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Zanyama Zonse RNA Kudzipatula Kit
Chikwamacho chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwambiri komanso kwapamwamba kwambiri kwa RNA kuchokera kumatundu osiyanasiyana azinyama. Imakhala ndi gawo loyeretsetsa DNA lomwe limatha kuchotsa DNA yamagetsi kuchokera ku supernatant ndi minofu lysate. Mzere wa RNA wokha umatha kumangiriza RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Makina onsewa alibe RNase, chifukwa chake RNA yoyeretsedwa sidzakhala yowonongeka. Buffer RW1 ndi Buffer RW2 zitha kuonetsetsa kuti RNA yomwe idapezedwa siyodetsedwa ndi mapuloteni, DNA, ayoni, ndi zinthu zina.
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Cell Yonse ya RNA Yodzipatula
Chikwama ichi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwapamwamba komanso kwapamwamba kwambiri kwa RNA yonse kuchokera kumaselo opangidwa mu 96, 24, 12, ndi 6-mbale zabwino. Chikwamacho chimapereka gawo loyeserera la DNA-loyeretsa, lomwe limatha kusiyanitsa supernatant ndi cell lysate, kumangirira ndikuchotsa genomic DNA. Ntchitoyi ndi yosavuta komanso yopulumutsa nthawi; Column yokhayo ya RNA imatha kumangiriza RNA mwanjira yapadera. Ziwerengero zambiri zitha kukonzedwa nthawi imodzi.
Dongosolo lonse ndilopanda RNase, kotero kuti RNA yoyeretsedwa isanyozedwe; Buffer RW1, Buffer RW2 chotsuka chomenyera chotetezera chimapatsa RNA yopanda mapuloteni, DNA, ion, ndi kuipitsa kwamagulu.
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Viral DNA RNA Yodzipatula
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwapamwamba komanso kwapamwamba kwambiri kwa ma virus a DNA ndi RNA kuchokera kuzitsanzo monga plasma, seramu, madzi opanda thupi opanda cell, komanso supernatant yachikhalidwe. Chikwamacho chimawonjezera Linear Acrylamide, yomwe imatha kutenga ma DNA ndi RNA mosavuta kuchokera pazitsanzozo. DNA / RNA-Column yokha ndi yomwe ingamange bwino DNA ndi RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Chikwama chonsecho mulibe RNase, chifukwa chake RNA yoyeretsedwa siyingawonongeke. Buffer RW1 ndi Buffer RW2 zitha kuwonetsetsa kuti ma virus a acid omwe alibe ma protein, nuclease kapena zonyansa zina, zomwe zitha kugwiritsidwa ntchito molunjika poyesa kwamayeso a biology.
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Bzalani Chiwerengero cha RNA Isolate Plus Kit
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwambiri komanso kwapamwamba kwambiri kwa RNA kuchokera kumatumba osiyanasiyana okhala ndi polysaccharides kapena polyphenols. Imakhala ndi gawo loyeretsetsa DNA lomwe limatha kuchotsa DNA yamagetsi kuchokera ku supernatant ndi minofu lysate. Mzere wa RNA wokha umatha kumangiriza RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Chikwama chonsecho mulibe RNase, chifukwa chake RNA yoyeretsedwa siyingawonongeke. Buffer PRW1 ndi Buffer PRW2 zitha kuonetsetsa kuti RNA yomwe idapezedwa siyodetsedwa ndi mapuloteni, DNA, ayoni, ndi zinthu zina.
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Viral DNA & RNA Yodzipatula
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwapamwamba komanso kwapamwamba kwambiri kwa ma virus a DNA ndi RNA kuchokera kuzitsanzo monga plasma, seramu, madzi opanda thupi opanda cell, komanso supernatant yachikhalidwe. Chikwamacho chimawonjezera Linear Acrylamide, yomwe imatha kutenga ma DNA ndi RNA mosavuta kuchokera pazitsanzozo. DNA / RNA-Column yokha ndi yomwe ingamange bwino DNA ndi RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Chikwama chonsecho mulibe RNase, chifukwa chake RNA yoyeretsedwa siyingawonongeke. Buffer RW1 ndi Buffer RW2 zitha kuwonetsetsa kuti ma virus a acid omwe alibe ma protein, nuclease kapena zonyansa zina, zomwe zitha kugwiritsidwa ntchito molunjika poyesa kwamayeso a biology.
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Viral RNA Yodzipatula
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwapamwamba komanso kwapamwamba kwambiri kwa ma virus a RNA kuchokera kuzitsanzo monga plasma, seramu, madzi opanda thupi opanda cell, komanso supernatant yachikhalidwe. Chikwamacho chimawonjezera Linear Acrylamide, yomwe imatha kutenga RNA yaying'ono kuchokera pazitsanzozo. RNA-Column yokha ndi yomwe ingamange RNA bwino. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Chikwama chonsecho mulibe RNase, chifukwa chake RNA yoyeretsedwa siyingawonongeke. Buffer viRW1 ndi Buffer viRW2 zitha kuwonetsetsa kuti ma virus a nucleic acid omwe alibe protein, nuclease kapena zonyansa zina, zomwe zitha kugwiritsidwa ntchito molunjika poyesa kwa biology yama cell.
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Bzalani Chiwerengero cha RNA Chokha
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwambiri komanso kwapamwamba kwambiri kwa RNA kuchokera kumatumba osiyanasiyana okhala ndi polysaccharides otsika ndi polyphenols. Pazitsanzo zazomera zomwe zili ndi polysaccharides kapena polyphenols, tikulimbikitsidwa kuti mugwiritse ntchito Plant Total RNA Isolate Plus Kit kuti mupeze zotsatira zabwino za RNA. Chikwamacho chimapereka gawo loyera la DNA lomwe lingachotse mosavuta genomic DNA kuchokera ku supernatant ndi minofu lysate. Mzere wa RNA wokha umatha kumangiriza RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Makina onsewa alibe RNase, chifukwa chake RNA yoyeretsedwa sidzakhala yowonongeka. Buffer PRW1 ndi Buffer PRW2 zitha kuonetsetsa kuti RNA yomwe idapezedwa siyodetsedwa ndi mapuloteni, DNA, ayoni, ndi zinthu zina.
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Bzalani Total RNA Isolate Kit Plus
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwambiri komanso kwapamwamba kwambiri kwa RNA kuchokera kumatumba osiyanasiyana okhala ndi polysaccharides kapena polyphenols. Imakhala ndi gawo loyeretsetsa DNA lomwe limatha kuchotsa DNA yamagetsi kuchokera ku supernatant ndi minofu lysate. Mzere wa RNA wokha umatha kumangiriza RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Chikwama chonsecho mulibe RNase, chifukwa chake RNA yoyeretsedwa siyingawonongeke. Buffer PRW1 ndi Buffer PRW2 zitha kuonetsetsa kuti RNA yomwe idapezedwa siyodetsedwa ndi mapuloteni, DNA, ayoni, ndi zinthu zina.
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Bzalani DNA Yodzipatula
Chida ichi chimagwiritsa ntchito gawo lokhalo la DNA lomwe limatha kulumikiza DNA, Foregene protease ndi dongosolo lapadera lotetezera, lomwe limathandizira kuyeretsa kwa mbewu ya genomic DNA. DNA yamtundu wapamwamba kwambiri imatha kupezeka mkati mwa mphindi 30, zomwe zimapewa kuwonongeka kwa genomic DNA.
Kakhungu ka silika ka gel-DNA kokha kamene kamagwiritsidwa ntchito mu gawo la spin ndi chinthu chatsopano chapadera cha Foregene, chomwe chimatha kulumikiza ndi DNA, ndikukulitsa kuchotsedwa kwa RNA, mapuloteni osayera, ayoni, polysaccharides, polyphenols ndi mankhwala ena.
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General Plasmid Mini Kit
Chogulitsachi chimagwiritsa ntchito ukadaulo waukadaulo wa DNA-yokhayo komanso njira yabwino ya SDS lysis, yomwe imatha kupeza ma plasmid DNA apamwamba kwambiri kuchokera ku mabakiteriya mkati mwa mphindi 20.
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Zinyama Zanyama DNA Kupatula Kit
Chida ichi chimagwiritsa ntchito gawo lokhalo la DNA lomwe limatha kumangiriza DNA, Foregene protease ndi dongosolo lapadera lotetezera. DNA yamtundu wapamwamba kwambiri imatha kutulutsidwa m'maselo otukuka osiyanasiyana ndi nyama zamkati mwa mphindi 30 mpaka 50.
Kakhungu ka silika ka gel-DNA kokha kamene kamagwiritsidwa ntchito mu gawo la spin ndi chinthu chatsopano chapadera cha Foregene, chomwe chimatha kulumikiza ndi DNA, ndikukulitsa kuchotsedwa kwa RNA, mapuloteni osayera, ayoni ndi zinthu zina zamagulu. 5-80μg apamwamba a genomic DNA amatha kutsukidwa kuchokera ku 10-50mg minofu.
