Takhala wopanga odziwa.Kupeza zambiri zama certification anu ofunikira pamsika wake wa Super Purchasing for China Real-Time PCR, Ngati mungakonde pafupifupi katundu aliyense, kumbukirani kukhala omasuka kulumikizana nafe kuti mudziwe zambiri kapena onetsetsani kuti mwatitumizira imelo molondola, tidzakuyankhani m'maola 24 okha komanso mawu abwino kwambiri operekedwa.
Takhala wopanga odziwa.Kupeza zambiri zama certification anu ofunikira pamsika wakeChina PCR, Real Time Pcr, Ndi mzimu wochititsa chidwi wa" kuchita bwino kwambiri, kuchita bwino, kuchita zinthu zatsopano ndi zatsopano", komanso mogwirizana ndi malangizo otere a "zabwino koma zamtengo wapatali," ndi "ngongole yapadziko lonse", tikuyesetsa kugwirizana ndi makampani opanga magalimoto padziko lonse lapansi kuti apange mgwirizano wopambana.

Kufotokozera

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer yomwe imatha kumasula mwachangu RNA kuchokera ku ma cell otukuka pamachitidwe a RT-qPCR, potero amachotsa njira yoyeretsera RNA yomwe imatenga nthawi komanso yovuta.The RNA template ikhoza kupezeka mu mphindi 7 zokha.5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR reagents zoperekedwa ndi zida zitha kupeza mwachangu komanso moyenera zotsatira za PCR zenizeni zenizeni.

5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR ali ndi kulekerera kwamphamvu kwa inhibitor, ndipo ma lysate a zitsanzo angagwiritsidwe ntchito ngati template ya RT-qPCR mwachindunji.Chidachi chili ndi RNA yapamwamba kwambiri ya Foregene reverse transcriptase, ndi Hot D-Taq DNA polymerase, dNTPs, MgCl.₂, zotchingira zochita, PCR optimizer ndi stabilizer.

Zofotokozera

200×20μl Rxns, 1000×20μl Rxns

Zida zamagulu

Mbali & ubwino

■ Zosavuta komanso zothandiza : ndiukadaulo wa Cell Direct RT, zitsanzo za RNA zitha kupezeka m'mphindi 7 zokha.

■ Zofuna zachitsanzo ndizochepa, mpaka ma cell 10 akhoza kuyesedwa.

■ Kutulutsa kwakukulu: kumatha kuzindikira mwachangu RNA m'maselo opangidwa mu 384, 96, 24, 12, 6-chitsime mbale.

■ DNA chofufutira akhoza kuchotsa mwamsanga matupi athu omasulidwa, kwambiri kuchepetsa zotsatira zoyeserera.

■ Makina okhathamiritsa a RT ndi qPCR amapangitsa kuti masitepe awiri a RT-PCR alembedwe bwino kwambiri komanso PCR yachindunji, komanso yosagwirizana ndi RT-qPCR reaction inhibitors.

Kit application

Kuchuluka kwa ntchito: maselo otukuka.

- RNA yotulutsidwa ndi zitsanzo za lysis: imagwira ntchito pa template ya RT-qPCR ya zida izi.

- Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kusanthula kwa jini, kutsimikizira kwa siRNA-mediated gene silence effect, kuyang'anira mankhwala, etc.

Chithunzi

Kusungirako ndi moyo wa alumali

Gawo I la zida izi ziyenera kusungidwa pa 4 ℃;Gawo II liyenera kusungidwa pa -20 ℃.

Foregene Protease Plus II iyenera kusungidwa pa 4 ℃, osazizira pa -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR iyenera kusungidwa pa -20 ℃ mumdima;ngati amagwiritsidwa ntchito kawirikawiri, amathanso kusungidwa pa 4 ℃ kuti asungidwe kwakanthawi kochepa (ntchito mkati mwa masiku 10) .Kupeza ziphaso zanu zambiri zofunika pamsika wake wa Super Purchasing for China Q160/Q320 Real-Time PCR, Ngati mungakonde pafupifupi katundu aliyense, kumbukirani kukhala omasuka kulumikizana nafe kuti mudziwe zambiri kapena onetsetsani kuti mwatitumizira imelo molondola, tidzakuyankhani mu maola 24 okha komanso ma quote abwino kwambiri adzaperekedwa.
Kugula Kwambiri kwaChina PCR, Real Time PCR, Ndi mzimu wochititsa chidwi wa" kuchita bwino kwambiri, kuchita bwino, kuchita zinthu zatsopano komanso zatsopano", komanso mogwirizana ndi malangizo otere a "zabwino koma zamtengo wapatali," ndi "ngongole yapadziko lonse lapansi", tikuyesetsa kugwirizana ndi makampani opanga magalimoto padziko lonse lapansi kuti apange mgwirizano wopambana.