Zitsanzo Zabwino Zotulutsa Reagent Nucleic Acid Extraction-Free Vtm Kit Njira Yoyesera ya PCR
Kufotokozera Kwa Zida:
2 × PCR HeroTM Mix system ili ndi kulolerana kwakukulu kwa PCR inhibitors kuposa njira wamba ya PCR Mix, ndipo imatha kuthana ndi kukulitsa kwa PCR kwama tempulo osiyanasiyana ovuta.Dongosolo lapadera la machitidwe a Taq Hero komanso kuchita bwino kwambiri kwa Taq Hero kumapangitsa kuti machitidwe a PCR akhale ndi magwiridwe antchito apamwamba, kutsimikizika komanso chidwi.
Wodzipereka pakuwongolera khalidwe labwino komanso chisamaliro choganizira makasitomala, ogwira ntchito athu odziwa zambiri amakhalapo nthawi zonse kuti akambirane zomwe mukufuna ndikuwonetsetsa kukhutitsidwa kwamakasitomala a Good Quality Sample Release Reagent Nucleic Acid Extraction-Free Vtm Kit One Step PCR Test Method, Onetsetsani kuti mwakhala omasuka kulankhula nafe kuti tikonzekere.ndipo tikuganiza kuti tigawana zomwe tikuchita pazamalonda ndi ogulitsa athu onse.
Wodzipereka pakuwongolera bwino kwambiri komanso kusamalira makasitomala mwanzeru, antchito athu odziwa zambiri amakhalapo nthawi zonse kuti akambirane zomwe mukufuna ndikuwonetsetsa kukhutitsidwa kwamakasitomala.China Sample Release Kit ndi Gawo Limodzi, Tili ndi gulu lodzipatulira komanso lochita zankhanza, ndi nthambi zambiri, zoperekera makasitomala athu.Tikuyang'ana mabizinesi anthawi yayitali, ndikuwonetsetsa kuti ogulitsa athu apindula kwambiri pakanthawi kochepa komanso kwakanthawi.
Zofotokozera
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Zida zamagulu
| Gawo I | Mtengo P1 |
| Mtengo P2 | |
| 6 × DNA Loading Buffer | |
| Gawo II | 2 × Leaf PCR YosavutaTMMix(UNG) |
Mbali & ubwino
■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Chosavuta-chitsanzo chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2 × Mix, kuchepetsa chitsanzo Mumakonda zolakwa ndi anachita dongosolo kukonzekera nthawi
■ Kukonzekera mwachangu template kumatha kumaliza mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50.
■ High throughput-lysis reaction akhoza kumalizidwa mu 96-chitsime PCR mbale
Zosintha za Kit
Kugwiritsa ntchito: chizindikiritso chosinthidwa ma genetic, genotyping, etc.
Chitsanzo: tsamba la mbewu
Mlingo: Diameter 2-3mm (njira yolunjika), m'mimba mwake 5-7mm (njira ya lysis)
Mtundu wozindikiridwa: chidutswa chandamale ≤1kb
Kuyenda kwa ntchito
Electropherogram
Milungu iwiri ya PCR Mix zotsatira zodziwika pansi pa chikhalidwe cha PCR amplification product contamination.Odzipereka ku kuwongolera khalidwe labwino ndi chisamaliro choganizira makasitomala, ogwira ntchito athu odziwa zambiri amakhalapo nthawi zonse kuti akambirane zomwe mukufuna ndikuwonetsetsa kukhutitsidwa kwamakasitomala pa Good Quality Sample Release Reagent Nucleic Acid Extraction-Free Vtm Kit One Step PCR Test Method, Onetsetsani kuti mukubwera kuti mulankhule nafe popanda mtengo.ndipo tikuganiza kuti tigawana zomwe tikuchita pazamalonda ndi ogulitsa athu onse.
Ubwino Wabwino wa China Samle Release Kit ndi One Step, Tili ndi gulu lodzipereka komanso lochita zachiwawa, ndi nthambi zambiri, zomwe zimapatsa makasitomala athu.Tikuyang'ana mabizinesi anthawi yayitali, ndikuwonetsetsa kuti ogulitsa athu apindula kwambiri pakanthawi kochepa komanso kwakanthawi.
