China Mbidzi Nsomba Mwachindunji PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Wopanga ndi Wopereka |Foregene

Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(mbidzi)

Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Kugwiritsa ntchito mwachindunji kwa zebrafish lysate ngati template ya PCR amplification, palibe kutulutsa kosiyana kwa DNA, kufulumira komanso kukhudzika kwambiri.Adawonjezera UNG anti-pollution system kuti athetse zolakwika.

◮Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

◮Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

◮Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwachangu DNA ya genomic kuchokera ku zebrafish ndi minofu ina yamadzi am'madzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuchita kwa PCR, kotero ndizoyenera kuyesa kwakukulu kwa majini.Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65 ° C.Palibe njira zina monga kuchotsedwa kwa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2 × PCR EasyTM Mix (UNG) imagwiritsa ntchito dUTP m'malo mwa dTTP pamaziko a 2 × PCR EasyTM Mix, ndikuwonjezera UNG enzyme (Uracil-N-glycosylase) yomwe ingawononge template yomwe ili ndi dUTP nthawi yomweyo.PCR isanachitike, puloteni ya UNG imagwiritsidwa ntchito kusokoneza mankhwala a PCR okhala ndi uracil.Enzyme ya UNG sidzakhala ndi zotsatirapo pa template yomwe ilibe uracil, potero imatsimikizira kutsimikizika ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesa kwakukulu kwa majini.Kuyipitsidwa kwa zinthu za PCR kunachitika.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	FP yoyipa
	Foregene Protease
	6 × DNA Loading Buffer
Gawo II	2 × PCR Yosavuta^TMMix(UNG)
Malangizo

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

■ Zofuna zachitsanzo ndizochepa, monga 1mg nsomba ya caudal fin kapena mazira 10 a nsomba angayesedwe.

■ Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo opaleshoniyo ndi yosavuta.

■ Anti-pollution PCR system 2 × PCR EasyTM Mix (UNG), yomwe imatha kuthetsa bwino kuipitsidwa ndi zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Zosintha za Kit

Kugwiritsa ntchito: Nsomba za Zebra ndi nsomba zina zam'madzi.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kayendedwe kantchito

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer FP ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

v Reagent Foregene Protease ali ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

vReagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

vReagent 2 × PCR EasyTM Mix (UNG), ngati imagwiritsidwa ntchito kawirikawiri, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (kugwiritsa ntchito mkati mwa masiku 10).

Zam'mbuyo: Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent (mbidzi)

Ena: Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent (Mchira wa Mbewa) (ya Genotyping)

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