Plant Seed Direct PCR Kit I/II (popanda Sampling Tools) Protocol Plant Direct PCR Kits
Kufotokozera Kwa Zida:
◮Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo (Nthawi, ntchito ndi mtengo wasungidwa!)
◮Zinthu zochepa
◮Zosavuta-chitsanzocho chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
◮2 X Mix-kuchepetsa zolakwika pakukweza zitsanzo ndi nthawi yokonzekera dongosolo
◮Kukonzekera mwachangu-template kumatha kumaliza mphindi 10, machitidwe a PCR amatha kumaliza mphindi 50
◮High throughput-lysis reaction itha kumalizidwa mu mbale ya 96-PCR
Kufotokozera
Izi zimagwiritsa ntchito njira yapadera ya lysis buffer kuti lyse mbewu za mbewu.Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsedwa.Mukawonjezera zoyambira, PCR Mix ya zida izi zitha kugwiritsidwa ntchito pakukulitsa.
Zofotokozera
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Zida zamagulu
| Plant Seed Direct PCR Kit I | |
| Gawo I | Mtengo SP1 |
| Mtengo SP2 | |
| 6 × DNA Loading Buffer | |
| Gawo II | 2 × Mbewu PCR YosavutaTMSakanizani |
| Bzalani Mbewu Direct PCR Kit II | |
| Gawo I | Mtengo SP1 |
| Mtengo SP2 | |
| 6 × DNA Loading Buffer | |
| Gawo II | 2 × Mbewu PCR YosavutaTMSakanizani |
Mbali & ubwino
■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Zosavuta-chitsanzocho chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2X Mix-kuchepetsa zolakwika zotsitsa zitsanzo ndi nthawi yokonzekera dongosolo
■ Mwachangu-Kukonzekera kwa template kumatha kutha mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50
■ Kuchita zambiri-lysis reaction imatha kumalizidwa mu mbale ya 96-PCR.
Zosintha za Kit
Ntchito: Kuzindikiritsa zomera transgenic, genotyping, etc.
Zitsanzo: Bzalani njere kapena minyewa yokhala ndi polysaccharide yochepa komanso polyphenol.
Kukula kwachitsanzo: Mbewu imodzi yathunthu, 1-100mg minyewa ya mbewu
Mitundu yodziwika: PCR product ≤1kb pa
Kuyenda kwa ntchito
Electropherogram
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