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Foreasy Taq DNA Polymerase

Foreasy Taq DNA Polymerase Chithunzi Chowonetsedwa
  • Foreasy Taq DNA Polymerase

Kufotokozera Kwa Zida:

◮Kukhazikika kwakukulu: Enzyme ili ndi ntchito yoyambira yotentha.

◮Kukulitsa Mwachangu: 10 sec/kb.

◮Template yosinthika kwambiri: itha kugwiritsidwa ntchito kukulitsa bwino mtengo wa GC High, wovuta kukulitsa template ya DNA.

◮Kukhulupirika kolimba: wamba Taq Enzyme ka 6.

◮Kukhazikika kwamphamvu kwamafuta: Itha kuyikidwa pa 37 ° C kwa sabata ndikusunga ntchito yopitilira 90%.

mphamvu zam'tsogolo


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FAQ

Kufotokozera

Foreasy Taq DNA Polymerase ndi puloteni yatsopano ya Taq yowonetsedwa mu Escherichia coli engineering bacteria pogwiritsa ntchito ukadaulo wa gene recombination.Enzyme yokha imakhala ndi ntchito yoyambira yotentha ndipo imatha kugwiritsidwa ntchito pa PCR wamba ndi qPCR;ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, koma palibe 3'→ 5' exonuclease ntchito.

Zida zamagulu

Chigawo

 IM-01011 IM-01012 IM-01013
Foreasy Taq DNA Polymerase(5 U/μL)  5000 U (1 mL)  50 KU (10 mL)  500 KU (100 mL)
2 × Taq Reaction Buffer  25ml × 5  250mL × 5  500mL × 25

Mbali & ubwino

- Kukhazikika kwakukulu: Enzyme ili ndi ntchito yoyambira yotentha.

- Kukulitsa Mwachangu: 10 sec/kb .

- Template yosinthika kwambiri: itha kugwiritsidwa ntchito kukulitsa bwino mtengo wa GC High, mitundu yosiyanasiyana ya DNA yovuta kukulitsa.

- Kukhulupirika kolimba: wamba Taq Enzyme ka 6.

- Kukhazikika kwamphamvu kwamafuta: Itha kuyikidwa pa 37 ° C kwa sabata ndikusunga zoposa 90% ntchito

Kit application

Machitidwe osiyanasiyana a PCR/qPCR ndi machitidwe a PCR olunjika

Kukulitsa kwa PCR kwa zidutswa za DNA

Kulemba kwa DNA

DNA kutsatizana

PCR A-mchira

U Tanthauzo

1U: Kuchuluka kwa enzyme yofunikira kuphatikiza 10 nmol ya deoxynucleotides muzinthu zosasungunuka za asidi pogwiritsa ntchito activated salmon sperm DNA ngati template/primer kwa mphindi 30 pa 74°C.

Mkhalidwe Wochita

Kutentha Nthawi Kuzungulira
37°C 5 mins 1
94°C 5 mins 1
94°C 10 Mphindi  

35
60°C 10 Mphindi
72°C 20 sec/kb
72°C 2 mins 1

Kusungirako

-20 ± 5 °C kwa zaka 2 kapena -80 °C posungira nthawi yaitali.

  • Zam'mbuyo:
  • Ena:

    • Palibe zizindikiro za amplification

    1.Taq DNA Polymerase mu kit imataya ntchito yake chifukwa cha kusungirako kosayenera kapena kutha kwa zida.
    Malangizo: Tsimikizirani zosungirako zida;onjezaninso kuchuluka koyenera kwa Taq DNA Polymerase kudongosolo la PCR kapena mugule Real Time PCR Kit yatsopano kuti muyesere.

    2.Pali zoletsa zambiri za Taq DNA Polymerase mu template ya DNA.
    Yesani: Yeretsaninso template kapena kuchepetsa kuchuluka kwa template yomwe yagwiritsidwa ntchito.

    3.Kuwerengera kwa Mg2 + sikoyenera.
    Malangizo: Kuphatikizika kwa Mg2+ kwa2× Real PCR Mix yomwe timapereka ndi 3.5mM.Komabe, pazoyambira zina zapadera ndi ma templates, ndende ya Mg2 + ikhoza kukhala yokwezeka.Chifukwa chake, mutha kuwonjezera mwachindunji MgCl2 kuti mukwaniritse ndende ya Mg2+.Ndibwino kuti muwonjezere Mg2+ 0.5mM nthawi iliyonse kuti muwongolere.

    4.Kukulitsa kwa PCR sikuli koyenera, ndipo kutsatizana koyambirira kapena kusakanikirana sikuli koyenera.
    Malingaliro: tsimikizirani kulondola kwa mndandanda wa zoyambira ndipo choyambira sichinawonongeke;ngati chizindikiro chokulitsa sichili bwino, yesani kuchepetsa kutentha kwa annealing ndikusintha ndende yoyambira bwino.

    5.Kuchuluka kwa template ndi kochepa kwambiri kapena kochuluka.
    Malangizo: Chitani template ya linearization gradient dilution, ndikusankha template yomwe ili ndi PCR yabwino kwambiri pakuyesa kwa Real Time PCR.

    NTC ili ndi mtengo wokwera kwambiri wa fulorosenti

    1.Reagent kuipitsidwa chifukwa pa ntchito.
    Malangizo: M'malo ndi zoyeserera zatsopano za Real Time PCR.

    2.Kuwonongeka kunachitika panthawi yokonzekera machitidwe a PCR.
    Malangizo: Tengani njira zodzitetezera panthawi yogwira ntchito, monga: kuvala magolovesi a latex, kugwiritsa ntchito nsonga ya pipette yokhala ndi fyuluta, ndi zina zotero.

    3.Zoyambira zimawonongeka, ndipo kuwonongeka kwa zoyambira kumayambitsa kukulitsa kosagwirizana.
    Malingaliro: Gwiritsani ntchito electrophoresis ya SDS-PAGE kuti muwone ngati zoyambira zawonongeka, ndikusintha ndi zoyambira zatsopano zoyeserera za Real Time PCR.

    Primer dimer kapena matalikidwe osakhala enieni

    1.Kuwerengera kwa Mg2 + sikoyenera.
    Malangizo: Mg2 + ndende ya 2 × Real PCR EasyTM Mix yomwe timapereka ndi 3.5 mM.Komabe, pazoyambira zina zapadera ndi ma templates, ndende ya Mg2 + ikhoza kukhala yokwezeka.Chifukwa chake, mutha kuwonjezera mwachindunji MgCl2 kuti mukwaniritse ndende ya Mg2+.Ndibwino kuti muwonjezere Mg2+ 0.5mM nthawi iliyonse kuti muwongolere.

    2.The PCR annealing kutentha ndi otsika kwambiri.
    Yesani: Wonjezerani kutentha kwa PCR ndi 1 ℃ kapena 2 ℃ nthawi iliyonse.

    3.The PCR mankhwala ndi yaitali kwambiri.
    Malangizo: Kutalika kwa mankhwala a Real Time PCR kuyenera kukhala pakati pa 100-150bp, osapitirira 500bp.

    4.Zoyambira zimawonongeka, ndipo kuwonongeka kwa zoyambira kudzatsogolera ku maonekedwe a kukulitsa kwapadera.
    Malingaliro: Gwiritsani ntchito electrophoresis ya SDS-PAGE kuti muwone ngati zoyambira zawonongeka, ndikusintha ndi zoyambira zatsopano zoyeserera za Real Time PCR.

    5.Dongosolo la PCR ndi losayenera, kapena dongosolo ndi laling'ono kwambiri.
    Malingaliro: Makina a PCR ndi ochepa kwambiri apangitsa kuti kuzindikira kuchepe.Ndi bwino kugwiritsa ntchito kachitidwe kachitidwe kolimbikitsidwa ndi chida chochulukira cha PCR kuti muyambitsenso kuyesa kwa Real Time PCR.

    Kusabwerezabwereza kolakwika kwa kachulukidwe

    1.Chidacho sichikuyenda bwino.
    Lingaliro: Pakhoza kukhala zolakwika pakati pa dzenje lililonse la PCR la chida, zomwe zimapangitsa kuti pakhale kusabereka bwino pakuwongolera kutentha kapena kuzindikira.Chonde fufuzani molingana ndi malangizo a chida chofananira.

    2.Chiyerekezo cha chiyero sichili chabwino.
    Langizo: Zitsanzo zodetsedwa zipangitsa kuti kuyesako kusakhalenso bwino, komwe kumaphatikizapo chiyero cha template ndi zoyambira.Ndikwabwino kuyeretsanso template, ndipo zoyambira zimatsukidwa bwino ndi SDS-PAGE.

    3.Kukonzekera kwadongosolo la PCR ndi nthawi yosungira ndi yaitali kwambiri.
    Yesani: Gwiritsani ntchito makina a Real Time PCR poyesera PCR mukangokonzekera, ndipo musayisiye pambali kwa nthawi yayitali.

    4.Kukulitsa kwa PCR sikuli koyenera, ndipo kutsatizana koyambirira kapena kusakanikirana sikuli koyenera.
    Malingaliro: tsimikizirani kulondola kwa mndandanda wa zoyambira ndipo choyambira sichinawonongeke;ngati chizindikiro chokulitsa sichili bwino, yesani kuchepetsa kutentha kwa annealing ndikusintha ndende yoyambira bwino.

    5.Dongosolo la PCR ndi losayenera, kapena dongosolo ndi laling'ono kwambiri.
    Malingaliro: Makina a PCR ndi ochepa kwambiri apangitsa kuti kuzindikira kuchepe.Ndi bwino kugwiritsa ntchito kachitidwe kachitidwe kolimbikitsidwa ndi chida chochulukira cha PCR kuti muyambitsenso kuyesa kwa Real Time PCR.

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