EndoFree Maxi Plasmid Kit (Spin Column)
Kufotokozera Kwa Zida:
Njira yonse yochotsa imatenga ola limodzi lokha, kuonetsetsa kuti ntchitoyo ndi yabwino komanso yachangu.
Kufotokozera
EndoFree Maxi Plasmid Kit imagwiritsa ntchito njira yapadera yotsatsira silika kuti imangirire DNA ya plasmid.Mwa kuphatikiza Endotoxin Removal Buffer P4 yapadera ndi EndoFree Maxi Filtration Columns, endotoxin, mapuloteni ndi zonyansa zina zitha kuchotsedwa bwino.Njira yonse yochotsa imatenga ola limodzi lokha, kuonetsetsa kuti ntchitoyo ndi yabwino komanso yachangu.
Kuchuluka kwa mabakiteriya ovomerezeka pa mchitidwe uliwonse: Pa plasmid yapamwamba kwambiri, tikulimbikitsidwa kugwiritsa ntchito 100 ml ya mabakiteriya culture media, ndipo zokolola zake zimakhala pafupifupi 500-1500 μg.Kwa plasmid yotsika kwambiri, 200 ml mabakiteriya culture media akulimbikitsidwa kuti apange pafupifupi 200-600 μg plasmids.
Zida zamagulu
| Zogulitsa Zigawo | D909-E(10 zokonzekera) |
| Mtengo wa BL | 30 ml |
| Mtengo P1 | 100 ml |
| Mtengo P2 | 100 ml |
| Mtengo P4 | 100 ml |
| Mtengo wa DW2 | 44 ml pa |
| Mtengo wa EB | 30 ml |
| RNase A(10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Columns | 10 ma PC |
| Sirinji Yosefera ya FinePure Maxi | 10 ma PC |
| 50 ml Machubu Otolera | 10 ma PC |
Kuzindikira kwa Plasmid DNA Concentration and Purity
Kuyera ndi kuchuluka kwa plasmid yotengedwa DNA kumatha kuzindikirika ndi agarose gel electrophoresis ndi ultraviolet spectrophotometer.Mtengo wa OD260 wa 1 umafanana ndi pafupifupi 50 μg/ml ya DNA yazingwe ziwiri.Mtengo wa OD260/OD280 wa DNA yoyeretsedwa ya plasmid nthawi zambiri imakhala pakati pa 1.7-1.9.DNA yoyeretsedwa ya plasmid imatha kugwiritsidwa ntchito mwachindunji pakuyesa komwe kuli ndi zofunikira zachiyero, monga kutumiza ma cell kapena kuyesa kwa vivo.
Kit application
DNA ya plasmid yotengedwa ndi zidazi imatha kugwiritsidwa ntchito pazinthu zosiyanasiyana, kuphatikiza ma enzymatic digestion, PCR, kutsatizana, ligation, kusinthika komanso kupatsirana mitundu yosiyanasiyana ya maselo.
Kusungirako ndi moyo wa alumali
EndoFree Maxi Plasmid Kit ikhoza kusungidwa kutentha (15-25 ° C) pansi pauma kwa miyezi 12.Onjezani RNase A ku Buffer P1 musanagwiritse ntchito koyamba, sakanizani bwino ndikusunga pa 2-8°C.Kusakaniza kungathe kusungidwa mokhazikika kwa miyezi isanu ndi umodzi.
