za DNA zomwe zatulutsidwa ndi zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira pazoyeserera zosiyanasiyana.
Kit application
Ndiwoyenera kuchotsa ndi kuyeretsa DNA ya genomic kuchokera kumagazi atsopano kapena oundana omwe ali ndi anticoagulated.
Kuyenda kwa ntchito
Kusungirako ndi moyo wa alumali
Chidachi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa pa 2-8 ° C.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa firiji kwakanthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
Njira yothetsera Foregene Protease Plus ili ndi njira yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);idzakhala yogwira ntchito komanso yokhazikika ikasungidwa pa 4 ° C, choncho ndi bwino kuti muisunge pa 4 ° C, kumbukirani kuti musaisunge pa -20 ° C.
