Madzi a DNA Isolation Kit DNA Extraction and Purification Kit for Water
Kufotokozera Kwa Zida:
Yeretsani mwachangu ma DNA apamwamba a genomic kuchokera ku tizilombo tating'onoting'ono tosiyanasiyana tamadzi.
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Zofotokozera
50 Zokonzekera
-Lysozyme: enzymatic hydrolyze cell khoma la mabakiteriya abwino.
-Buffer TE: amagwiritsidwa ntchito pokonzekera 100mg/ml Lysozyme njira ndikupereka lysozyme enzymatic hydrolysis chilengedwe.
-Buffer SG1 & Buffer SG2: Perekani chitsanzo cha kugaya kwa protease.
-Forgene Protease: Zitsanzo za Enzymatic hydrolyze mu malo a protease enzymatic kuti amasule genomic DNA.
-Buffer SG3: Yambitsani Foregene Protease ndikupereka malo odzaza DNA.
-Buffer SG4: Yowonjezera kuti ipereke malo otengera DNA.
-Buffer PW: Chotsani zonyansa monga mapuloteni ndi RNA mu DNA.
-Buffer WB: Chotsani ma ayoni amchere otsalira mu DNA.
-Buffer EB: tulutsani DNA pa membrane yoyeretsa.
-Mzere wokha wa DNA: makamaka adsorb genomic DNA mu lysate.
Zida zamagulu
| Mtengo wa SG1 |
| Mtengo wa SG2 |
| Mtengo wa SG3 |
| Mtengo wa SG4 |
| Mtengo PW |
| Mtengo WB |
| Mtengo wa EB |
| Mtengo wa TE |
| Foregene Protease |
| Lysozyme |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda RNase yowonjezera panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.
-Kuthamanga Kwambiri: Ntchitoyi ndi yosavuta, ndipo ntchito yochotsa madzi a genomic DNA imatha kutha mkati mwa mphindi 40.
- Yabwino: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
- Chitetezo: palibe chifukwa chochotsa organic reagent.
-Zapamwamba: Zidutswa za DNA zomwe zatulutsidwa ndi zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira pazoyeserera zosiyanasiyana.
Kit application
Ndi oyenera kuyeretsedwa kwa genomic DNA zitsanzo zotsatirazi: aquaculture dziwe madzi, madzi dziwe, madzi apampopi, nyanja madzi, mtsinje madzi, lotus dziwe madzi ndi zitsanzo zina.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
-Chida ichi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C), ngati chiyenera kusungidwa kwa nthawi yaitali, chikhoza kusungidwa pa 2-8 ° C.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa kutentha kwapakati kwa nthawi.Ngati ndi kotheka, itenthetseni mu 37°C madzi osamba kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
-Foregene Protease yankho lili ndi ndondomeko yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);ikasungidwa pa 4 ℃, ntchito yake ndi kukhazikika kwake kudzakhala bwino, choncho tikulimbikitsidwa kuti tisunge pa 4 ℃, kumbukirani kuti musasunge pa -20 ℃.
-Dry ufa Lysozyme amasungidwa pa -20 ° C;njira yothetsera Lysozyme imagawidwa m'magawo ang'onoang'ono ndikusungidwa pa -20 ° C.
