Bzalani Mbeu(Zazikulu) Direct PCR Kit II-UNG(popanda Zida Zoyeserera)
Kufotokozera
Mankhwalawa amagwiritsa ntchito njira yapadera ya lysis reaction, yomwe imatha kumasula DNA ya genomic kuchokera ku zitsanzo za mbeu zokhala ndi polysaccharide yochepa ndi polyphenol (monga: mpunga, tirigu, fodya, chimanga, soya, etc.)Pofuna kukwaniritsa zofunikira za mayeso osiyanasiyana ozindikira, zida izi zitha kugwiritsa ntchito mitundu ingapo ya zitsanzo (monga njere zonse, zodula ting'onoting'ono, kapena zitsanzo zanthaka zingapo) poyesera.Pakati pawo, zitsanzo za mbeu zomwe zimafunikirabe kumera, mutha kusankha kudula mabala ang'onoang'ono (1-5mg) kupatulapo mluza wa mbewu kuti muyese;pazoyesera zomwe zimayenera kuyesedwa mu zitsanzo zambiri, mutha kusankha kusakaniza zitsanzo zingapo ndikuziphatikiza.Pambuyo pogaya mu tinthu tating'onoting'ono ta 0.5mm, kuyesako kutha kuchitika (osachepera 0.1% ya chitsanzo chandamale amatha kuzindikirika).
Zofotokozera
50T,200T,500T,2000T
Zida zamagulu
Bzalani Mbewu Direct PCR Kit II-UNG Bzalani Mbewu (Zazikulu) Direct PCR Kit-UNG | |||||
Kukonzekera kwa Kit | Chithunzi cha TP-0314T | Mtengo wa TP-03141 | Mtengo wa TP-03142 | Mtengo wa TP-03143 | |
50 T | 200T | 500T | 2000T | ||
Gawo I | Mtengo SP1 | 30 ml pa | 120 ml | 100ml × 3 | 400ml × 3 |
Mtengo SP2 | 3ml ku | 10 ml pa | 25ml ku | 100 ml | |
6 × DNA Loading Buffer | 1.5ml pa | 1.5ml pa | 1.5ml pa | 1.5ml × 4 | |
Gawo II | 2 × Mbewu PCR YosavutaTMMix(UNG) | 500μl | 1mlx2 pa | 1.7ml × 3 | 1.7ml × 12 |
Pamanja | 1 | 1 | 1 | 1 |
Mbali ndi ubwino
- Palibe chifukwa chotengera nthawi komanso mtengo woyeretsa DNA umafunika.
- Zofuna zachitsanzo ndizochepa, ingotengani mbeu imodzi.
- Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.
- Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolera mwamphamvu kwa PCR reaction inhibitors.
- Anti-kuipitsa PCR dongosolo 2 × Mbewu PCR EasyTMMix (UNG) imatha kuthetsa kuipitsidwa komwe kumachitika chifukwa cha zinthu za PCR ndikuwonetsetsa kuti makulidwe ake ndi olondola.
Kayendedwe kantchito
Zosungirako
1.Mayendedwe
Njira yonse yoyendetsa mabokosi otsika kutentha kwa ayezi, kuonetsetsa kuti zida Gawo II zili m'malo a <4 ℃.
2.Kusungirako
Gawo I la chida ichi liyenera kusungidwa kutentha kapena kutentha kwa 2-8 ° C.
Gawo II liyenera kusungidwa pa -20 ° C.