Tili ndi zida zamakono.Zogulitsa zathu zimatumizidwa ku USA, UK ndi zina zotero, kusangalala ndi mbiri yabwino pakati pa makasitomala pamtengo Wabwino wa China Rapid and High Yeild Virus Total Rna Isolation Extraction Purification Kit Nucleic Acid Detection Kit, Tipitiliza kuyesetsa kukulitsa kampani yathu ndikupereka zinthu zabwino kwambiri zokhala ndi mitengo yankhanza.Kufunsa kulikonse kapena ndemanga zimayamikiridwa kwambiri.Kumbukirani kutigwira mwaufulu.
Tili ndi zida zamakono.Zogulitsa zathu zimatumizidwa ku USA, UK ndi zina zotero, kusangalala ndi mbiri yabwino pakati pa makasitomala.Automatic Nucleic Acid Kuyeretsa, China Universal Rna DNA Reagents, Timalabadira kwambiri ntchito yamakasitomala, ndipo timayamikira kasitomala aliyense.Tsopano takhala ndi mbiri yolimba m'makampani kwa zaka zambiri.Takhala oona mtima ndipo tikuyesetsa kumanga ubale wautali ndi makasitomala athu.

Kufotokozera

50 Preps, 200 Preps

Chidachi chimagwiritsa ntchito spin column ndi formula yopangidwa ndi Foregene, yomwe imatha kutulutsa RNA yapamwamba komanso yapamwamba kwambiri kuchokera ku ma cell opangidwa mu mbale 96, 24, 12, ndi 6-zitsime.

Chidachi chimapereka DNA-Cleaning Column yogwira mtima, yomwe imatha kulekanitsa ma supernatant ndi cell lysate, kumanga ndi kuchotsa DNA ya genomic.Ntchitoyi ndi yosavuta komanso yopulumutsa nthawi.

Mzere wa RNA-okha ukhoza kumangirira RNA moyenera ndi fomula yapadera.Zitsanzo zambiri zimatha kukonzedwa nthawi imodzi.

Zida zamagulu

*Chonde valani magolovesi ndikuchitapo kanthu zodzitchinjiriza mukamagwira ntchito popeza Buffer cRL1 ndi Buffer RW1 zili ndi mchere wachaotropic wokwiyitsa.

Mbali & ubwino

■ Njira yonseyi imayendetsedwa ndi kutentha kwa chipinda (15-25 ℃), popanda kusamba kwa ayezi ndi kutentha kochepa kwa centrifugation.
■ Zida zonse ndi RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
■ DNA-Cleaning Column imamangiriza DNA, kotero kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
■ Zokolola zambiri za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
■ Kuthamanga kwachangu: kosavuta kugwiritsa ntchito ndipo kumatha kumalizidwa mu mphindi 11.
■ Chitetezo: Palibe organic reagent yomwe imafunikira.
■ Ubwino wapamwamba: RNA yoyeretsedwa ndi yoyera kwambiri, yopanda mapuloteni ndi zonyansa zina, ndipo imatha kukumana ndi mayesero osiyanasiyana.

Kit application

Ndiwoyenera kuchotsa ndi kuyeretsa RNA yonse kuchokera ku maselo otukuka mu mbale 96, 24, 12, ndi 6-chitsime.

Kuyenda kwa ntchito

Chithunzi

The agarose gel osakaniza batire chithunzi cha Cell Total RNA Isolation Kit ankachitira pamwamba manambala osiyanasiyana a maselo, 20μl voliyumu elution, kutenga 2μl oyeretsedwa okwana RNA 1%.

Kusungirako ndi moyo wa alumali

Zida zitha kusungidwa kwa miyezi 12 kutentha kwa chipinda (15-25 ℃) kapena 2-8 ℃ kwa nthawi yayitali (miyezi 24).

Buffer cRL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi wa 1 mutatha kuwonjezera 2-hydroxy-1-ethanethiol (posankha) .Tili ndi zida zamakono.Zogulitsa zathu zimatumizidwa ku USA, UK ndi zina zotero, kusangalala ndi mbiri yabwino pakati pa makasitomala pamtengo Wabwino wa China Rapid and High Yeild Virus Total Rna Isolation Extraction Purification Kit Nucleic Acid Detection Kit, Tipitiliza kuyesetsa kukulitsa kampani yathu ndikupereka zinthu zabwino kwambiri zokhala ndi mitengo yankhanza.Kufunsa kulikonse kapena ndemanga zimayamikiridwa kwambiri.Kumbukirani kutigwira mwaufulu.
Mtengo wokwanira waChina Universal Rna DNA Reagents, Automatic Nucleic Acid Kuyeretsa, Timalabadira kwambiri ntchito yamakasitomala, ndipo timayamikira kasitomala aliyense.Tsopano takhala ndi mbiri yolimba m'makampani kwa zaka zambiri.Takhala oona mtima ndipo tikuyesetsa kumanga ubale wautali ndi makasitomala athu.