chifukwa cha kampani yabwino kwambiri, mitundu yosiyanasiyana yazogulitsa, zolipiritsa zampikisano komanso kutumiza bwino, timasangalala ndi mbiri yabwino kwambiri pakati pa makasitomala athu.Takhala gulu lamphamvu lomwe lili ndi msika waukulu waku China Gold Supplier ku China Zitsanzo Zaulere 32t 64t 96t DNA Rna Nucleic Acid Isolation Reagent Kit From Original Manufacturer Uni-Medica yokhala ndi CE ndi FDA, Tikulandila mwayi wochita bizinesi nanu ndipo tikuyembekeza kukhala osangalala kuyika tsatanetsatane wazogulitsa zathu.
chifukwa cha kampani yabwino kwambiri, mitundu yosiyanasiyana yazogulitsa, zolipiritsa zampikisano komanso kutumiza bwino, timasangalala ndi mbiri yabwino kwambiri pakati pa makasitomala athu.Takhala gulu lachangu lomwe lili ndi msika waukuluChina Nucleic Acid Isolation Reagent, Nucleic Acid Extraction Reagent, Ndi malonda apamwamba kwambiri, ntchito zabwino zogulitsa pambuyo pogulitsa ndi ndondomeko ya chitsimikizo, timapeza chidaliro kuchokera kwa anzathu ambiri akunja, ndemanga zambiri zabwino zawona kukula kwa fakitale yathu.Ndi chidaliro chonse ndi mphamvu, kulandira makasitomala kulankhula ndi kudzacheza nafe ubale tsogolo.

Mafotokozedwe a Kits

50 Preps, 200 Preps

Chidachi chimagwiritsa ntchito spin column ndi formula yopangidwa ndi kampani yathu, yomwe imatha kuchotsa chiyero chapamwamba komanso chapamwamba kwambiri cha RNA kuchokera kumagulu osiyanasiyana a nyama ndikuchita bwino kwambiri.Imapereka DNA-Cleaning Column yogwira mtima, yomwe imatha kulekanitsa ndi kutsatsa DNA ya genomic kuchokera ku supernatant ndi minofu lysate, yosavuta komanso yopulumutsa nthawi;Mzere wa RNA wokha ukhoza kumangirira RNA bwino ndipo ukhoza kusinthidwa nthawi imodzi ndi chilinganizo chapadera Zitsanzo zambiri.

Dongosolo lonselo ndi RNase-Free, kotero kuti RNA yochotsedwayo isawonongeke;Buffer RW1, Buffer RW2 buffer makina ochapira, kotero kuti RNA yopezekayo ilibe mapuloteni, DNA, ion, ndi kuipitsidwa kwapawiri.

Zida za zida:

Mbali & ubwino

■ Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA;dongosolo lonse ndi RNase-Free
■ Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column
■ Chotsani DNA popanda kuwonjezera DNase
■ Zosavuta-zochita zonse zimatsirizidwa pa kutentha kwa chipinda
■ Kuthamanga kwachangu kumatha kutha mphindi 30
■ Safe-palibe organic reagent chofunika
■ Kuyera kwakukulu -OD260/280≈1.8-2.1

Kit application

Ndiwoyenera kuchotsedwa ndi kuyeretsedwa kwa RNA yonse kuchokera kumitundu yosiyanasiyana yanyama yatsopano kapena yozizira kapena maselo otukuka.

Zogulitsa katundu

■ Ntchito zotsika: kaphatikizidwe ka cDNA yoyamba, RT-PCR, kupanga maselo, Northern Blot, etc.
■ Zitsanzo: minofu ya nyama, maselo otukuka
■ Mlingo: Tissues 10-20mg, Maselo(2-5)×10⁶
■ Kuchuluka kwa DNA kumangiriza mphamvu yoyeretsa: 80 μg
■ Voliyumu yowonjezera: 50-200 μl

Kuyenda kwa ntchito

Chithunzi

Animal Total RNA Isolation Kit ankachitira 20mg
Zitsanzo za mbewa zatsopano, tengani 5% oyeretsedwa okwana RNA 1% agar

Glycogel electrophoresis
1: Nkhumba 2: Impso
3: Chiwindi 4: Mtima

Kusungirako ndi moyo wa alumali

Zidazi zitha kusungidwa kwa miyezi 24 kutentha (15-25 ℃) kapena 2-8 ℃ kwa nthawi yayitali.Buffer RL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi wa 1 mutawonjezera β- mercaptoethanol (posankha).Takhala gulu lamphamvu lomwe lili ndi msika waukulu waku China Gold Supplier ku China Zitsanzo Zaulere 32t 64t 96t DNA Rna Nucleic Acid Isolation Reagent Kit From Original Manufacturer Uni-Medica yokhala ndi CE ndi FDA, Tikulandila mwayi wochita bizinesi nanu ndipo tikuyembekeza kukhala osangalala kuyika tsatanetsatane wazogulitsa zathu.
China Gold Supplier kwaChina Nucleic Acid Isolation Reagent, Nucleic Acid Extraction Reagent, Ndi malonda apamwamba kwambiri, ntchito zabwino zogulitsa pambuyo pogulitsa ndi ndondomeko ya chitsimikizo, timapeza chidaliro kuchokera kwa anzathu ambiri akunja, ndemanga zambiri zabwino zawona kukula kwa fakitale yathu.Ndi chidaliro chonse ndi mphamvu, kulandira makasitomala kulankhula ndi kudzacheza nafe ubale tsogolo.

RNA sinachotsedwe kapena zokolola za RNA ndizochepa

Nthawi zambiri pamakhala zinthu zosiyanasiyana zomwe zimakhudza kuchira bwino, monga: minofu ya RNA yokhutira, njira yogwirira ntchito, voliyumu ya elution, ndi zina zambiri.

1. Kusambira kwa ayezi kapena cryogenic (4 ° C) centrifugation kunachitika panthawi ya ntchito.

Malangizo: Gwiritsani ntchito kutentha (15-25 ° C) panthawi yonseyi, osasamba madzi oundana ndi centrifuge pa kutentha kochepa.

2. Kusungidwa kwachitsanzo kosayenera kapena nthawi yochuluka yosungira zitsanzo.

Malangizo: Sungani zitsanzo pa -80 ° C kapena kuzizira mu nayitrogeni wamadzimadzi ndipo pewani kugwiritsa ntchito kuzizira kobwerezabwereza;yesetsani kugwiritsa ntchito minofu yatsopano kapena maselo otukuka kuti muchotse RNA.

3. Kusakwanira kwachitsanzo lysis.

Malangizo: Mukamagwirizanitsa minofu, onetsetsani kuti minofuyo ili ndi homogenized mokwanira komanso kuti maselo a minofu agawanika mokwanira kuti afotokoze kutulutsidwa kwa RNA.

4. Chosowacho sichiwonjezedwa bwino.

Malangizo: Tsimikizirani kuti RNase-Free ddH₂O amawonjezedwa pang'onopang'ono pakati pa nembanemba yoyeretsa.

5. Voliyumu yolondola ya ethanol yokwanira sinawonjezedwe ku Buffer RL2 kapena Buffer RW2.

Malangizo: Tsatirani malangizo, onjezani voliyumu yolondola ya ethanol ku Buffer RL2 ndi Buffer RW2 ndikusakaniza bwino musanagwiritse ntchito zida.

6. Mlingo wa zitsanzo za minofu siwoyenera.

Malangizo: Gwiritsani ntchito 10-20 mg ya minofu kapena (1-5) × 10⁶ma cell pa 500 μl buffer RL1, chifukwa kugwiritsa ntchito minofu mopitirira muyeso kungayambitse kuchepa kwa RNA.

7. Voliyumu yolakwika kapena kusakwanira.

Malangizo: Voliyumu ya elution ya gawo loyeretsa ndi 50-200 μl;ngati mphamvu ya elution siili yokhutiritsa, tikulimbikitsidwa kuwonjezera nthawi yoyika kutentha kwa chipinda mutawonjezera RNase-Free ddH yotenthetsera.₂O, mwachitsanzo kwa 5-10 min.

8.Mzere woyeretsedwa uli ndi zotsalira za ethanol pambuyo pa kusamba kwa Buffer RW2.

Malangizo: Ngati pali zotsalira za ethanol pambuyo pa kusamba kwa Buffer RW2, chubu chopanda kanthu centrifugation kwa 1min, nthawi yopanda kanthu chubu centrifugation ntchito akhoza ziwonjezeke kwa 2min, kapena ndime kuyeretsa akhoza kuikidwa pa firiji kwa mphindi 5 mokwanira kuchotsa Mowa yotsalira.

RNA yoyeretsedwa ndiyonyozeka

Ubwino wa RNA yoyeretsedwa ikugwirizana ndi zinthu monga kusungidwa kwa chitsanzo, kuipitsidwa kwa RNase, ndi kusintha, ndi zina zotero.

1. Zitsanzo za minofu sizimasungidwa nthawi.

Malangizo: Ngati zitsanzo za minofu kapena maselo sagwiritsidwa ntchito munthawi yake mutatolera, nthawi yomweyo sungani pa -80 °C kapena nayitrogeni wamadzimadzi.Kuti mutulutse RNA, gwiritsani ntchito minofu yongotengedwa kumene kapena maselo a cell ngati kuli kotheka.

2. Kuzizira kobwerezabwereza kwa zitsanzo za minofu.

Malangizo: Posunga zitsanzo za minofu, ndi bwino kuzidula kuti zisungidwe, ndikuchotsa chimodzi mwa zidutswazo pozigwiritsira ntchito kuti musamaundane mobwerezabwereza ndi kuwonongeka kwa RNA.

3. RNase imayambitsidwa kapena kusavala magolovesi otayika, masks, ndi zina zotero panthawi ya opaleshoni.

Malangizo: Kuyesa kwa RNA m'zigawo kumachitika bwino m'zipinda zosinthira za RNA ndipo tebulo limachotsedwa kuyesako kusanachitike.

Valani magolovesi ndi masks otayika panthawi yoyesera kuti muchepetse kuwonongeka kwa RNA komwe kumachitika chifukwa cha kukhazikitsidwa kwa RNase.

4. Ma reagents amadetsedwa ndi RNase pakugwiritsa ntchito.

Malangizo: M'malo ndi Animal Total RNA Isolation Kit yatsopano yoyeserera yofananira.

5. Machubu a centrifuge, nsonga, ndi zina zambiri zomwe zimagwiritsidwa ntchito posokoneza RNA ndizoipitsidwa ndi RNase.

Malangizo: Tsimikizirani kuti machubu a centrifuge, nsonga, mapaipi, ndi zina zomwe zimagwiritsidwa ntchito pochotsa RNA zonse ndi Zaulere za RNase.

Oyeretsedwa analandira RNA amakhudza zoyesera kumunsi

RNA yoyeretsedwa ndi ndime yoyeretsera, ngati ayoni amchere, zomanga thupi ndi zazikulu kwambiri zidzakhudza kuyesera kumtunda, monga: reverse transcription,Northern Blot et al.

1. The elutioned RNA ili ndi zotsalira za ayoni zamchere.

Malangizo: Tsimikizirani kuti voliyumu yolondola ya ethanol yawonjezedwa ku Buffer RW2 ndikuchita ndime zoyeretsera 2 pa liwiro la centrifugal lomwe lasonyezedwa kuti ligwire ntchito;ngati pali zotsalira za mchere wa ion, siyani mzere woyeretsera ku Buffer RW2 kwa mphindi 5 pa kutentha kwapakati ndikuchita centrifugation kuti muwonjezere kuchotsa kuipitsidwa kwa mchere.

2. Ethanol zotsalira mu elutioned RNA.

Malangizo: Tsimikizirani kuti mutatha kutsuka kwa bafa RW2, gwiritsani ntchito chubu cha centrifugation chopanda kanthu pa liwiro la centrifugation lomwe likuwonetsedwa kuti ligwire ntchito, onjezerani nthawi ya ntchito yopanda kanthu ya chubu centrifugation mpaka 2 min ngati pali zotsalira za ethanol, kapena musiye kutentha kwa mphindi 5 pambuyo pa chubu chopanda kanthu centrifugation kuti muwonjezere kuchotsedwa kwa ethanol.