PCR Hero ndi
Kufotokozera Kwa Zida:
◮Kukhulupirika kwakukulu: 6nthawi ya enzyme wamba ya Taq;
◮Mofulumira aliwiro lokulitsa:liwiro lokulitsa ndi 5-10sec/kb, lomwe ndi 6-12 nthawi ya enzyme wamba ya Taq;
◮Zambiri zosinthika za template: imatha kukulitsa bwino ma tempulo osiyanasiyana ovuta a DNA okhala ndi ma GC apamwamba komanso ovuta kukulitsa;
◮ZapamwambaKukulitsa luso: kuchuluka kwa machulukidwe am'mimba ndi otsika kuposa ma enzyme wamba a Taq;
◮Smotalika Kulekerera kwachilengedwe: imayikidwa pa 37 ° C kwa sabata, kusunga kuposa90%ntchito;
◮Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.
Mafotokozedwe a Kit
Izi zikuphatikiza m'badwo watsopano wapadera wa Foregene wa Taq——Forgene Taq Hero, dNTPs wokometsedwa, MgCl2, zotchingira zochita, PCR reaction enhancer, optimizer ndi stabilizer.2 × PCR HeroTMMix system ili ndi kulolerana kwakukulu kwa PCR inhibitors kuposa njira wamba ya PCR Mix, ndipo imatha kuthana ndi kukulitsa kwa PCR kwama tempulo osiyanasiyana ovuta.Dongosolo lapadera la machitidwe a Taq Hero komanso kuchita bwino kwambiri kwa Taq Hero kumapangitsa kuti machitidwe a PCR akhale ndi magwiridwe antchito apamwamba, kutsimikizika komanso chidwi.
Zofotokozera
Kit Series
PH-01013:2× PCR NgwaziTMSakanizani 1.7ml × 3
PH-01014:2× PCR NgwaziTMSakanizani 1.7ml × 6
PH-01015:2× PCR NgwaziTMSakanizani 1.7ml × 24
Zida zamagulu
2 × PCR HeroTMSakanizani: 0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8), 100mM KCl, 5mM MgCl2, zokhazikika zina ndi zowonjezera.
Mbali & ubwino
-Kukhulupirika kwakukulu:6nthawi ya enzyme wamba ya Taq;
- Mofulumira aliwiro lokulitsa:liwiro lokulitsa ndi 5-10sec/kb, lomwe ndi 6-12 nthawi ya enzyme wamba ya Taq;
-Zambiri zosinthika za template: imatha kukulitsa bwino ma tempulo osiyanasiyana ovuta a DNA okhala ndi ma GC apamwamba komanso ovuta kukulitsa;
- ZapamwambaKukulitsa luso: kuchuluka kwa machulukidwe am'mimba ndi otsika kuposa ma enzyme wamba a Taq;
-Smotalika Kulekerera kwachilengedwe: imayikidwa pa 37 ° C kwa sabata, kusunga kuposa90%ntchito;
-Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.
Kit application
- Kukulitsa kwa PCR kwa zidutswa za DNA
- Kulemba kwa DNA
- DNA kutsatizana
- PCR kuphatikiza A mchira
Kusungirako ndi moyo wa alumali
Ikhoza kusungidwa kwa nthawi yaitali pa -20 ° C;kuti mugwiritse ntchito pafupipafupi, imatha kusungidwa pa 4 ° C kwakanthawi kochepa.
