Zitsanzo zaulere za China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2
Kufotokozera Kwa Zida:
◮Chida cha gawo limodzi chimapanga zolembera zosinthika ndi qPCR machitidwe awiri mu chubu yomweyo, zimangofunika kuwonjezera template RNA, zoyambira za PCR ndi RNase-Free ddH.2O.
◮Zida zimatha kusanthula mwachangu komanso moyenera ma virus a RNA kapena kufufuza RNA.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
◮RT-qPCR YosavutaTM(One Step) -SYBR Green I kit imabwera ndi utoto wamkati wa ROX, womwe ungagwiritsidwe ntchito kuthetsa zolakwika zakumbuyo ndi ma sign pakati pa zitsime, zomwe ndizosavuta kuti makasitomala azigwiritsa ntchito mitundu yosiyanasiyana ya zida za PCR.
Timakhulupilira mu: Kupanga nzeru ndi moyo wathu ndi mzimu.Wapamwamba ndi moyo wathu.Ogula ayenera kukhala ndi Mulungu wathu kwaulere zitsanzo za China High Sensitivity DNA/Rna One-Step Probe Rt-QpcrKit V2, Mkati mwazoyeserera zathu, tili ndi masitolo ambiri ku China ndipo mayankho athu adatamandidwa ndi ogula padziko lonse lapansi.Takulandirani ogula atsopano ndi achikale kuti mutilumikizane ndi mabizinesi okhalitsa.
Timakhulupilira mu: Kupanga nzeru ndi moyo wathu ndi mzimu.Wapamwamba ndi moyo wathu.Ogula ayenera kukhala ndi Mulungu wathuChina Taq DNA Polymerase, Qpcr, Kugwira ntchito molimbika kuti mupitilize kupita patsogolo, zatsopano mumakampani, yesetsani kuchita bizinesi yoyamba.Timayesa momwe tingathere kumanga chitsanzo cha kasamalidwe ka sayansi, kuphunzira zambiri zamaluso, kukhala ndi zida zotsogola zopangira ndi kupanga, kupanga zinthu zoyambira kuitana, mtengo wololera, ntchito zapamwamba kwambiri, kutumiza mwachangu, kukupatsani kulenga mtengo watsopano.
Zofotokozera
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Zida zamagulu
| Gawo I | Mtengo P1 |
| Mtengo P2 | |
| 6 × DNA Loading Buffer | |
| Gawo II | 2 × Leaf PCR YosavutaTMMix(UNG) |
Mbali & ubwino
■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Chosavuta-chitsanzo chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2 × Mix, kuchepetsa chitsanzo Mumakonda zolakwa ndi anachita dongosolo kukonzekera nthawi
■ Kukonzekera mwachangu template kumatha kumaliza mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50.
■ High throughput-lysis reaction akhoza kumalizidwa mu 96-chitsime PCR mbale
Zosintha za Kit
Kugwiritsa ntchito: chizindikiritso chosinthidwa ma genetic, genotyping, etc.
Chitsanzo: tsamba la mbewu
Mlingo: Diameter 2-3mm (njira yolunjika), m'mimba mwake 5-7mm (njira ya lysis)
Mtundu wozindikiridwa: chidutswa chandamale ≤1kb
Kuyenda kwa ntchito
Electropherogram
Masabata awiri a PCR Mix anapeza zotsatira pansi pa chikhalidwe cha PCR amplification product contamination.Timakhulupirira kuti: Innovation ndi moyo wathu ndi mzimu.Wapamwamba ndi moyo wathu.Ogula akuyenera kukhala nawo ndi Mulungu wathu Waulere kwa China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, Mkati mwazoyeserera zathu, tili ndi masitolo ambiri ku China ndipo mayankho athu adatamandidwa ndi ogula padziko lonse lapansi.Takulandirani ogula atsopano ndi achikale kuti mutilumikizane ndi mabizinesi okhalitsa.
Zitsanzo zaulere zaChina Taq DNA Polymerase, Qpcr, Kugwira ntchito molimbika kuti mupitilize kupita patsogolo, zatsopano mumakampani, yesetsani kuchita bizinesi yoyamba.Timayesa momwe tingathere kumanga chitsanzo cha kasamalidwe ka sayansi, kuphunzira zambiri zamaluso, kukhala ndi zida zotsogola zopangira ndi kupanga, kupanga zinthu zoyambira kuitana, mtengo wololera, ntchito zapamwamba kwambiri, kutumiza mwachangu, kukupatsani kulenga mtengo watsopano.
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