China Chomera tsamba Direct PCR kit-UNG (popanda Zitsanzo Zida) Protocol Direct PCR kuchokera Plant Material Manufacturer ndi Supplier |Foregene

Chomera tsamba Direct PCR kit-UNG (popanda Sampling Tools) Protocol Direct PCR from Plant Material

Chomera tsamba Direct PCR kit-UNG (popanda Zitsanzo Zida) Protocol Direct PCR kuchokera ku Plant Material Image Featured

Kufotokozera Kwa Zida:

Mwachindunji gwiritsani ntchito tsamba kapena tsamba la lysate ngati template yakukulitsa kwa PCR, popanda kutulutsa kosiyana kwa DNA, kufulumira komanso kukhudzika kwakukulu.Dongosolo la UNG loletsa kuwononga chilengedwe lawonjezedwa kuti tipewe zolakwika.

◮Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo (Nthawi, ntchito ndi mtengo wasungidwa!)

◮Zinthu zochepa

◮Zosavuta-chitsanzocho chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya

◮2 X Mix-kuchepetsa zolakwika pakukweza zitsanzo ndi nthawi yokonzekera dongosolo

◮Kukonzekera mwachangu-template kumatha kumaliza mphindi 10, machitidwe a PCR amatha kumaliza mphindi 50

◮High throughput-lysis reaction itha kumalizidwa mu mbale ya 96-PCR

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Kutengera zida za Plant Leaf Direct PCR, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo puloteni ya UNG yomwe ingawononge template yomwe ili ndi dUTP imawonjezedwa.Mwanjira iyi, kutsimikizika ndi kulondola kwa kukulitsa kumatsimikizika, ndipo vuto la kuipitsidwa kwazinthu za PCR zomwe zitha kuchitika pakuyesa kwakukulu kwa majini zimatetezedwa.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	Mtengo P1
	Mtengo P2
	6×DNA Loading Buffer
Gawo II	2× Leaf PCR Easy^TMMix(UNG)

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Chosavuta-chitsanzo chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2 × Mix, kuchepetsa chitsanzo Mumakonda zolakwa ndi anachita dongosolo kukonzekera nthawi
■ Kukonzekera mwachangu template kumatha kumaliza mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50.
■ High throughput-lysis reaction akhoza kumalizidwa mu 96-chitsime PCR mbale

Zosintha za Kit

Kugwiritsa ntchito: chizindikiritso chosinthidwa ma genetic, genotyping, etc.
Chitsanzo: tsamba la mbewu
Mlingo: Diameter 2-3mm (njira yolunjika), m'mimba mwake 5-7mm (njira ya lysis)
Mtundu wozindikiridwa: chidutswa chandamale ≤1kb