China Cell-free DNA Isolation Kit wopanga ndi Supplier |Foregene

DNA Isolation Kit yopanda ma cell

Kufotokozera Kwa Zida:

Kudzipatula kwa cfDNA ku zitsanzo za plasma

Mbiri ya TQ01BT0050, TQ01BT0100

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Kufotokozera

DNA yopanda ma cell (cfDNA) ndi kachidutswa kakang'ono ka cfDNA kotulutsidwa kuchokera ku ma cell kupita m'magazi komwe kumatha kunyamula chidziwitso chachilengedwe kuchokera.zotupa, tizilombo toyambitsa matenda ndi fetus.Kusanthula kwa cfDNA kumapereka zolembera zazikulu zowunikira, kuzindikira ndi kuwunika momwe matenda akupitirasion.Pakadali pano, ukadaulo uwu wagwiritsidwa ntchito kwambiri pozindikira kusintha kwa chotupa, kuwongolera mankhwala osokoneza bongo komanso kuneneratu,kuzindikira kwa tizilombo toyambitsa matenda komanso kuwunika kwanthawi yayitali asanabadwe.

Ma nucleic acid apamwamba ndiye chitsimikizo cha kafukufuku wa genomics.Foregene amagwiritsa ntchito ukadaulo woyeretsa mikanda ya superparamagnetickupatsa ogwiritsa ntchito njira yovuta kwambiri yochotsera nucleic acid yomwe imakwaniritsa mosavuta kutsitsa kwa nucleic acid kwa otsika.kuganizira.Monga kutsimikiziridwa ndi masauzande ambiri azachipatala, mankhwalawa amatha kutulutsa cfDNA yapamwamba kwambiri kuchokera kumadzi amthupi opanda ma cellmonga plasma watsopano kapena wowuma, seramu, pleural fluid, ascites ndi cerebrospinal fluid pofufuza zasayansi ndi matenda a in-vitro diagnosticntchito.

The Foregene Cell-free DNA Isolation Kit idapangidwa kuti izilekanitsa DNA yopanda ma cell (cfDNA) ku plasma ya munthu, seramu, madzimadzi amthupi, kapena zitsanzo za mkodzo.Zidazi zimatha kudzipatula mwachangu komanso mosavuta cfDNA munjira zitatu: lysis / kumanga, kutsuka ndi kutulutsa.Imagwiritsa ntchito mikanda ya superparamagnetic yopangidwa mwapadera yokhala ndi njira yapadera ya lysis / yomangiriza kuti ipezenso ma nucleic acid ozungulira ndi ma hydrogen bonding ndi mphamvu zama electrostatic, popanda kutsatsa mapuloteni ndi zonyansa zina.Ndi yabwino kwa malo opangira makina opangira makina opangira makina pokonzekera kusanthula kulikonse pogwiritsa ntchito PCR kapena NGS.

Zamkatimu ndi Kusungirako

Table 1 Foregene Cell-free DNA Isolation Kit

Cmfundo	Rchidwi	Ndalama(25 T)	Ndalama(50 T)	Ndalama(100 T)	Storage
Bokosi 1	Bafa AL	42 mLx1 botolo	89 mLx1 botolo	189 mLx1 botolo	10-30 ° C
	Mtengo wapatali wa magawo AW1	38 mLx1 botolo	83 mLx1 botolo	165 mLx1 botolo	10-30 ° C
	Mtengo wapatali wa magawo AW2	15 mLx1 botolo	33 mLx1 botolo	66 mLx1 botolo	10-30 ° C
	Elution	3mLx1 botolo	6mLx1 botolo	12 mLx1 botolo	10-30 ° C
Bokosi2	Magnetic Mikanda A	3 .2 mLx1 botolo	6 .5mLx1 botolo	13 mLx1 botolo	2-8 ° C
Bokosi2	Proteinase K	5mLx1 botolo	10 .5 mLx1 botolo	21 mLx1 botolo	2-8 ° C

Ndemanga:

[1] Osasakaniza zigawo zamagulu osiyanasiyana a zida.

[2] Buffer AL ikhoza kupanga ma precipitates, omwe samakhudza ntchito yake.Ngati mvula ikuwoneka, chonde ikani botolo la reagent mu bafa lamadzi la 56 °C kwa 1 0 - 20 min mpaka mvula isungunuke.Kenako sakanizani bwino musanagwiritse ntchito.

[3] Osaundana Mikanda ya Magnetic A.

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Mbali & ubwino

-Mwachangu M'zigawo High DNA m'zigawo zokolola, chiyero chabwino, ogwira kuchotsa zonyansa ndi kunsi kwa mtsinje PCR inhibitors;

Flexible Sample IncorporationKutulutsa kosavuta kwa cfDNA kuchokera ku 0.5mL kupita ku 4mL zitsanzo zokhala ndi ma voliyumu otsika mpaka 20uL

Ntchito ZosiyanasiyanaKutsimikiziridwa ndi zitsanzo zachipatala, zopangidwira chotupa, matenda ndi matenda obadwa nawo asanabadwe ndi biopsy yamadzi

Thandizo lalikulu la Automated Platform Imagwirizana ndi machitidwe opangira ma automated, Tecan, Hamilton, PE ndi malo ena opangira ma nucleic acid.

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